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PubMed · 2848784

Rapid and precise micro-methods for quantitating active components in commercial bone scintigraphy kits--II. Diphosphonates.

B Gallez·P Osinski·J Adline·P Dumont
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Abstract

Two parallel, independent micro-methods for diphosphonate determination are presented. These procedures are based on the interference of diphosphonate with the formation of cation-morin (3,5,7,2',4'-pentahydroxyflavone) complexes. In the spectrophotometric method, a change of the absorbance of thorium-morin complex is used as a measure of diphosphonate concentration. In the fluorimetric method, a decrease of the fluorescence of aluminium-morin complex is used. Both methods: (1) quantitate methylene-diphosphonate in the range of 5-40 x 10(-9) M with a coefficient of variation of less than 1%; (2) are specific and interference-free in commercial kits control; (3) are operative with other diphosphonates; and (4) are adaptable to the analysis of chromatographic eluents.

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Bone and BonesDiphosphonatesHumansMethodsRadionuclide ImagingReagent Kits, DiagnosticTechnetium Tc 99m MedronateJournal Article

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BibTeXRIS

B Gallez, P Osinski, J Adline, P Dumont. 1988. Rapid and precise micro-methods for quantitating active components in commercial bone scintigraphy kits--II. Diphosphonates.. https://doi.org/10.1016/0883-2889(88)90047-0

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