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Biomedical subjects

I L Chertkov

Publications and source records attributed to I L Chertkov.

At least 73 records · Page 4Linked to original sources
PubMed1984

[Progenitors of transitory spleen colonies in mouse embryonal liver].

The transitory nature of about half of spleen colonies macroscopically detectable in the spleen 7 days after injection of embryonal liver hemopoietic cells was demonstrated by localization of the colonies on the spleen surface and by the study of the content of polypotential and unipotential precursors in individual 7- and 11-day colonies produced in the spleen of irradiated mice by the cells of early (12-13-day) and late (17-18-day) embryonal liver.

Animals↗
PubMed1983

[Possibility of using hematopoietic stem cells in relation to their generation age].

A hypothesis of the use of hemopoietic stem cells with a view of satisfying the demand for mature cells depending on their generation age predicts that CFUs that survived after repeated treatment with hydroxyurea should have a greater capacity for self-renewal. It has been demonstrated that after repeated administrations of hydroxyurea according to the scheme devised by the authors of the hypothesis (3-4 times) and as a result of a more prolonged treatment (6 times, every other 12 or 15 h), the capacity of the survived hemopoietic stem cells for self maintenance was not only lower than normal but commonly significantly decreased. The generation-age hypothesis of the use of hemopoietic stem cells thus remains badly needing experimental support.

Animals↗
PubMed1983

[Possibility of an artefact in determining hematopoietic stem cell proliferation by CFUs suicide methods].

Spleen colonies in the irradiated mice are produced by both stem cells and by their more differentiated progeny. In the latter case the colonies are transitory, ceasing 10-11 days after cell injection. The transitory colonies may be the cause of systematic artifact during the determination of stem cell proliferation. It was shown in particular that the proliferation of stem cells after sublethal irradiation remains the same, while higher rates of suicide are determined by the death of the precursors of the transitory colonies. At the same time higher proliferation of stem cells is not artifact in lethally irradiated animals and is also detectable after exclusion of the effects of the precursors of the transient colonies.

Animals↗
PubMed1982

[Importance of cell contacts for the differentiation of the precursor cells of hematopoietic stroma in long-term bone marrow cultures].

The composite adherent cell layer is produced in the culture of bone marrow fragments. On the contrary, in the culture of single cell suspension of bone marrow, only of monolayer of fibroblast-like cells and macrophages is created. After implantation of the adherent cell layer from the Dexter type culture but not from "fibroblast" culture beneath the renal capsule of syngeneic recipients the ectopic hemopoietic sites are formed. Regeneration of the adherent cell layer may occur only in the course of multilayer formation, i.e. one week after bone marrow transplantation but not three weeks after the formation of the adherent cell layer.

Animals↗