Formation of dendrimeric RNA assemblies though RNA loop-loop interactions.
We describe the construction of nano-scale dendritic assemblies using RNA building blocks based on dimerization initiation site (DIS) RNA of the human immunodeficiency virus type 1 (HIV-1) that forms stable base pairing through loop-loop ("kissing") interactions. RNA building blocks containing three DIS-like hairpins connected by a two nucleotide linker self-assembled in a magnesium-dependent manner to form dendrimeric structures as observed by non-denaturing polyacrylamide gel electrophoresis (PAGE).
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