Biomedical subjects

J Błaszczyk

Publications and source records attributed to J Błaszczyk.

At least 19 recordsLinked to original sources

PubMed2001

[Evaluation of oxygen free radical generation and antioxidative enzymatic activity in blood of patients with carcinoma of the larynx (preliminary report)].

The aim of the study were the evaluation of generation of oxygen free radical and antioxidative enzymatic activity in blood of patients with carcinoma of larynx (7 persons, aged 48-68) in comparison with healthy persons (15, aged 21-28). The superoxide anion generation of blood granulocytes described by the rate of the reducted cytochrom C at rest and after stimulation with opsonized zymosan according to Bellavite et al. were measured. The enzymatic activity of superoxide dismutase in blood (method of Misra and Fridovich), catalase (method of Beers and Sizer) and malonyl dialdehyde (method of Placer et al.). The obtained data pointed at the growth of generation of oxygen free radical in blood (at rest and after stimulation with opsonized zymosan) of patients with carcinoma of larynx in comparison with the healthy ones. At the same patients with carcinoma of larynx observed at significant reduction of antioxidative enzymatic activity against the oxygen species generation in blood and significant growth of peroxidation lipids and disturbances of function of cells the human body.

Adult↗

PubMed2001

[Generation of reactive oxygen species by peripheral blood neutrophils in patients with pulmonary tuberculosis].

Oxygen metabolism of neutrophils was measured using chemiluminescence method in patient with pulmonary tuberculosis. Patients were included into three groups. Group I--20 patients (10 men, 10 women) aged 24-74 years (mean 48.3 years) with pulmonary tuberculosis BK(+). Group II--20 patients (15 men, 5 women) aged 19-67 years (mean 45.1 years) with pulmonary tuberculosis BK(0). The control group consisted 16 clinically healthy persons (12 men, 4 women) aged 28-59 years (mean 42.5 years). Blood samples (5 ml) were collected for examination from cubital vein early morning before breakfast. A luminometer 1251 coupled with an IBM PC AT compatible computer was used for the measurement of chemiluminescence. The chemiluminescence of non-stimulated neutrophils and those stimulated by a receptor stimulus formyl-Met-Leu-Phe (fMLP) as well as an extrareceptor stimulus phorbol myristate acete (PMA) was measured. The results of our study demonstrated that chemiluminescence of non-stimulated as well as stimulated by fMLP and PMA neutrophils was lower in comparison to the values of chemiluminescence in the control group.

Adult↗

PubMed2001

[Estimation of plasma malonyl dialdehyde concentration in patients with pulmonary tuberculosis].

Plasma malonyl dialdehyde (MDA) concentration was determined by Placer method in patients with pulmonary tuberculosis before and during treatment period with tuberculostatic drugs. Patients were divided into three groups. Group I comprised 20 patients (10 men and 10 women) aged 24-74 years (mean 48.3 years) with pulmonary tuberculosis BK(+). Group II comprised 20 patients (15 men and 5 women) aged 19-67 years (mean 45.1 years) with pulmonary tuberculosis BK(0). The control group consisted of 16 clinically healthy persons (12 men and 4 women) aged 28-59 years (mean 42.5 years). In patients blood samples (5 ml) were collected for examination from cubital vein before, after 1-month and 2-month treatment period with tuberculostatic drugs. In the control group blood samples were collected from cubital vein once. Results of our study showed that plasma MDA concentrations in patients with pulmonary tuberculosis, both before and during treatment period with tuberculostatic drugs, were significantly higher, in comparison to the control group.

Adult↗

PubMed2000

X-ray and 13C solid-state NMR studies of N-benzoyl-phenylalanine.

A crystalline sample of N-benzoyl-DL-phenylalanine 1 and a polycrystalline sample of N-benzoyl-L-phenylalanine 2 were studied using 13C high-resolution solid-state NMR spectroscopy. The X-ray structure of the DL form was established. Sample 1 crystallizes in a monoclinic form with a P21/c space group, a=11.338(1) A, b=9.185(1) A, c=14.096(2) A, beta=107.53(3) degrees, V=1400(3) A3, Z=4 and R=0.053. The principal elements of the 13C chemical shift tensors deltaii for 1 and 2, selectively 13C (99%) labeled at the carboxyl groups were calculated. On the basis of 13C (delta)ii analysis the hydrogen bonding pattern for sample 2 was deduced. Enriched samples were used to establish the intermolecular distance between chemically equivalent nuclei for 1 and spatial proximity in heterogeneous domain for 2, employing the ODESSA pulse sequence. The consistence of the complementary approach covering X-ray data, analysis of the 13C (delta)ii parameters and ODESSA results is revealed.

Carbon Isotopes↗

PubMed2000

[Antioxidant enzymatic activity in blood of patients with chronic hypertrophy of the maxillary sinuses and larynx and the carcinoma of the larynx].

The aim of the work was the examinations of antioxidant enzymatic activity in blood of patients with chronic hypertrophic of maxillary sinusitis (15 patients) and laryngitis (10 patients), and carcinoma of larynx (7 patients) in comparison with healthy persons (15). The enzymatic activity of superoxide dismutase in blood (method of Misra and Fridovich), katalase (method of Beers and Sizer) and malonyl dialdehyde (method of Placer) were evaluated. The obtained results pointed at significant reduction of antioxidant enzymatic activity against the oxygen species generation in blood of patients with chronic hypertrophic of maxillary sinusits and laryngitis and mainly with the carcinoma of larynx (in comparison with healthy persons). At the same patients observed the significant growth of peroxydation lipoid and disturbances of function of cells the human body.

Adult↗

PubMed2000

[Assessment of nitric oxide level in aqueous humor under physiological conditions and after lens extraction with PMMA during experimental work in rabbits].

PURPOSE: To evaluate the presence of nitric oxide and measure its level in the aqueous humor of the rabbit's eye, in physiological conditions and after extracapsular lens extraction and PMMA artificial lens implantation. We also investigated nitric oxide maintenance during early postoperative period (between 1-5 day after surgery). MATERIAL AND METHODS: We used 30 rabbits (weighing 3.0-3.5 kg) Just before surgery samples of aqueous humor were aspirated by anterior chamber puncture. Lens was extracted with extracapsular (envelope) technique. In 15 eyes PMMA IOL was implanted in the bag and 15 eyes were left aphakic. The aqueous samples were collected on 1st, 3rd, 5th days after surgery. Nitric oxide in each sample was measured with respect to fluorometric assay. RESULTS: In aqueous humor in physiological conditions we detected nitric oxide. Its level was estimated on the value of 26.52 nM/dl. After extracapsular lens extraction in both groups the level of nitric oxide was significantly higher than in control group. The day and value of NO level was different among examined groups. Nitric oxide level diminished significantly on 5th postoperative day. CONCLUSION: We came to conclusion that after ECCE and PMMA IOL implantation NO level was significantly higher as compared with control. This higher NO level after lens extraction can be responsible for the blood aqueous breakdown.

Animals↗

PubMed2000

[Assessment of nitric oxide level in aqueous humor under physiological conditions and after lens extraction and artificial foldable acrylic lens implantation during experimental work in rabbits].

PURPOSE: To evaluate the presence of nitric oxide (NO) and its level in the aqueous humor of rabbit's eye physiological conditions and after phacoemulsification and acrylic foldable artificial lens implantation. We also investigated nitric oxide maintenance during early postoperative period (between 1-5 days after surgery). MATERIAL AND METHODS: We examined 30 rabbits (weighing 3.0-3.5 kg) Just before surgery samples of aqueous humor were aspirated. Lens was extracted with phacoemulsification technique. In 15 eyes acrylic foldable IOL (group III) was implanted and 15 eyes were left aphakic (group IV). The aqueous samples were collected on 1, 3, 5 days after surgery. Nitric oxide in each sample was determined with fluorometric assay. RESULTS: The level of NO in aqueous humor in physiological conditions was estimated to 26.52 nM/dl. After phacoemulsification in both groups the level of nitric oxide was higher than in control group. The day and value of the highest NO level was different among examined groups. The highest level of NO was released during 1st day in group III and on the 3rd day in the group IV. CONCLUSION: We came to conclusion that the level of nitric oxide in aqueous humor after phacoemulsification in both groups is higher than in control group but significantly lower than in previously examined groups in which the surgery was made with extracapsular technique.

Animals↗

PubMed1998

Effect of aminoguanidine on the generation of superoxide anion and nitric oxide by peripheral blood granulocytes of rats with streptozotocin-induced diabetes.

The effect of aminoguanidine (AG) on the production of superoxide anion O2*- and nitric oxide (NO) by peripheral blood granulocytes of rats with streptozotocin-induced diabetes was studied. Induction of diabetes resulted in an increase of O2*-. Generation by both unstimulated and opsonized zymosan-stimulated granulocytes was maximal after 6 weeks and lower after 12 weeks. Treatment with aminoguanidine (1 g/l drinking water) decreased O2*- generation after 6 weeks but not after 12 weeks. NO production by both unstimulated and opsonized zymosan-stimulated granulocytes was elevated in diabetic rats to a comparable extent after 6 and 12 weeks. AG attenuated this increased NO production. These results point to the beneficial effect of AG on oxidative stress in experimental diabetes and suggest an antioxidant effect of AG.

Animals↗

PubMed1998

X-ray and high resolution selenium-77 solid state NMR spectroscopy as complementary probes to structural studies of organophosphorus diselenides.

77Se high resolution solid state NMR spectroscopy was employed to study structural properties of bis(diisopropoxyphosphorothioyl) diselenide 1 and bis(dineopentoxyphosphorothioyl) diselenide 2. The principal elements Tii of 77Se effective dipolar/chemical shift tensor were calculated from spinning sideband intensities employing the WIN-MAS program. The values of anisotropy and asymmetry parameters reflect the distortion of the selenium environment. It was found that the T33 component mostly contributes to changes in the isotropic chemical shifts. 77Se CP/MAS experiments were used to decide the assignment of space group by counting the number of crystallographically unique selenium centers in the unit cell. Crystals of diselenide 1 are triclinic, space group P1 with a = 8.485(3) A, b = 8.508(1) A, c = 8.511(2) A, alpha = 98.835(15) degrees, beta = 111.653(24) degrees, gamma = 93.524(21) degrees, V = 559.5(3) A3, Dc = 1.544(2) g/cm3 and Z = 1. Refinement using 2222 reflections for 157 variables gives R = 0.037. Crystals of diselenide 2 are triclinic, space group P1 with a = 9.1418(8) A, b = 9.1465(8) A, c = 9.9200(9) A, alpha = 74.751(8) degrees, beta = 74.629(7) degrees, gamma = 82.216(7) degrees, V = 769.7(1) A3, Dc = 1.365(2) g/cm3 and Z = 1. Refinement using 3316 reflections for 297 variables gives R = 0.0272.

Humans↗

PubMed1998

[Changes in the antioxidant system of the vitreous in rabbits after administration of sulfur hexafluoride].

PURPOSE: To evaluate the enzymatic activity of antioxidant system of rabbit's vitreous after sulfur hexafluoride (SF6) application. MATERIAL AND METHODS: Activity of CuZn-SOD, catalase and concentration of MDA in fluid and gel fraction of vitreous were determined in 24 rabbits of New Zealand race on the 2nd, 7th and 14th day after SF6 application. Control group consisted of 6 animals which did not undergo any operations. RESULTS: Dismutase and catalase activity as well as MDA concentration were higher in fluid fraction than in gel fraction in animals of control group. After SF6 application the activity of enzymes and MDA concentration did not change, whereas in fluid fraction all these values were statistically significantly reduced in all time intervals. CONCLUSIONS: SF6 leads to disintegration of vitreous structure especially just after its application. Damage to hyalocytes causes dysfunction of enzymatic system. Specific fluid fraction structure and insufficient number of substrates for peroxidation processes are the reasons for simultaneous reduction of MDA concentration.

Animals↗

PubMed1998

[Changes in the antioxidant system of the aqueous humor, lens and erythrocytes after sulfur hexafluoride application to the vitreous of rabbits].

AIM: To determine the activity of superoxide dismutase, catalase and the concentration of malondialdehyde (MDA) in aqueous humor, lens and red blood cells after application of sulfur hexafluoride (SF6) into vitreous of rabbits. MATERIAL AND METHODS: 0.5 ml of 100% SF6 was injected into the vitreous of 24 rabbits of New Zealand race. The animals were randomly divided into 3 groups (of 8 rabbits each) depending on the observation day: group 1-2nd day of experiment, group 2-7th day and group 3-14th observation day. The control group (gr. 0) consisted of 6 rabbits that did not undergo any operations. Activity of superoxide dismutase, catalase and MDA concentration were determined in aqueous humor, lens and systemic blood erythrocytes. RESULTS: On the 7th day of observation an increased activity of dismutase and catalase as well as simultaneous increased MDA concentration were observed. In the lens on the 7th day the increased activity of dismutase was significant in relation to the results in the next time interval, whereas MDA concentration was significantly lower in all time intervals of the experiment in comparison with control group. In erythrocytes an increased activity of catalase was noticed on the 2nd and 14th day. CONCLUSIONS: Increased occurrence of active oxygen species in aqueous humor leads to insufficiency of the antioxidant system and intensification of peroxidation processes, which is reflected by increased MDA concentration. However, in the lens of this experimental model a slight stimulation of antioxidant system by a small number of free radicals is observed, which provokes a reaction of sweeping them away. Efficiency of lens antioxidant system is secured by weakening of peroxidation processes, which is expressed in minimal drop of MDA concentration.

Animals↗

PubMed1997

Nitric oxide release in the peripheral blood during hemodialysis.

These studies evaluated the nitric oxide (NO) release in peripheral blood during a four-hour hemodialysis (HD) with single-used cuprophane (CU), polysulfone (PS) and polyacrylonitrile (PAN) membranes in 10 chronic uremic patients. Continuous monitoring of blood NO concentrations was performed with a sterile NO sensor probe inserted vertically into the arterial blood line between the arteriovenous fistula and dialyzer. In the initial period of HD two peaks of blood NO concentrations were observed: the first occurred at the very start of HD and lasted approximately one minute, and the second peaked to a lesser extent at 20 to 26 minutes after the initiation of HD. The extent of NO release was dependent on the type of dialysis membrane used. Areas under curves for blood NO concentrations (in mumol x min) were as follows: CU, 450.8 +/- 163.3; PS, 247.3 +/- 150.6*; PAN, 200.4 +/- 91.0* (*P < 0.05 vs. CU). During the first hour of HD (N = 6) blood NO concentrations were significantly higher at the outlet of CU dialyzer than those found at the inlet. The areas under their curves (in mumol x min) were 169.1 +/- 1.9 and 107.5 +/- 1.6, respectively (P < 0.001). Areas under curves for blood NO concentrations measured for five minutes following a five-minute in vitro incubation of 5 ml heparinized uremic blood samples (N = 10) with dialysis membranes (50 cm2) were as follows (in nmol x min): CU, 2380 +/- 289*; PS, 1293 +/- 45*; PAN, 1117 +/- 37*; control, 502 +/- 56 (*P < 0.05 vs. control). The addition of sodium heparin to uremic blood platelet suspension induced an immediate rise in NO release in a dose-dependent manner, which proved to be a hyperbolic relationship. During HD with CU (N = 6), PS (N = 6) and PAN (N = 6) membranes blood plasma cGMP concentrations significantly increased, particularly at 20 and 60 minutes of the procedure. No significant differences in blood plasma cGMP levels were found between individual dialysis membranes, and no significant correlations were observed between blood plasma cGMP levels and blood NO concentrations. The results indicate that during HD NO is released in the peripheral blood due to blood-membrane and heparin-blood platelet interactions. The extent of intradialytic NO release is dependent on the type of dialysis membrane used (CU > PS approximately PAN).

Adult↗

PubMed1996

Effects of cation interactions on sugar anion conformation in complexes of lactobionate and gluconate with calcium, sodium or potassium.

In the investigated compounds, the tetrahydrated calcium chloride salt of lactobionic acid (Ca2+.-Cl(-).C12H21O12(-).4H2O), potassium lactobionate (K+.-C12H21O12-), sodium lactobionate monohydrate (Na+.-C12H21O12-.H2O) and calcium galactonate hydrate (Ca2+.2C6H11O7(-).5H2O), the cations and hydrogen-bonding systems have a strong influence on the geometries and conformations of the carbohydrate anions.

Anions↗

PubMed1996

Sex and strain differences of acoustic startle reaction development in adolescent albino Wistar and hooded rats.

Acoustic startle responses (ASR) were studied in 12 young Wistar albino and in 15 hooded rats of both genders. The six week old animals were first exposed to a 6.9 kHz tone pair of 2 ms pulses of 120 db intensity with the inter-stimulus interval (ISI) between 2 and 11 ms. ASR amplitudes and latencies as a function of the ISI, animal strain and gender were recorded and analyzed for ten consecutive weeks. No differences in the ASR amplitude between Wistar and hooded rats were found. ASR amplitude increased during the experimental period and followed body weight increase. Significant differences were also observed between male and female rats in their startle responses to acoustic stimuli. Generally, male subjects responded with a greater ASR amplitude than females, and the changes may be attributed to the difference in neuromuscular development between genders. This experiment sets a background for further developmental studies.

Acoustic Stimulation↗

PubMed1996

[Metabolic changes in blood oxygen in the human heart under ischemic and reperfusion conditions].

The number of granulocytes, their capability to generate O2-. and the activity of SOD-1, GSH-Px, Cat as well as MDA concentrations in erythrocytes in the blood extracted from the venous sinus and aorta under coronary artery bypass with use of St. Thomas cardioplegic solution were determined. The blood for examination was obtained before the institution of cardiopulmonary bypass, in the period of the deepest ischaemia (just after declamping of the aorta) and between the 1-3 minute and the 10-13 minute of reperfusion. A rise in the number of granulocytes both in the venous sinus and aortal blood at all examined intervals was noted. Capability to produce superoxide anion radicals decreased at the peak of ischemia and during reperfusion. The activity of SOD-1 was lower both after the period of ischemia and reperfusion. A rise in aortal blood activity during reperfusion was characteristic for GSH-Px; the activity was greater in the blood sampled from the coronary sinus during ischemia and initial reperfusion. With the exception of the initial reperfusion the activity of Cat diminished in all observed cases. We did not observe any significant changes in MDA concentration with the exception of the initial reperfusion in the aortal blood and later during reperfusion in the blood from the coronary sinus. The results demonstrate that the applied cardioplegic solution may protect myocardium from harmful effects of active oxygen froms produced as a results of ischemia and reperfusion.

Adult↗

PubMed1995

[Generation of active oxygen species by neutrophils in patients with unstable angina pectoris].

The generation of O2-. and H2O2 by neutrophils (PMNs) obtained from peripheral and coronary sinus blood was investigated in twenty four patients with unstable angina, who were qualified for PTCA procedure. The control group consisted of twenty one clinically healthy persons. The blood for the investigations was obtained from coronary sinus and basilic vein just before the procedure, while in the control group from--basilic vein only. In patients with unstable angina statistically significant higher (p < 0.05) O2-. generation by peripheral blood PMNs (at rest and stimulated), compared with the control group, was found respectively (the sick: rest--11,09 +/- 1.92; stimulated--25.48 +/- 5.76 nmol/cell/min.; healthy: rest--7.98 +/- 1.06; stimulated--13.58 +/- 1.19 nmol/cell/min). No significant differences in O2-. generation between PMNs obtained from coronary sinus or peripheral blood were found. No differences in H2O2 generation by rest PMNs obtained from coronary sinus or peripheral blood were found in patients compared with the control group. The generation of H2O2 by PMA stimulated PMNs was higher in the sick without statistical significance.

Adult↗

PubMed1995

[The generation of oxygen free radicals in blood in patients with hypertrophy of paranasal sinuses and larynx].

The evaluated of oxygen specious generation in blood granulocytes of patients with hypertrophy of paranasal sinuses and larynx. There were 33 patients tested (13 with hypertrophy of paranasal sinuses, 10 with hypertrophy of larynx and 10 healthy). The superoxide anion generation of peripheral blood granulocytes described by the rate of the reduced cytochrome C at rest and after stimulation with opsonized zymosan according to Bellavite were measured. There were statistically significant differences between the superoxide anion generation in patients with hypertrophy of paranasal sinuses and larynx in comparison with the healthy ones.

Adult↗

PubMed1994

[Some indices of peripheral blood oxidative metabolism during acetate and bicarbonate hemodialysis in patients with chronic uremia].

Whole blood superoxide anion generation, erythrocyte superoxide dismutase (SOD-1) and catalase activities and erythrocyte and plasma malonyldialdehyde concentrations were evaluated during cuprophane hemodialysis using bicarbonate and acetate dialysate. Superoxide anion generation by resting phagocytes was found to be significantly higher during bicarbonate hemodialysis, while its generation by opsonized zymosan--stimulated phagocytes did not differ between hemodialyses with both kinds of dialysates. Changes in the erythrocytes SOD-1 activities, found in the initial period of hemodialysis, were independent of the kind of dialysate, while erythrocyte catalase activity was higher during acetate hemodialysis in comparison with bicarbonate one. The kind of dialysate did not affect extent of erythrocyte membrane lipid peroxidation, while plasma lipid peroxidation was lower during acetate hemodialysis in comparison with bicarbonate one. In vitro studies with blood cells incubated with acetate or bicarbonate ions in concentrations, which are observed in vivo during hemodialysis, suggest that probably these ions do not directly affect superoxide anions generation, erythrocyte SOD-1 and catalase activities and erythrocyte membrane lipid peroxidation.

Acetates↗