PubMed Health⌕ Search

Biomedical subjects

J Błaszczyk

Publications and source records attributed to J Błaszczyk.

At least 37 records · Page 2Linked to original sources
PubMed1994

[Influence of percutaneous transluminal coronary angioplasty (PTCA) on superoxide anion generation by whole blood granulocytes in patients with unstable angina pectoris (part I)].

In twenty patients with unstable angina, the effects of percutaneous transluminal coronary angioplasty (PTCA) on superoxide anion generation by neutrophils (PMNs) obtained from ulnar vein (UV) and coronary sinus (CS) were investigated. Blood samples for the investigations were taken from CS just before the procedure, within the first minute after the last balloon inflation and 20 minutes after PTCA; from BV--just before the procedure and 20 minutes following it. The results were compared with those obtained from 12 patients with unstable angina, (in whom coronary angiography was performed, but for various reasons angioplasty was desisted from) and with those obtained from 20 clinically healthy persons. In patients with unstable angina, the significantly increased O2.- generation by PMNs from CS and BV was found at rest as well as following opsonized-zymosan stimulation, compared with healthy persons. Within the first minute after PTCA, the significantly decreased O2.- generation by PMNs from CS was observed, compared with the results before the procedure (rest.--11.29 +/- 0.73 and 10.15 +/- 0.65 nmol/cell/min., p < 0.05; stimulation--27.62 +/- 6.25 and 18.54 +/- 3.37 nmol/cell/min., p < 0.05). Twenty minutes after PTCA O2.- generation by PMNs from CS still decreased (rest--9.23 +/- 1.08 nmol/cell/min., stimulation--15.71 +/- 5.98 nmol/cell/min.). In blood samples taken from BV before and after PTCA, no statistically significant differences in O2.- generation were observed (rest--11.32 +/- 0.79 nmol/cell/min. and 10.77 +/- 1.43 nmol/cell/min.; stimulation--25.96 +/- 6.12 nmol/cell/min. and 21.15 +/- 5.47 nmol/cell/min.).

Adult↗
PubMed1991

Whole blood superoxide anion generation and efficiency of some erythrocyte antioxidant systems during recombinant human erythropoietin therapy of uremic anemia.

Six chronic uremic patients on regular hemodialysis treatment were given recombinant human erythropoietin (r-huEPO) in a dose of 50 U/kg of body weight intravenously thrice weekly for 14 weeks. Following r-huEPO therapy, unstimulated whole blood superoxide anion (O2) generation did not change significantly, while opsonized zymosan-stimulated whole blood O2 generation increased. At the same time, erythrocyte superoxide dismutase and, in particular, glutathione peroxidase activities were found to be reducing with concomitant lowering of erythrocyte malonyldialdehyde (MDA) concentrations and increase in plasma MDA concentrations.

Adolescent↗
PubMed1991

[Generation of active oxygen compounds by whole blood granulocytes in patients with chronic renal failure].

In patients with chronic renal failure (crf) on conservative treatment whole blood granulocyte superoxide anion (O2-) generation was significantly decreased. Regular hemodialysis treatment did not induce its significant improvement. As granulocyte O2- generation determines production of other active oxygen compounds, this phenomenon may lead to the impairment of phagocyte bactericidal activity and increased susceptibility to infections in crf. Various methods of determination of active oxygen compounds give incomparable results.

Adolescent↗
PubMed1990

[A case of glomus jugulare chemodectoma].

A case of glomus jugular chemodectoma is described in a 38 years old woman who was treated surgically. In the postoperative course a haemorrhage from the internal carotid artery appeared, needing a second surgical intervention, that was performed with good result.

Adult↗
PubMed1989

Superoxide anion generation, erythrocytes superoxide dismutase activity, and lipid peroxidation during hemoperfusion and hemodialysis in chronic uremic patients.

In 11 chronic uremic patients superoxide anion generation in whole blood, both without and with opsonized zymosan stimulation, was lower than that in 11 healthy controls, while erythrocyte superoxide dismutase (SOD-1) activity and erythrocyte and plasma malonyldialdehyde (MDA) concentrations were elevated. During hemoperfusion (HP) and hemodialysis (HD) superoxide anion generation transiently significantly increased. Changes in the erythrocyte SOD-1 activity and plasma and erythrocyte MDA concentrations during HP suggested that this procedure exerted beneficial effects on lipid peroxidation. On the other hand, during HD erythrocyte membrane lipid peroxidation seemed to be enhanced even further; this phenomenon took place mainly within the dialyzer and a decrease in the erythrocyte SOD-1 activity seemed to be one of the contributing factors. Results of in vitro experiments with cross-incubation of erythrocytes and blood plasma and incubation of whole blood with cuprophan membrane suggest existence of an SOD-1 activator in the uremic blood plasma, which is possibly eliminated during HD.

Adult↗
PubMed1985

Control of locomotion velocity in tetrapods.

Speed control mechanisms were investigated during overground locomotion in the dog. Swing and stance durations as well as stride length were measured for the four limbs simultaneously. Other locomotion parameters such as the mean velocity of the run, phase shifts, step length and the mean velocity of limb transport during the swing phase were calculated on the basis of the measured parameters. It was shown that the increase in stride length was almost linear in relation to the increase in locomotion velocity. There were similar changes in the stepping frequency although changes of this parameter were not strictly linear and depended on changes of step length. During moderate trotting the amplitude of limb movements was almost constant and the velocity of limb transport during the swing phase was minimal. Moreover in the symmetrical gaits spatial phase shifts between unilateral limbs were equal to zero, which means that hind and fore limbs were placed in the same point during successive steps.

Animals↗
PubMed1983

Stepping frequency and stride length in animal locomotion: a new method of investigation.

A new method of investigating animal locomotion is presented which allows for the simultaneous measurement of the basic overground locomotion parameters: swing and stance durations, stride lengths, velocity of movement, gait diagrams and temporal relationships between all animal limbs. The measurements and recordings are given in detail to exemplify the application of the method in dogs.

Animals↗
PubMed1983

Speed control in animal locomotion: transitions between symmetrical and nonsymmetrical gaits in the dog.

Transitions between symmetrical and nonsymmetrical gaits as a speed control mechanism in dog locomotion were investigated. The external locomotion parameters such as swing and stance durations as well as stride length were measured in freely moving animals and were used to determine the spatial and temporal phase shifts between limb movements. The typical transition from trot to gallop and two kinds of gallop-trot transitions are described in detail. Such transitions occur usually in one or two steps and are linked with step length momentary changes within one diagonal pair of limbs. An example of walk to trot transition is also shown. This transition also occurred abruptly in one step and rapid leaps of the phase shift during walk - trot transitions were caused by a momentary decrease of the hindlimb step lengths. Finally, correlation between the external parameters of locomotion and the speed is discussed.

Acceleration↗
PubMed1979

Effect of zinc ions on the oxygen affinity of hemoglobin and the level of 2,3-DPG in normal human red blood cells in vitro.

This paper presents the results of experiments on the in vitro effect of zinc ions on the affinity of hemoglobin for oxygen and on the 2,3-DPG content of the red blood cells of healthy humans. Blood was incubated in 1.5 X 10(-3) M ZnSO4 solution for 4 h and then the oxygen affinity of hemoglobin and the hematocrit number were determined. An original method of determination the oxygen dissociation curve of HbO2 was employed enabling an exact calculation of the P50 value of the hemolysates by the method of least squares. Analysis of statistically verified results reveals that zinc ions increase the oxygen affinity of hemoglobin bringing about a leftward shift of the HbO2 dissociation curve (by 3.85 mm Hg for P50) the level of 2,3-DPG remaining constant in the red cells incubated with ZnSO4. The mechanisms of this phenomenon and its possible clinical implications are discussed.

Cells, Cultured↗
PubMed1978

[Effect of zinc ions on the selected osmotic characteristics of human erythrocytes in vitro (author's transl)].

Effect of zinc on the osmotic fragility-stability of human erythrocytes in hypotonic NaCl solutions and on the reversion of hemolysis was studied in vitro. Erythrocytes washed in 0.9% NaCl were incubated in 1.5 X 10(-3) M ZnSO4 solution in 0.6% NaCl. The obtained results indicate an osmotic stabilization of the erythrocyte membrane by Zn2+ ions increase in osmotic fragility-stability in hypotonic NaCl solutions and increase in reversion of hemolysis due to the effect of Zn2+ ions. As the process of stabilization of the erythrocyte membrane by zinc ions was found to be independent of temperature, a non-enzymatic character of this phenomenon is postulated on this basis and on the basis of literature data.

Erythrocyte Membrane↗
PubMedDate not supplied

Changes in the structure and properties of corpus vitreous and the preservation of enzymatic composition of rabbit antioxidative system under the influence of sulphur hexafluoride gas.

PURPOSE: To evaluate clinical, physiochemical and biochemical changes in rabbit vitreous body caused by local injection of sulphur hexafluoride gas. MATERIAL AND METHODS: The volume of fluid vitreous fraction was measured with Na+, K+, Mg2+, Ca2+ levels and full proteins concentration in both vitreous fractions in 24 New Zealand rabbits at 2, 7 and 14 day after SF6 injection. The activity of superoxide dismutase, catalase and malonyl dialdehyde were used to evaluate the activity of antioxidative enzymatic system. Control group consisting of 6 New Zealand rabbits had no experimental procedures. RESULTS: In the investigated group, the fluid vitreous fraction volume was increased while gelatous one was diminished from 0.08 ml in control group to 0.32 ml in the study group (on day 14). The level of Na+, K+, Mg2+, Ca2+ in the fluid fraction was unchanged. On day 7, we noticed statistically significant increase in protein concentration in comparison with the control group and the study group on 14 day. The activity of superoxide dismutase and catalase as well as the level of malonyl dialdehyde were increased in the fluid vitreous fraction compared to the gelatous one in the control group. After the SF6 injection we did not observe any changes of superoxide dismutase and catalase activities in the gelatous part of vitreous body while in the fluid one there was statistically significant decrease in the enzymatic activity and the MDA level in the whole observation time. CONCLUSIONS: The injected sulphur hexafluoride gas caused the damage of the gelatous vitreous fraction with the increase in the fluid one. The oxygen free radicals might trigger these pathological processes.

Animals↗
PubMedDate not supplied

Moderate systemic hypothermia and cold crystalloid cardioplegia influence on myocardial ischemic and revascularisative injury.

The number of granulocytes, their ability to generate superoxide anion (O2-) and the activities of Cu, Zn--superoxide dismutase (SOD-1), glutathione peroxidase (GSH-Px), catalase (CAT) as well as malonyldialdehyde (MDA) concentrations in erythrocytes in the blood extracted from the venous sinus and aorta under coronary artery bypass were examined with the use of St. Thomas Hospital cardioplegic solution. Specimens at the peak of ischemia of the right atrium for ultrastructural examination of the endothelial cells of capillary vessels and sarcomers were taken. The blood was obtained during cardiopulmonary bypass (CPB) before the aorta clamping and immediately after aorta declamping (peak of ischaemia) between 1-3 minute and 10-13 minute of reperfusion. Increase of the number of granulocytes both in the coronary sinus and aortal blood at all examined intervals as well as decrease in the number of ones in sinus compared with aortal blood was noted. The ability to produce superoxide anion radical decreased at the peak of ischemia and during reperfusion. The activity of SOD-1 was lower both after the period of ischemia and reperfusion. The increase in aortal blood activity during reperfusion was characteristic of GSH-Px; the activity was higher in the blood sample from the coronary sinus taken during ischemia and initial reperfusion. With the exception of the initial reperfusion the activity of CAT diminished in all observed cases. MDA concentration did not demonstrate any significant changes with the exception of the initial reperfusion in the aortal blood and later towards the end of reperfusion in the blood from the coronary sinus. Ultrastructural studies indicated overhydration of the cells both in the endothelium and the intercellular space. The obtained data demonstrate that the applied cardioplegic solution protects the myocardium from harmful effects of reactive oxygen species produced as a result of ischemia and reperfusion.

Adult↗
PubMedDate not supplied

[Production of superoxide free radicals by peripheral blood leukocytes in patients with psoriasis treated by the Re-PUVA-C method].

The investigations were carried out in 20 patients with severe forms of psoriasis, and in 18 healthy subjects. The generation of free superoxide radicals by polymorphonuclear leukocytes of peripheral blood was evaluated by Bellavite et al. (1983) method by use superoxide dismutase of Sigma firm. The obtained results may confirm alterations in PMNL functions in patients with psoriasis observed by many authors. It may confirm the probability of damage of membrane structures and alterations in their bactericidal functions.

Adult↗
PubMedDate not supplied

Adenine nucleotides and 2,3-dophosphoglycerate in the erythrocytes during physical exercise and restitution in healthy subjects.

The reported investigations were carried out in 6 young (20 years old) healthy males who performed a 10-minute exercise on a Monark cycle ergometer at workloads increasing the heart rate to 170/minute. Before the exercise, after its termination, and after 30 minutes of restitution the concentrations of adenosine-5' triphosphate, adenosine-5' diphosphate, adenosine-5' monophosphate, 2,3-diphosphoglycerate, nicotinamide-adenine dinucleotide and nicotinamide-adenine dinucleotide phosphate were assayed in erythrocytes. After the end of exercise a non-significant increase in ATP concentration and a non-significant decrease of 2,3-DPG were found in the erythrocytes. The concentrations of ADP and AMP were lower than at rest, both, after the end of exercise and after 30 minutes of restitution (p less than 0.05). The concentrations of nicotinamide-adenine dinucleotide and nicotinamide-adenine dinucleotide phosphate decreased after exercise (p less than 0.05) fell even more after 30 minutes of restitution (p less than 0.05).

Adenine Nucleotides↗