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Biomedical subjects

J C Meyer

Publications and source records attributed to J C Meyer.

60 records · Page 4Linked to original sources
PubMed1977

[Different behavior of human melanoma cells from primary tumor and metastases in vitro].

The morphologic differences of two melanoma cell cultures from a primary melanoma and a metastasis are described. Though both cultures looked very similar at the beginning, they developed into totally different forms. Long, thin spindle-shaped cells containing an optically dense plasma grew in the cultured from the primary melanoma during its whole life span and the epithelioid cells of metastasis culture separated itself into different subcultures. The cells of both subcultures detached from the bottom of the culture flasks after 15 days of growth and began to spread again 7 days later with two very different forms. This transformation was observed three more times at monthly intervals. The possible relationship between these morphological alterations and the ability of the melanoma cells to produce metastases as well as the usefulness of melanoma cell cultures for immunodiagnostic purposes are discussed.

Cells, Cultured↗
PubMed1976

Spectrofluorometric determination of bufuralol in blood and urine.

The benzofuran analog bufuralol, a beta-adrenergic blocker, was determined in blood and urine by a specific and sensitive spectrofluorometric assay. The compound was extracted into ether from blood or urine adjusted to pH 10. The ether extract was separated by TLC to resolve the parent drug from any basic metabolites present, and the spots were eluted off the silica gel and quantitated fluorometrically in 0.1 N HCl. The overall recovery of the assay was 85 +/- 3.0%; the sensitivity limit was 2-4 ng/ml of blood or urine, using a 2.5-ml specimen/analysis. The method was applied to the determination of blood levels in a dog following a single 10-mg/kg oral dose and in two human subjects administered a single 20-mg oral dose.

Adrenergic beta-Antagonists↗
PubMedDate not supplied

Pancytopenia secondary to lymphoid leukemia in three horses.

Pancytopenia was observed in two 3-year-old geldings and one 11-year-old mare. All horses had a brief history (2 days to 4 weeks) of fever, anorexia, and depression. One of the three horses had blast cells present on a peripheral blood smear. Examination of the bone marrow showed substantial infiltration with neoplastic lymphoid cells. At necropsy, neoplastic cells were restricted to the bone marrow in one horse, present in bone marrow, liver, and spleen in the second horse, and reported in multiple tissues in the third horse, including bone marrow, kidneys, lung, myocardium and lymph nodes. The value of a bone marrow aspirate and core biopsy in the investigation of pancytopenia is highlighted.

Animals↗
PubMedDate not supplied

Reverse phase liquid chromatographic assay for calcium pantothenate in multivitamin preparations and raw materials.

A reverse phase liquid chromatographic (LC) method has been developed for the assay of calcium pantothenate in commercial multivitamin tablet formulations and raw materials. The assay was validated according to the Pharmaceutical Manufacturers Association Quality Control HPLC Committee guidelines. The chromatographic system includes a C-18 column and a mobile phase consisting of 0.25M sodium phosphate buffer, pH 2.5, and acetonitrile (97 + 3 v/v). The column effluent is monitored by UV detection at 205 nm. The sample preparation involves only extraction in water followed by filtration. The method is stability-indicating with a detection limit of approximately 50 ng/mL of the calcium pantothenate in the samples. The system is linear from at least 0.02 to 0.10 mg/mL. The mean recovery of spiked placebos ranged from 98.7 to 99.8%. The within-day precision of the assay on finished products (N = 6) ranged from 0.3 to 2.0% CV. A system suitability criterion for resolution is based on the separation between calcium pantothenate and 2 closely eluting compounds, saccharin and a saccharin degradation product, 2-sulfamoylbenzoic acid.

Chromatography, Liquid↗
PubMedDate not supplied

Precipitating antibodies in sera of normal blood donors against some antigens related to type III allergy, detected by counterimmunoelectrophoresis.

Precipitating antibodies against five antigens, staphylococcus aureus, candida albicans, cotton wool dust, house dust and tetanus toxoid, related to type III allergy (Arthus reaction) were estimated in 100 sera of normal blood donors by the counterimmunoelectrophoresis technique. The three antigens staphylococcus aureus, candida and cotton wool dust exhibited rather high titres of precipitins in sera of normal blood donors, whereas nearly 80% of the sera yielded no precipitins against house dust and tetanus toxoid. For diagnostic purposes the borderline titres were estimated as follows: 1:8 for staphylococcus aureus, 1:16 for candida albicans and 1:8 for cotton wool dust. Even low titres with the house dust antigen indicated a pathogenic situation.

Allergens↗