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Biomedical subjects

I Andersen

Publications and source records attributed to I Andersen.

At least 55 records · Page 3Linked to original sources
PubMed1982

Specificities of anti-rabbit VH antibodies in mammalian and fowl antisera.

Antibodies against rabbit VH-determinants were produced by immunizing mammals and birds with rabbit IgG, IgA or VH. Most of the anti-VH produced in mammals was specific for allotype VH-determinants, but small amounts of antibody against non-allotype VH-determinants were also produced. By contrast the immunization of chickens led to the production of predominantly non-allotype VH antibody. Further analysis of the antibody specificity revealed the production of antibody against VH-determinants available in the intact immunoglobulin (H- + L-chain) as well as antibody reacting with determinants only accessible in the absence of L-chain. Antibody of the latter specificity prevailed after immunization with VH. The purified VH was not deficient in antigen determinants when compared with VH in the intact immunoglobulin (IgA). Unexpectedly, none of our chicken anti-rabbit VH antisera showed crossreactivity with VH from other mammalian species.

Animals↗
PubMed1982

Cytomegalovirus antibodies in epileptics receiving diphenylhydantoin.

Cytomegalovirus (CMV) antibodies were determined by indirect haemagglutination in 53 epileptics receiving long-term diphenylhydantoin (DPH) therapy and in 53 matched controls. Absorption of serum IgG, IgA and IgM performed in 12 sera showed that the antibodies were of the IgG class. A decreased incidence of high CMV antibody titres (greater than or equal to 320) was found in epileptics (11.3%) compared with controls (34.0%) (0.02 greater than P greater than 0.01), whereas antibody titres greater than or equal to 40 were found in 37.7% of epileptics and in 45.3% of controls (n.s.). The CMV antibody incidence and titre range were similar in patients with symptomatic (37.9%) and idiopathic (37.5%) epilepsy, suggesting that the DPH treatment was responsible for the decreased antibody occurrence in patients. No correlation between CMV antibody titres and the serum immunoglobulin levels or the DPH concentration or clearance could be established in the epileptics.

Adolescent↗
PubMed1981

Eggs: conveniently packaged antibodies. Methods for purification of yolk IgG.

Eggs from immunized chickens may provide a convenient and inexpensive source of antibodies. We describe two simple and efficient methods for purification of IgG from yolk. The antibody is rendered useful for most currently employed immunological techniques. Amounts of antibody corresponding to almost half a litre of antiserum may be recovered from a chicken in one month.

Animals↗
PubMed1981

Gamma-glutamyltransferase, aspartate aminotransferase and alkaline phosphatase as markers of alcohol consumption in out-patient alcoholics.

Serum activity of gamma-glutamyltransferase, aspartate aminotransferase and alkaline phosphatase were determined in 316 patients attending an out-patients clinic for treatment of alcoholism. The activity of gamma-glutamyltransferase was raised in 34% and that of aspartate aminotransferase and alkaline phosphatase in 18% and 7%. Neither the activity of gamma-glutamyltransferase, aspartate aminotransferase nor alkaline phosphatase showed any significant (P greater than 0.05) correlation with the history of alcohol consumption. The activities of gamma-glutamyltransferase and aspartate aminotransferase were raised significantly more often in patients with recent alcohol consumption than in patients who had abstained for more than 9 days. The concentration of alkaline phosphatase was not significantly (P greater than 0.05) different in these groups. The predictive value of raised and normal activities of gamma-glutamyltransferase, in deciding whether a patient had had recent alcohol consumption or not, was not superior to the predictive value of raised and normal activities of aspartate aminotransferase.

Adult↗
PubMed1979

Quantitation of proteins by electroimmunoassay using a digitizer connected with a programmable calculator.

A system is described which considerably facilitates the reading and the subsequent conversion of measured values to protein concentrations, when proteins are quantitated by the electroimmunoassay a.m. Laurell (1972). The rocket heights of calibration samples and unknown samples are read by a cursor on a magnetic table (Digitizer, Hewlett Packard) and the values are automatically transferred to a programmable calculator (HP 9830 A, Hewlett Packard). It is programmed to calculate the protein concentration of samples by interpolation on a calibration curve fitted to the best polynomium of second degree by the method of least squares. The results and sequence numbers are automatically printed out from a printer (HP 9866 A, Hewlett, Packard), Reading and calculation of the results from one plate with 5 calibration samples (in duplicate) and 20 unknown samples are completed in less than 2 min. This is 10--15 times faster compared with a manual procedure where a hand-drawn calibration curve is used for interpolation.

Computers↗
PubMed1979

A study of the linkage relations of epidermolysis bullosa dystrophica.

Two large families from the Faroe Islands presenting epidermolysis bullosa of the dystrophic type were subjected to extensive linkage analyses with 22 serological markers. No significant evidence in support of linkage with any of these loci was provided. It was found to be very unlikely that the gene or genes causing the present types of epidermolysis bullosa belong to the EBS1 locus known to be closely linked to the GPT locus.

Alanine Transaminase↗
PubMed1979

Human response to controlled levels of inert dust.

We studied nasal mucous flow, airway resistance, and subjective response in 16 young healthy subjects during 5-hour exposures to 2, 10, and 25 mg of inert dust per m3 in an environmental chamber. The dust was a fully polymerized plastic dust containing carbon black. The number of these particles in room air, expressed as a per cent of the total number of particles was 36, 41, 14, 7, and 2, respectively, for the aerodynamic size ranges less than or equal to 1.8, 1.9 to 5.3, 5.4 to 8.9, 9.0 to 12.4, and greater than or equal to 12.5 micron. No significant changes in nasal mucociliary clearance rate or nasal resistance were observed. At all dust concentrations there was a decrease in 1-sec forced expiratory volume, but not in the forced vital capacity or the forced expiratory flow during the middle half of the forced vital capacity. The nasal penetration fraction of particles was approximately 55 per cent for the smallest particles and 20 per cent for the largest particles. Discomfort was proportional to the concentration of dust, but lagged almost 2 hours behind the changes in dust concentration. The discomfort was never excessive; the main complaints were dryness in the nose and pharynx.

Adult↗