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Biomedical subjects

I Iwase

Publications and source records attributed to I Iwase.

8 recordsLinked to original sources
PubMed1984

A roentgenocephalometric study of skeletal changes during and after chin cup treatment.

Skeletal changes before and during chin cup treatment and the stability of results after treatment were studied. Materials consisted of longitudinal lateral cephalometric roentgenograms of twenty-six Japanese females with skeletal Class III malocclusion treated with the chin cup. Treatment was started when patients were 6 to 9 years of age. Annual changes in linear and angular measurements before, during, and after treatment were analyzed statistically. The results were as follows: (1) There was some reduction in the amount of expected growth increment in the mandible; a change in mandibular form and distal displacement of the mandible took place during treatment. (2) There was no obvious change in the cranium and maxilla during treatment. (3) Neither acceleration nor restraint of expected growth increment was found after treatment. The exception was forward displacement of the mandible during the first year after removal of the chin cup. To illustrate these results, the skeletal changes of one individual Japanese Class III female during and after chin cup treatment were analyzed with the use of fifteen parameters indicating area ratios, linear ratios, and angles. The control sample consisting of 426 nontreated Japanese females with skeletal Class III malocclusion was observed, varying between 3 years and 30 years of age. The growth in this case showed a trend similar to that of the control sample for the observation period, but it was redirected from the expected natural growth for the treatment period.

Adolescent↗
PubMed1984

Purification by sucrose density gradient zonal centrifugation and affinity column chromatography of antigenic substances from the livers of mice infected with Tyzzer's disease.

Antigenic substances from livers of mice infected with Tyzzer's disease were purified by means of sucrose density gradient zonal centrifugation and affinity column chromatography using antiserum and checking antigenicity with the complement fixation test. Fractions obtained from zonal centrifugation fell into three main groups with different molecular weights, two of which (Fr. I and Fr. II) positively reacted with antiserum in the complement fixation tests. Both fractions were further purified by affinity column chromatography. The molecular weights of the main antigenic substances derived from Fr. I and Fr. II were determined to be about 52 000 and 66 000, respectively, by means of SDS-PAGE.

Animals↗
PubMed1983

Single radial complement fixation test using complement film. Assay of the antibody response to strain and type specific antigens of influenza virus.

A stabilized modification of the single radial complement fixation test in gel (SRCF) was developed for detecting influenza antibodies. The principle of the test is the use of a single-step procedure with the following reagents: (1) Agarose plate containing influenza antigen and antibody coated erythrocytes (EA). (2) Thin plastic film coated with dried complement. By filling the wells cut in the agar with the heat inactivated serum samples and covering the agar surface with the complement film, a zone of unlysed cells surrounded by a haemolytic area appears after overnight incubation for 16-18 h at 4 degrees C and 1-2 h at 37 degrees C. The squares of the zone diameter were measured for estimating the antibody quantity by using CF(S) and virion antigen of influenza virus, and the type-specific antibody was demonstrated by using CF(S) antigen, while the strain-specific antibody was demonstrated by using virion antigen. An excellent correlation was demonstrated for antibody titres between conventional CF and SRCF with CF(S) antigen, on the one hand, and, between conventional HI and SRCF with virion antigen, on the other.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1981

Study on the single radial complement fixation (SRC-fix) test.

We examined whether the single radial complement fixation (SRC-fix) test is applicable to serological diagnosis of viruses. The viruses used for the examination were influenza A and herpes simplex viruses. SRC-fix test was shown to be a very simple method, that is, serum samples were added to agarose plate A containing complement and CF antigen, and agarose plate B, containing antibody-coated erythrocytes, was layered on top of the plate A to form zone areas of unlysed cells. CF titres of the samples were determined from the square of the zone diameter of unlysed cells. Thus SRC-fix test is suggested as a new method for diagnosis of viral diseases.

Antibodies, Viral↗