PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 6262349

Study on the single radial complement fixation (SRC-fix) test.

S Niwayama·S Sato·H Ochiai·S Mutoda·I Iwase·K Hayashi
View original source ↗Export metadata ↓

Abstract

We examined whether the single radial complement fixation (SRC-fix) test is applicable to serological diagnosis of viruses. The viruses used for the examination were influenza A and herpes simplex viruses. SRC-fix test was shown to be a very simple method, that is, serum samples were added to agarose plate A containing complement and CF antigen, and agarose plate B, containing antibody-coated erythrocytes, was layered on top of the plate A to form zone areas of unlysed cells. CF titres of the samples were determined from the square of the zone diameter of unlysed cells. Thus SRC-fix test is suggested as a new method for diagnosis of viral diseases.

Explore related subjects

Antibodies, ViralComplement Fixation TestsHerpes SimplexHumansInfluenza A virusInfluenza, HumanSimplexvirusJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

S Niwayama, S Sato, H Ochiai, S Mutoda, I Iwase, K Hayashi. 1981. Study on the single radial complement fixation (SRC-fix) test.. https://doi.org/10.1016/0166-0934(81)90024-0

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1992

How can peptide vaccines work?

Peptide antigens frequently induce antibodies which recognise the denatured form of a protein from which their sequences are derived. However, the ability to induce antibodies which crossreact with the native, fully folded form of the protein is less commonly observed. Although there is a growing number of examples in which this is the case, the ability to predict peptides having this property is extremely limited. Given the large surface areas involved in antibody/antigen interaction it is surprising that peptides could ever induce antibodies which would recognise the native protein well enough to have biological activity, such as the neutralization of infectivity. A mechanism is proposed to explain such observations which is compatible with many of the properties of antipeptide antibodies.

Antibodies, Viral↗
PubMed1992

The conformational specificity of viral epitopes.

Four types of antigenic sites found in viruses are discussed: cryptotopes, neotopes, metatopes and neutralization epitopes. The role played by conformation on the specificity of viral epitopes is illustrated in the case of tobacco mosaic virus and influenza virus. It appears that mechanisms reminiscent of induced fit contribute to the recognition of viral epitopes by antibodies.

Antibodies, Viral↗