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Biomedical subjects

I L Chertkov

Publications and source records attributed to I L Chertkov.

At least 55 records · Page 3Linked to original sources
PubMed1989

[Effect of endothelial and smooth muscle cells and the extracellular matrix produced by them on hematopoiesis].

The influence of endothelial and smooth muscle cells and their extracellular matrices on hemopoiesis in long-term bone marrow cultures was investigated. In contract to smooth muscle cells, the enrichment of adherent cell layer with endothelial cells led to the stimulation of hemopoietic cell and granulocyte-macrophage progenitor (CFU-GM) production. The extracellular matrices of both smooth muscle and endothelial cell origin also produced a stimulating effect on the culture, although they were not so effective as endothelial monolayers. The cultures from cryopreserved bone marrow could be established only with the help of endothelial cells or any types of extracellular matrices.

Cells, Cultured↗
PubMed1989

[Long-term culture of bone marrow from patients with PH'-positive chronic myeloid leukemia].

Long-term cultures from bone marrow of patients with chronic myeloid leukemia were obtained. The cultures were characterized by the reduced period of hemopoiesis. The enrichment of the adherent cell layer with endothelial cells or their extracellular matrix was not stimulative. The Ph'(+) malignant cells persisted in culture during the whole period of hemopoiesis. The data obtained indicate that the long-term bone marrow culture system is not suitable for the elimination of malignant cells from leukemic bone marrow.

Bone Marrow↗
PubMed1988

[The proliferative potential of hematopoietic stem cells (CFUs) during serial passage in irradiated mice as foci of ectopic hematopoiesis].

During serial passages of hemopoietic tissue as a whole ectopic hemopoietic site under renal capsule of irradiated mice gradual decrease of proliferative potential of CFUs has been observed. The results suggest that the most probable cause of such decline is random loss of precursor cells with high but limited proliferative potential during serial passages of hemopoietic tissue.

Animals↗
PubMed1985

[Renewal of hematopoietic stem cell clones in long-term bone marrow cultures].

In long-term cultures of murine bone marrow, clonal succession of hemopoietic cells was observed as measured by karyologic analysis. There were high oscillations in self-renewal of CFUs in the cultures. A close correlation between the CFUmix karyotype and mitotic non-adherent cells in culture (but not between these cell types and CFUs) was revealed.

Animals↗
PubMed1984

[Hybrid resistance in a long-term bone marrow culture and in foci of ectopic hematopoiesis formed in the culture].

The lack of hybrid resistance to the bone marrow graft has been demonstrated in a long-term bone marrow culture. After adherent cell layer transplantation into the body the hybrid resistance was demonstrated in de novo formed ectopic hemopoietic foci. The resistance was of the recipient type, because of which the existence of the migrating component of the hemopoietic microenvironment is suggested.

Animals↗