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Biomedical subjects

J Kellner

Publications and source records attributed to J Kellner.

10 recordsLinked to original sources
PubMed1992

Development of a direct and indirect chemiluminescence immunoassay for the detection of an organophosphorus compound.

The following study describes the development of two immunoassays for the semiquantitative determination of the organophosphorus compound methyl phosphonic acid, p-aminophenyl 1,2,2-trimethyl-propyl diester (MATP). These assays are a direct competitive, labelled-hapten, enzyme immunoassay and a competitive enzyme immunoassay with indirectly labelled antibody, both with luminescence determination of peroxidase. A camera luminometer was used to determine final signal strength. A constant light reaction was reached using luminol (5-amino-2,3-dihydro-1,4-phalazinedione), and by adding coumaric acid as an enhancer. A five minute interval was selected as exposure time. This phototechnical system makes a semiquantitative detection of MATP possible. The detection limits for both assays were at 10(-6)mol/l MATP. With the help of a microtiter luminometer (reader) the detection limit was reduced to 5 x 10(-8) mol/l MATP, by measuring the real intensity of the chemiluminescence signal.

Antibodies, Monoclonal↗
PubMed1992

The influence of various adjuvants on antibody synthesis following immunization with an hapten.

For the production of specific antibodies to the hapten MATP (4-Amino-1,2,2-trimethyl-phenylphosphonate) in Balb/c mice various non-toxic adjuvants were compared to Freund's complete adjuvant (FCA). For immunization the hapten MATP was coupled to the carrier human serum albumin (HSA). The immunostimulating effect of the synthetic lipopeptides Pam3Cys-OH, Pam3Cys-Ser-Ser-Asn-Ala and different concentrations of the lipohexapeptide Pam3Cys-Ser-(Lys)4 (Pam3Cys = S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-N- palmitoyl-(R)-cysteine as well as of aluminium hydroxide were tested. IgG antibody titers in serum were determined in ELISA. In dose-response studies 50 micrograms Pam3Cys-Ser-(Lys)4 per mouse was the most effective dose with a long period of high antibody levels after the second booster. Pam3Cys-Ser-Ser-Asn-Ala provoked only low antibody titers. Immunostimulation with Pam3Cys--OH did not result in an increased production of specific antibodies. Compared to the control group an enhanced antibody synthesis could be provoked with aluminium hydroxide. However, the increase was much smaller than by using FCA. The lipopeptide Pam3Cys-Ser-(Lys)4 turned out to be a very potent adjuvant. One week after booster injection into mice 50 micrograms of this substance helped to elicit a higher antibody titer than FCA. Hence, as far as the degree of antibody production is concerned, Pam3Cys-Ser--(Lys)4 represents an alternative adjuvant to FCA.

Adjuvants, Immunologic↗
PubMed1991

[Staging of rectal carcinoma by endorectal ultrasonography].

Staging of rectal carcinoma is essential for its management. 23 patients with rectal lesions were examined by endorectal ultrasonography. Of these 19 had rectal carcinoma and in 4 there was an abscess of the rectal wall, previously diagnosed by sigmoidoscopic biopsy. 10 of those with carcinoma were examined by computerized tomography as well, and 10 were operated on. In most cases the staging of rectal carcinoma by endorectal ultrasonography was accurate and compared well with the operative histological results. We conclude that endorectal ultrasonography is the method of choice for staging of rectal carcinoma prior to treatment.

Carcinoma↗
PubMed1991

Influence of various adjuvants on the synthesis of specific antibodies of chicken, sheep and rabbit following immunization with an hapten.

In order to obtain high amounts of specific antibodies against the hapten methylphosphoric acid, para-aminophenyl-1,2,2-trimethyl-propyldiester (MATP) different animals were immunized with MATP coupled to the carrier protein human serum albumin (MATP12-HSA) using several adjuvants. The best specific immune response in sheep, rabbit and chicken was reached with Freund's complete adjuvant with animal specific differences being tested by an ELISA. The adjuvants aluminium hydroxide (5% and 10%) and diphosphoryl lipid A showed no significant difference compared to the control group (NaCl with MATP12-HSA). In rabbits and chickens MATP12-HSA can be used to reach an immune response without the help of an adjuvant.

Adjuvants, Immunologic↗
PubMed1987

[Pressure measurements of the round window membrane in the guinea pig cochlea].

Some clinical observations suggest that sudden hearing loss or dizziness can be due to rupture of the round window membrane. Experiments on 16 guinea pigs showed that after injection of fluid the pressure in the scala tympani of the basal turn increases. This leads to rupture of the round window membrane. The pressure was recorded via a piezoresistant transducer. Morphological changes were visualised by scanning electron microscopy.

Animals↗
PubMed1985

Ectopic prostatic tissue in urethra.

Ectopic prostatic tissue in the urethra is an uncommon lesion. It appears more often in young males. The common presenting symptoms are hematuria or hemospermia. It is a benign lesion, and transurethral fulguration is believed to be adequate treatment.

Choristoma↗
PubMed1975

A model for differential treatment of the juvenile delinquent.

In this model, the symptom neurosis is contrasted with the ego syntonic deviance. Whereas most of psychotherapeutic practices are geared to neurotic patients, this model represents treatment procedures and techniques aimed at the acting-out character disorders. This comparison related to: a) internal tension and readiness for change b) level of differentiation between Actual and Potential Identity c) perception of the Logic of Events Furthermore, the model describes the ego-defenses of the delinquent, and finally relates them to the more specific techniques of treatment.

Acting Out↗