Biomedical subjects

I Pinilla

Publications and source records attributed to I Pinilla.

42 records · Page 3Linked to original sources

PubMedDate not supplied

Genetic markers in primary open-angle glaucoma.

PURPOSE: To investigate possible associations between genetic markers and Primary Open-Angle Glaucoma (POAG). METHODS: A number of genetic markers were typed in 84 unrelated patients with POAG and compared with a random sample of healthy individuals. The markers were Transferrin, Group Specific Component, G1m (1), G1m (2) and G3m (5) Allotypes, Adenylate Kinase, Adenosin Deaminase, Glyoxalase I and Acid Phosphatase and PCR-based markers HLA-DQA1 and D1S80. RESULTS: No significant differences were found except the strong association between the group of POAG patients and Acid Phosphatase ACP*C allele (chi 2 = 32.86; p < 0.0001). CONCLUSIONS: Since Acid Phosphatase gene is localized to chromosome 2p23, this result could be a first comprehensive step in the localization of POAG genes.

Acid Phosphatase↗

PubMedDate not supplied

Histological toxicity of grepafloxacin after intravitreal injection.

PURPOSE: To determine the intravitreal levels of grepafloxacin after intravitreal injection of 80 microg and to evaluate the retinal toxicity after intravitreal injection of different doses of grepafloxacin in rabbit eyes. METHODS: Fifteen female New Zealand white rabbits and 15 female pigmented 'Gigantes de España' rabbits were injected with 80 microg of grepafloxacin into the vitreous cavity. The grepafloxacin concentration was determined with HPLC after 2, 4, 8, 12 and 24 h. Eighteen female rabbits (9 New Zealand white rabbits and 9 pigmented 'Gigantes de España' rabbits) were used for a study of toxicity. The rabbits were divided into 6 treatment groups: group 1 (3 pigmented rabbits) received an intravitreal injection of 80 microg of grepafloxacin in 0.1 ml of saline solution, group 2 (3 white rabbits) 80 microg of grepafloxacin in 0.1 ml, group 3 (3 pigmented rabbits) 800 microg of grepafloxacin, group 4 (3 white rabbits) 800 microg of grepafloxacin, group 5 (3 pigmented rabbits) and group 6 (3 white rabbits) 0.1 ml of saline solution. Clinical examination was performed prior to injection and 24 h and 10 days after surgery. The animals were sacrificed 10 days after the injection, and the eyes were enucleated and fixed for histopathology. The specimens were stained with hematoxylin-eosin and toluidine blue. RESULTS: No relevant complications were found during the clinical follow-up. All the eyes showed no abnormalities in the histologic evaluation. CONCLUSION: Grepafloxacin can be considered as a safe alternative for intravitreal injection for the treatment of intraocular infections.

Animals↗

PubMedDate not supplied

Short-wavelength automated perimetry and neuroretinal rim area.

PURPOSE: To determine the correlation between neuroretinal rim area and functional losses detected by short-wavelength automated perimetry (SWAP) in a population of patients with suspected glaucoma. METHODS: Forty-two eyes of 42 ocular hypertensive subjects who met the selection criteria (intraocular pressure greater than 21 mm of Hg and normal conventional visual fields) were studied. A planimetric optic nerve head study was performed, determining the total and sectorized neuroretinal rim areas. SWAP was also done, with a modified Humphrey field analyzer. RESULTS: There were no significant correlations between the neuroretinal rim areas and the global perimetric parameters. However, the correlations between the inferotemporal neuroretinal rim area and some superonasal visual field regions (areas 3 and 4) were significant. CONCLUSIONS: There is a relation in the topography of some visual field areas assessed by SWAP and the inferotemporal neuroretinal rim area, which may play a role in the diagnosis and follow-up of suspected glaucoma.

Adult↗

PubMedDate not supplied

Glaucomatous damage patterns by short-wavelength automated perimetry (SWAP) in glaucoma suspects.

PURPOSE: To determine the glaucomatous visual field damage patterns by short-wavelength automated perimetry (SWAP) in glaucoma suspects, and to compare the frequency of diffuse visual field losses and localized defects. METHODS: 157 eyes of 157 ocular hypertensive subjects who met the selection criteria (intraocular pressure greater than 21 mm Hg and normal standard visual fields) were studied. SWAP was done with a modified Humphrey Field Analyzer. Total (TD) and Pattern Deviation (PD) probability maps were calculated for SWAP. The frequency of abnormlities in the TD and PD were determined, analyzing the visual field loss components. RESULTS: The involvement of the test points was more frequent on the TD plots than on the PD plots for all levels of defects (p< 0.001). The glaucomatous defects also showed certain topographical distribution. CONCLUSIONS: A diffuse sensitivity component of visual field loss was found at all SWAP defect depths in glaucoma suspects.

Adult↗

PubMedDate not supplied

Alpha-tocopherol derivatives and wound healing in an experimental model of filtering surgery.

OBJECTIVE: To describe the effect of alpha-tocopherol derivatives (acetate and acid-succinate) on the histopathological characteristics of the surgical fistula in an experimental model of filtering surgery. MATERIALS AND METHODS: Thirty pigmented rabbits were divided into 3 groups. Twenty-four hours before surgery the animals were injected subconjunctivally with 0.5 mL of solution that depended on the group of treatment: Group 1 (n = 10) 0.75% ethanol in balanced salt solution (BSS); Group 2 (n = 10) 100 microg alph-tocopherol-acetate in 0.75% ethanol in BSS; Group 3 (n = 10) 100 microg alpha-tocopheryl-acid-succinate in 0.75% ethanol in BSS. Histological findings were evaluated 30 days after surgery. RESULTS: The groups treated with alpha-tocopherol derivatives showed a higher percentage of persistence of the fistula and better intraocular pressure (IOP) control. CONCLUSIONS: Alpha-tocopherol derivatives showed antiproliferative properties in this experimental model of filtering surgery.

Animals↗

PubMedDate not supplied

Bilateral optic neuritis in occupational exposure to trichloroethylene.

Central nervous system depression is the predominant response to acute trichloroethylene exposure. Cranial or peripheral nerves are also affected. The paper is a case report of a bilateral retrobulbar optic neuritis caused by occupational trichloroethylene poisoning. The urinary concentration of trichloroacetic acid one week after the onset of symptoms was positive. Visual fields and visual evoked potentials recovered eight months later. Other neurological diseases, such as multiple sclerosis were excluded.

Acute Disease↗