Biomedical subjects
Publications and source records attributed to I Takanami.
The desirability is considered of an alternative to dinitrochlorobenzene (DNCB) in assessing immune competence by skin testing. The choice of an alternative allergen is discussed, taking into account the different concentration which would be necessary for weaker allergens. The alternative proposed is 1-(2,6,6-trimethyl-2-cyclohexen-1-yl)-2-butenone (TMCHB).
The authors encountered a case of primary liver cell carcinoma which metastasized to the abdominal wall at a site corresponding to the location of an indwelling catheter for intra-arterial injections. The patient was a 54-year-old male with primary liver cell carcinoma in the posterior inferior segment. Because of a subsegmentectomy of the liver on September 25, 1986 and postoperative chemotherapy, a catheter for intra-arterial injections was inserted from the right gastroepiploic artery. After anticancer agents were administered postoperatively via this catheter, the same catheter was implanted subcutaneously before discharge. From the latter part of June 1987 after discharge, there was an increase in alpha FP and a subcutaneous tumor, which gradually increased in size, was found at a site in the right hypochondrium corresponding to the location of the arterial catheter. Angiography revealed a tumor nourished by the right 10th intercostal artery, and an operation was performed to remove this tumor. From a pathological examination, this case was diagnosed as metastatic liver cell carcinoma (metastasis to the abdominal wall). The tumor was not connected with the liver and matched the site in the abdominal wall where the arterial catheter was embedded. Therefore, implantation of a catheter cannot be ruled out as a mode of metastasis other than the blood stream.
Twelve patients with pulmonary metastases of recurrent colorectal carcinoma underwent pulmonary resection at National Ohkura Hospital. The Cumulative five-year survival rate was 60 per cent. No correlation was found between the disease-free interval and survival following thoracotomy. There was a statistically significant difference in survival rates corresponding to Duke's classification of the primary lesion. Patients with solitary lesions had a better survival rate than patients with multiple lesions. We believe that the surgical treatment of pulmonary metastases in a select group of patients, i.e., those with solitary pulmonary lesions, will prolong survival and be curative in a number of patients.
Costus root oil is a new primary sensitizer for examination of the delayed cell-mediated immune system. Histologic findings of skin producing a positive reaction to costus root oil show accumulation of lymphoid cells. If the delayed hypersensitivity reaction caused by costus root oil were to be used as a means of aggregating lymphoid cells, these cells would attack cancer cells in a non-specific manner. In the present study, BALB/c mice were injected with 20% costus root oil. Fourteen days later, Meth-A tumor cells were transplanted subcutaneously, 3% costus root oil was injected around the transplanted tumor, and the effects on suppression of tumor growth were studied. The results indicated that the challenge of costus root oil had inhibited growth of the tumor in the sensitized group compared with the controls. It is anticipated that costus root oil will be quite promising as a non-specific agent for cancer immunotherapy.
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Costus root oil is a new primary sensitizer for examining the delayed, cell-mediated immune system. Ninety-nine patients with lung cancer were tested for skin reactions to costus root oil. The incidence of positive reactions to costus root oil decreased with the progress of lung cancer. The percentage of lung cancer patients who survived was significantly greater for positive than for negative patients. This study shows that skin reactions to costus root oil are related the tumor growth and prognosis in lung cancer.
Costus root oil is a new primary sensitizer to examine the delayed, cell-mediated immune system. The peripheral blood lymphocyte count has been measured in 68 cases of carcinoma of the stomach and colon. The count has been correlated with the stage of tumor spread and the patient's delayed hypersensitivity response to costus root oil. A statistically significant correlation was found between the peripheral blood lymphocyte count and the response to costus root oil. Patients with low peripheral blood lymphocyte counts tended to have more advanced tumors and a poor response to costus root oil.
Results of methyl heptine carbonate skin tests were compared with DNCB skin test results in 155 patients with chest diseases. Reactions to methyl heptine carbonate and DNCB were similar in 142 of 155 patients (92%). Forty-two lung cancer patients and 42 control patients were tested for their reactions to methyl heptine carbonate. The reactions were positive in 52% of the lung cancer patients and in 98% of the controls (p less than 0.01). The incidence of methyl heptine carbonate reactions decreased as the lung cancer stage advanced. The 6-month mortality rate was 10% in nonresponders compared to 77% in responders to methyl heptine carbonate (p less than 0.01). This study shows that reactions to methyl heptine carbonate correlate with tumor stage and prognosis in lung cancer.
Delayed hypersensitivity skin responses to costus root oil were measured in patients with colon, stomach, breast and lung cancer and in healthy controls. Cancer patients were staged according to tumor spread. Differences were demonstrated between cancer patients and controls. Patients with localized cancer showed no impairment, but diminished responses did occur in patients with advanced or metastatic cancer. There were no significant differences among any cancer groups with lymph node involvement. In the late cancer stage, costus root oil sensitivity was lost to a greater extent in patients with gastric cancer than in those with colon cancer.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Urokinase-type plasminogen activator (u-PA) is known to be involved in proteolytic degradation of extracellular matrix during carcinoma invasion. Similarly Ets-1, a transcription factor, is also known to be important for carcinoma progression, and has been reported to interact with the u-PA gene enhancer. We used immunohistochemistry to analyze the relationship between Ets-1 and u-PA protein expression in patients with pulmonary adenocarcinoma. A significant association was found between Ets-1 expression and u-PA expression. The Ets-1 expression was correlated with T factor, lymph node metastasis and stage. Kaplan-Meier survival analysis revealed that survival times were longer in patients with Ets-1-negative tumors than in patients with Ets-1-positive tumors. Our results suggest an important role for Ets-1 in tumor progression, and the correlation with u-PA protein expression serves as an important prognostic factor in pulmonary adenocarcinomas.
The expression of hepatocyte growth factor (HGF) and its receptor, c-Met, was investigated immunohistologically in tissue specimens of patients with a pulmonary adenocarcinoma. The prognosis was significantly worse in the HGF-positive or c-Met-positive patients than in the negative patients. Multivariate analysis showed that c-Met had a significant effect on the prognosis, whereas HGF did not. Our findings suggest that HGF and c-Met play an important role in tumor progression and that c-Met can be a useful prognostic marker for pulmonary adenocarcinomas.
The transforming growth factor (TGF)-beta1, TGF-beta2 and TGF-beta3 expressions were immunohistologically studied in tissues specimens from 120 patients with a pulmonary adenocarcinoma. The results of a univariate analysis indicated that the expression of each of the TGF-beta isoforms correlated with the prognosis. Of the three prognostic factors examined using Cox's stepwise regression model, the TGF-beta1 isoform was found to correlate with survival. These findings suggest that TGF-beta isoforms play an important role in tumor progression and that the TGF-beta1 isoform is a useful prognostic marker for pulmonary adenocarcinomas.
The prognostic significance of Thomsen-Frienden-reich antigen (T antigen) expression was investigated in 126 patients with pulmonary adenocarcinoma by immunohistochemical staining of tumor specimens with a monoclonal antibody specific for T antigen. T antigen immunoreactivity was detected in tumor tissue from 60 (48%) patients. T antigen expression did not correlate with gender, tumor size, presence of lymph node or distant metastasis, stage of disease, or degree of tumor cell differentiation. However, the presence of T antigen immunoreactivity correlated significantly with overall survival. The presence of T antigen immunoreactivity in tumor cells is a useful marker of prognosis in patients with pulmonary adenocarcinoma.