Biomedical subjects

J Adline

Publications and source records attributed to J Adline.

3 recordsLinked to original sources

PubMed1988

Rapid and precise micro-methods for quantitating active components in commercial bone scintigraphy kits--II. Diphosphonates.

Two parallel, independent micro-methods for diphosphonate determination are presented. These procedures are based on the interference of diphosphonate with the formation of cation-morin (3,5,7,2',4'-pentahydroxyflavone) complexes. In the spectrophotometric method, a change of the absorbance of thorium-morin complex is used as a measure of diphosphonate concentration. In the fluorimetric method, a decrease of the fluorescence of aluminium-morin complex is used. Both methods: (1) quantitate methylene-diphosphonate in the range of 5-40 x 10(-9) M with a coefficient of variation of less than 1%; (2) are specific and interference-free in commercial kits control; (3) are operative with other diphosphonates; and (4) are adaptable to the analysis of chromatographic eluents.

Bone and Bones↗

PubMed1988

Rapid and precise micro-methods for quantitating active components in commercial bone scintigraphy kits--I. Stannous tin.

Stannous tin-diphosphonate u.v. absorbing complexes have been quantitated at appropriate wavelengths in the presence of an excess of diphosphonate ligand and chloride ions, under a nitrogen atmosphere. Results in the range of 10-80 x 10(-9) At-g of Sn(II) per sample were obtained within 2 min, with a coefficient of variation of 0.7%.

Bone and Bones↗

PubMed1979

Stereochemical aspects of the metabolism of 5-(4'-fluorophenyl)-5-phenylhydantoin in the rat.

Racemic 5-(4'-fluorophenyl)-5-phenylhydantoin was synthesized to examine its metabolism in rat. This compound differs from the antiepileptic agent 5,5-diphenylhydantoin in that the normal site of hydroxylation in 5,5-diphenylhydantoin is blocked on one of the phenyl groups by a fluorine atom. The 4'-fluoro analogue gives a major metabolite, which was isolated and identified as (R)-(-)-5-(4'-fluorophenyl)-5-(4'-hydroxyphenyl)hydantoin of 37% enantiomeric purity. The absolute configuration and enantiomeric purity of the metabolite was determined by chemical conversion to (S)-(-)-5-(4'-hydroxyphenyl)-5-phenylhydantoin. A second metabolite of the catechol type, possibly as a mixture of 5-(3',4'-dihydroxyphenyl)-5-(4'-fluorophenyl)hydantoin, and the corresponding O-3'-methyl derivative was detected by gas chromatography-mass spectrometry after methylation.

Animals↗