PubMed Health⌕ Search

Biomedical subjects

J F David

Publications and source records attributed to J F David.

At least 37 records · Page 2Linked to original sources
PubMed1982

[Hormone receptors of target cells of estrogens and relation to cell growth].

Three carcinomatous cell lines have been established in our laboratory and well characterized: two from endometrial carcinoma (MOQ and GUS), one from a pleural effusion of a breast cancer (FAM). These three cell lines have estrogen (ER) and progesterone receptors (PR). A subline of MOQ (MOQ-2) has now lost its receptors and is R-. With this material some paradoxical results have been observed:--estrogens, at physiological concentrations in the culture medium, do not enhance cell proliferation; estrogens are only active in inducing new PR synthesis; but these R+ cells grow in vitro irrespective of the presence of estrogens;--when these cultivated cells are inoculated into nude mice, only FAM (breast cancer cells) grow spontaneously and give a tumor in some animals; MOQ and GUS (endometrial carcinoma) grow only in 20 to 40% of pre-irradiated nude mice; when estrogen-treated, 100% of the mice have a tumour with MOQ without irradiation, 80% a direct tumour with GUS or FAM. This shows that the same cells which are estrogen insensitive in vitro, are estrogen dependent in vivo;--more surprising is the fact that the MOQ-2 cells (ER-) are the most hormono-dependent ones in nude mice and always give rise to a node in mice supplemented with estrogens (100%). These paradoxical results show that, if we know how the estrogens induce protein synthesis in the cell by the ER system, there is still a gap in our understanding of growth promoting activity.

Animals↗
PubMed1982

[Variation in the number of estrogen and progesterone receptors in normal human endometrial cells in culture].

As estrogens are in vivo potent growth factors of their target cells but are ineffective in vitro on human endometrial cell multiplication, an eventual role of steroïd sex hormone receptors has been suspected. These receptors have been assayed in human endometrium, and cytoplasmic concentrations compared between fresh tissue, short term and long term cultures. Receptors remain present in culture but at a lower level than in vivo. Estrogen receptors keep stable with the duration of the culture while progesterone receptors progressively diminish. More, these receptors are active: estradiol administration induces a new progesterone receptor synthesis in culture. So, steroïd sex hormone receptors do not seem to be involved in the ineffectiveness of estrogens on cell multiplication in culture.

Adult↗
PubMed1981

[Neoplasm-resistance of guinea pigs: demonstration of a principle ensuring destruction of human cancer cells in culture].

The thymus and the spleen of guinea Pigs treated with oestrogen were rich in cells showing a special inclusion, the Foa-Kurloff body cell (F.K.). Thymic, spleen and renal cellular extracts were obtained by tissue crushing and ultra-centrifugation from healthy or oestrogen treated animals. The extracts were added to carcinomatous (HeLa) or to non-carcinomatous cells (MRC5 fibroblasts or foetal human lung cells). The thymic extracts in the non-treated animals and the splenic extracts rich in F.K. body cells induced an inhibition in the growth of carcinomatous cells. These effects were increased with the thymus extracts rich in F.K. bodies. The thymic inclusion of F.K. seems therefore to concentrate tumorous thymic fractions.

Animals↗
PubMed1981

[Chromosomes of normal human endometrial cells in long-term culture].

The number of chromosomes was established in five long-term human normal endometrial cultures, at different times from 15 to 350 days. Among these cultures three were fibroblastic and two epithelial. -- Cultivated fibroblastic cells are essentially diploïd; epithelial cells are frequently polyploïd. -- Epithelial cells become quickly polyploïd but the rate of polyploïdy does not increase with the life span of the culture; so it is not a degenerative mark, but a physiological property of that type of cells, useful for characterization in culture between epithelial and stromal cell lines of the endometrium.

Cells, Cultured↗
PubMed1981

[Cloacogenic transitional epithelium of the anal canal and Solère's theory on cloacal partitioning].

A transitional zone, between the squamous and columnar epithelium is described at the anorectal junction. Its existence, unknown in most of the Histology text books is an indirect evidence of the theory of SOLERE (1969). According to this author, there is a flattened sagittal entoblastic bud, shared by uro-genital and anal zones, achieving the closure of the cloacal cavity in its distal part. Some data which agree with this theory are discussed.

Anal Canal↗
PubMed1980

[Normal human endometrial cells in culture: absence of direct effect of estrogens on cell multiplication].

Primary monolayer cultures of human normal endometrium were realised from 65 wombs of non menopausal women at time of hysterectomy. From these cultures, 19 cell lines were subcultivated in long term cultures (up to 500 days). Primary cultures exhibited a mixture of stromal and epithelial cells. Cell lines were pure : 16 stromal and 3 epithelial lines. These primary, medium-term and long-term cultures were assessed 40 times for growth stimulation by added estrogens. In various conditions of culture medium, delay and duration of administration, estrogens never induced a significant modification of cell proliferation. These results suggest that the in vivo growth-promoting effect of estrogens is indirect, likely mediated by other growth factors.

Cell Division↗
PubMed1979

[Action of thymic extracts on the growth of different cell populations in vitro].

Thymic, splenic and renal cellular extracts were obtained by tissular grinding and ultra centrifugation, from animals having undergone diverse hormonal treatments. These extracts were added to different cellular populations in culture (carcinomatous HeLa strain, established MRCs strain, primo-culture of human foetal lung strain). The thymic extracts of non treated animals and the splenic extracts rich in FK substance from intact guinea pigs treated by oestrogenes or FSH, provoque an inhibition of tumoral cell growth. The most important effects are obtained with thymus extracts rich in FK substance. The FK substance seems capable of concentrating certain antitumoral thymic fractions.

Animals↗
PubMed1979

[Thyroid trabecular carcinoma. A clinicopathological entity of poor prognosis? (author's transl)].

The records of 32 patients with trabecular carcinomas of the thyroid gland were critically reviewed from a previously published serie of 138 thyroid cancers referred to the Centre Claudius Regaud, between 1952 and 1973. On the basis of clinico-pathological considerations, it seems possible to divide trabecular carcinomas into two groups. Pure trabecular carcinomas (moderately differenciated follicular carcinomas--WOH) which have a poor prognosis (5 years actuarial survival: 13%) related to high rate of local recurrences, fast metastatic spread to the lung, bad response to suppressive hormonotherapy and lack of 131 iode uptake by malignant tissue. Mixed trabeculo-vesicular carcinomas which have in comparison a fairly good prognosis (5 years actuarial survival: 63%) in keeping with a lower tendency to local recurrences and a useful concentration of radioactive iodine by metastases (most of them located in the skeleton) although dependent on the pourcentage of vesicles in the tumor process. Among differentiated thyroid carcinomas, distinction between pure trabecular and mixed trabeculo-vesicular carcinomas with quantitative determination of vesicules seems of great interest in relation to the therapeutic approach.

Adenocarcinoma↗
PubMed1979

[Growth and morphologic characteristics of normal human endometrial cells in primary culture].

Monolayer cultures of endometrium were established from 59 normal women after hysterectomy. The technical procedure is described, allowing cells in monolayer to grow satisfactorily for more than 60 days, showing a doubling-time of about 4-5 days. During early passages, two cell populations are characterized by microscopic examination : confluent areas of epithelial polygonal cells and scattered fusiform fibroblastic cells. After the 8th passage, epithelial cells are no longer recognized : the monolayer is homogeneous and all cells are fibroblast-like. These cells are an interesting model system for steroid sex hormone studies.

Cell Division↗
PubMed1979

[Remnants of the thyroglossal duct. A histological study].

This study was undertaken in order to find out the frequency of thyroglossal duct remnants and to specify the relationship between this tract the hyoïd bone. The remnants having undergone cystic pathology refer to tracks edged with an epithelial lining which can be various, associated or isolated, types. Thyroïd follicles may be annexed to this track, in or out of its cystic part. The systematic study of 23 hyoïdal and sub hyoïdal regions of adults exempt of any pathologic remnants allows one to find tracks in 13 cases, that is in every other case. This frequency is higher than the one normally described. These remnants always refer to some thyroïd follicle islands and rarely to an epithelial track. Furthermore, we can sometimes observe the frankly intra hyoïdal situation of the thyroid tissue. This last location is discussed according to embryologic data.

Adolescent↗
PubMed1978

[Some experimental evidences for immune functions of interstitial plasma-cells of the submaxillary glands (author's transl)].

Interstitial plasma-like cells, constantly present in the submaxillary glands, are they endocrine cells or true plasma-cells? They do not show any change in case of hypertrophy of the salivary glands which is observed under isoproterenol repeated administration. In contrary their number increase greatly during the immunologic stimulation by human albumin injections in the Hamster cheek pouches. So, it does appear that they would be true plasma cells involved in immunoglobulins synthesis.

Animals↗
PubMed1977

[Kinetics of I-131 fibrinogen in cancer patients: pharmacological approach].

The results obtained in a previous study using 131I fibrinogen in cancerous patients suggested a local intravascular clotting precess. In order to elucidate the mechanism of fibrinogen kinetic abnormalities different drugs, including heparin, prednisone, ticlopidin, aspirin and indomethacin were administered in 68 patients and their effects evaluated by change in the 311I fibrinogen disappearance rate. The results suggest that these drugs may counteract with the early stages of coagulation (kinin-forming system, factor XII) and that abnormal 131I fibrinogen kinetic in cancer would be a non specific phenomenon.

Aspirin↗
PubMed1977

[Thyroid carcinomas. Critical study about 138 observations. Therapeutic deductions (author's transl)].

Between 1952 and 1973, 138 patients with thyroïd carcinoma have been recorded. From the critical study of these cases, the pathological, etiological and clinical features have been studied. Different therapeutic modalities are evaluated from these cases. Surgery was always used when possible. A hemithyroïdectomy was realised most often. We stress that sterilisation was obtained by 131I treatment in plurifocal metastasis, particularly in pulmonary metastasis, only when the iodine uptake was sufficient. Survival has been evaluated according to the pathology: papillary and vesicular carcinomas have a good prognosis (the papillary and vesicular survival rate at 10 years reaches respectively: 65% and 64%). Anaplastic carcinomas have a bad prognosis (the survival rate at 5 years is 18%, at 10 years, 0%. These results are similar to those published by differents authors. At the opposite, trabecular carcinomas have a very short survival (8% at 5 years, 7% at 10 years) which is very unusual in well differentiated thyroïd carcinomas. It has to be isolated from the others.

Age Factors↗