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Biomedical subjects

J Bouda

Publications and source records attributed to J Bouda.

At least 37 records · Page 2Linked to original sources
PubMed1986

[The effect of the use of milk-based feed mixtures and whole milk on the development of selected indicators in the blood plasma in calves].

Two milk feeding systems were investigated as influencing the health and development of calves. After the termination of colostrum feeding, the ten animals of the experimental group were given whole milk whereas the control group (also ten calves) was given the Laktosan milk replacer. By the age of three months, blood was collected from the calves for biochemical examination in weekly intervals, later once a month. The content of urea, determined in the blood plasma of the calves of the experimental group was significantly lower in the fourth to seventh week. The plasma levels of nitrogen, potassium, calcium and magnesium were about the same in the experimental and control groups, being within the limits of the reference values. At the age of six to nine weeks, the content of inorganic phosphorus in the blood plasma of the tested animals was statistically significantly higher. Vitamin A concentration in the blood plasma was about the same in both groups. The content of vitamin E in the blood plasma of the calves of the experimental group was statistically significantly higher in the fourth to eight week of age. No significant differences between the two groups were observed in the plasmatic activities of aspartate aminotransferase (AST) and gamma-glutamyl transferase (GMT). The activities of alkaline phosphatase (ALP) were significantly higher in the third to fifth week of life. From the fifth to eighteenth week of age, the average daily weight gains were significantly higher in the calves given whole milk.

Age Factors↗
PubMed1986

[Utilization of urea nitrogen in young calves].

The influence of an increasing intake of urea in feed rations for calves on its levels in blood plasma and urine, development of health condition, weight gains and on other biochemical characteristics in the blood of calves was studied. The experimental group (n = 4) received urea in feed ration from the 17th day of age, at the beginning in the preparation Syrur, later on (from the 66th day) in the synthetic form only. The feed ration of the control group (n = 3) differed only in the zero content of urea. Over the period of ten weeks the blood was sampled for biochemical examination twice a week, since the 52nd day of age urine was also taken at the same intervals. Starting the 24th day of age, the levels of urea in the blood plasma of experimental animals were significantly higher (P less than 0.05). The concentrations of urea in the urine of the animals belonging to the experimental group were also higher, however, the differences in the average values were not significant. In both groups of animals under study no significant differences in the content of vitamin A, concentration of total protein, glucose, bilirubin, activities of aspartate aminotransferase (AST), gammaglutamyl transferase (GMT) and lactate dehydrogenase (LD) were observed. The average daily weight gains were higher in the calves of the control group, however, the differences were not significant. Over the whole experimental period the health condition of both groups of calves was good.

Age Factors↗
PubMed1985

[Selected indicators of colostrum quality in cows over the period of a year].

Under the conditions of large-scale breeding, seasonal changes in the total protein of colostrum whey, gammaglobulins, vitamins A, E, C, and carotene in cow's colostrum from the first milking, were studied in the course of the first 12 months. As indicated by the results of an analysis of 302 colostrum samples, the concentrations of total protein in whey were significantly higher in winter (P less than 0.01) than in the summer season. On the other hand, vitamin C levels were higher in summer than in winter. A lower level of total protein of colostral serum than the required minimum of 120 g per litre was recorded in 64.4% of the cows. A highly positive correlation (r = +0.981) was demonstrated between the concentration of total protein and gammaglobulins in colostrum whey. This fact makes it possible to consider and use for diagnostic purposes the total protein of whey as a sufficiently exact parameter of colostrum quality without having to determine the gammaglobulin level. On the basis of these analyses it is possible to some extent not only to evaluate the health condition of high-pregnant cows and the quality of attendance, but also to prognosticate the health of their calves.

Animals↗
PubMed1982

[The effect of colostral immunoglobulins on hematological and biochemical indicators in calves].

Haematological values (haemoglobin concentration, haematocrit, erythrocyte and leucocyte number) and the levels of biochemical parameters (plasma concentrations of magnesium, zinc and copper) were studied in dependence on the level of immunoglobulins in calves in the early postnatal period. As a parameter of colostrum intake and quality the immunoglobulin level in blood serum was chosen, according to which the calves (n = 300), aged from two to six days, were divided into four groups. A relationship between the colostrum intake, or the immunoglobulin level in blood serum, and the magnesium concentration in blood plasma was observed. The magnesium level in plasma was in correlation with the Ig level in the blood serum of calves. As far as the other studied blood parameters are concerned, no statistically significant differences were proved in the calves aged two to six days.

Animals↗
PubMed1981

[Comparison of biochemical parameters in the blood of healthy and diseased calves in a large barn].

In a high-capacity calf-house, twenty-one calves were subjected to repeated clinical and biochemical examination until they were 22 weeks old. For evaluating the health condition of the animals, the calves were divided into two groups, one including healthy animals and the other including those suffering from bronchopneumonia and diarrhoea as the most frequent disorders in high-capacity calf-houses. From four to eight weeks of age, both groups of calves showed low levels of plasma magnesium and iron. The levels of plasma zinc and copper showed a continuous decrease with age. Compared with the healthy calves, the animals affected by a disease had statistically significantly lower levels of plasma vitamins (A, E, C). Further, the diseased animals also showed statistically significantly decreased levels of plasma glucose, serum albumins, and a lower activity of alkaline phosphatase in plasma. In addition to this, the diseased animals also had significantly higher levels of plasma gamma-globulins and urea. These clinico-biochemical parameters can be used for determining the subclinical forms of disease, for objective diagnosis and for the introduction of preventive measures in high-capacity calf-house.

Animals↗
PubMed1980

[The effect of Rumensin on the health status and energy metabolism of feedlot cattle].

The effect was studied of sodium monensinate dosed 125 mg pro die and given to feeder cattle on the health condition and some clinico-biochemical parameters in view of the energy metabolism. The experiment was performed under current farming conditions in a pavilion fattening house. The experiment, which lasted 367 days, was conducted with 985 bulls of the Bohemian Spotted breed. Ten animals from the experimental and ten animals from the control pavilion were subjected to detailed clinical and clinico-biochemical examination. The feed ration was based on corn silage with an additive of dried poultry litter and concentrates. The additive was administered in the concentrate mixture, 125 mg per head/day. The health condition of the bulls was good during the whole period of study, and except several cases where neck chain wounds were observed, or small traumatic alterations on the extremities, no health disorders occurred. Some clinico-biochemical parameters of the blood were followed to study the metabolic processes and health condition. pH, pCO2, BE, SB were studied to find out the acid-base relations of the blood. As to the energy metabolism, the levels of glucose, total protein, urea, nonesterified fatty acids, triglycerides, cholesterol and total lipids were followed. Among other hematologic parameters studied there were hemoglobin, hematocrit, beta-carotene and the levels of glutamate-oxalacetate transaminase and glutamyl-transpeptidase in relation to liver metabolism. During the period of study, increased urea levels were found in the experimental animals, and on the other hand lower levels of free fatty acids and triglycerides in the blood serum. No significant changes in the other studied parameters were observed in the course of the experiment. It can be stated according to the obtained results that sodium monensinate did not influence negatively the studied clinico-biochemical parameters nor the health condition.

Animals↗