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Biomedical subjects

J Klatka

Publications and source records attributed to J Klatka.

At least 19 recordsLinked to original sources
PubMed2006

Differentiation between cholesteatoma and inflammatory process of the middle ear, based on contrast-enhanced computed tomography imaging.

OBJECTIVE: To assess the usefulness of delayed post-contrast computed tomography (CT) examination for the detection of residual or recurrent cholesteatoma after canal wall up tympanoplasty. STUDY DESIGN AND SETTING: This prospective, non-randomized study, set within an academic medical centre, included 17 consecutive patients who had undergone canal wall up tympanoplasty for cholesteatoma, with possible recurrence. Pre-contrast CT scans and delayed post-contrast images were compared with second look surgical findings. RESULTS: A residual or recurrent cholesteatoma was found in eight of the 17 patients at revision surgery and was correctly diagnosed on post-contrast CT images in six patients (75 per cent). In the two misdiagnosed cases, cholesteatoma pearls smaller than 2.5 mm were not seen on post-contrast CT. The sensitivity of the imaging test was 75 per cent, the specificity was 60.1 per cent, the positive predictive value was 88.1 per cent and the negative predictive value was 81.8 per cent. CONCLUSION: Computed tomography with delayed post-contrast images is a sensitive imaging modality for the detection of residual cholesteatoma. If proven reliable, this method of non-invasive imaging could spare the patient unnecessary revision surgery.

Adult↗
PubMed2004

Loss of heterozygosity in laryngeal cancer.

PURPOSE: Head and neck cancers account for about 6% of all human cancers. Molecular changes leading to the disease development and progression still remain not fully explained. Examination of loss of heterozygosity (allelic loss, LOH) using the specific microsatellite markers is a method of choice in assessing tumour suppressor genes (TSGs) localisation in human genome. MATERIAL AND METHODS: The study was performed in a group of 46 male patients, aged 42-77 years. Forty three patients underwent total laryngectomy with lymph nodectomy, two patients--chordectomy and one patient--partial laryngectomy. Tumour tissue specimens and reference peripheral blood samples were obtained during surgical resections. Standard methods were used for DNA isolation. Fluorescent multiplex PCR was used to amplify microsatellite loci included in commercially available human identification kits. RESULTS: LOH was found at the following loci: BAT26, D3S1358, FGA, CSF1PO, D5S818, D8S1179, VWA, D13S317, D18S51. The highest LOH frequency was found in the tumor samples where the neighbouring cervical lymph nodes were affected but the incidence of LOH at BAT26 was statistically insignificant (p = 0.07). CONCLUSIONS: High incidence of LOH is considered an unfavourable prognostic factor accompanying an aggressive nature of the tumour and indicating an involvement of certain genome regions in cancerogenesis. In head and neck cancers LOH was found on the following chromosomes: 3p, 5q, 8p, 9p, 9q, 11q, 17p, 17q, 18p, 18q.

Adult↗
PubMed2002

Establishment and preliminary characterization of two cell lines derived from larynx carcinoma.

Two new cell lines, designated as RK-33 and RK-45, have been successfully established by an outgrowth technique from two different larynx tumours obtained from patients after laryngectomy. Both cell lineshave been maintained incultureforover 18 monthsandrecently have reached passage number 220 (RK-33) and 110 (RK-45). The cells display an epithelial morphology and multiply with a population doubling time of about 24 h (RK-33) and about 40 h (RK-45). The epithelial nature of the cells was also confirmed by expression of cytokeratins 8 and 18. Both lines were sensitive to antiproliferative effect of the tested cytostatic agents such as methotrexate. etoposide and thiotepa, with methotrexate being the most effective. We believe that both cell lines: RK-33 and RK-45 could be a suitable model for studying larynx cancer biology, however, further characterization of their properties is needed.

Antineoplastic Agents↗
PubMed2001

Prognostic value of the expression of p53 and bcl-2 in patients with laryngeal carcinoma.

Previous studies have yielded inconsistent results on the prognostic significance of p53 and bcl-2 in head and neck cancer. The aim of this study was the evaluation of p53 and bcl-2 protein expression in laryngeal squamous cell carcinoma and to clarify the relationship between them. All patients with laryngeal carcinoma were treated during the period 1991-1993. In the present study p53 and bcl-2 expression in paraffin sections from 50 cases of laryngeal squamous cell carcinoma were analysed and correlated with routine clinico-pathological parameters. The expressions of p53 and bcl-2 were examined by immunohistochemical staining. Immunoreactivity for p53 was observed in 45 (90%) of carcinomas and bcl-2 immunoreactivity in 7 (14%). No significant correlation between the p53 or bcl-2 expression and patients' T- or N-stage, histological grading, or overall survival was found.

Adult↗
PubMed2001

[Epistaxis: causes and management].

Evaluation of 265 patients treated in hospital due to nasal bleeding revealed that almost half of them suffered from hypertension and in about 30% of cases it was impossible to establish the cause of epistaxis. The most frequent way to stop the bleeding was anterior nasal packing and in case of failure posterior nasal packing together with anterior one. Posterior nasal packing with Foley's catheter is relatively simple and effective procedure. Introduction of superselective embolization of maxillary artery and dermoplasty in Rendu-Osler disease was very helpful.

Adolescent↗
PubMed2001

[Melanoma of nasal cavity and paranasal sinus mucosa].

In a group of 15 patients with malignant melanoma of the nasal cavity and paranasal sinuses the place of origin was nasal mucosa in 11 patients, the ethmoid cells in 2 cases and the maxillary sinus in 2 cases. Only 5 patients had tumour limited to infrastructure of the nasomaxillary complex, in 10 patients tumour occupied also suprastructure. Total maxillectomy was performed in 4 patients, partial maxillectomy in 8 patients with orbital exenteration in 3 patients. Of 12 patients operated on three patients died one year, two patients two years and one patient 6 years after surgery. One patient is alive 12 years, three patients are alive three years and one patient is alive one year after surgery.

Adult↗
PubMed2001

Nucleolar organizer regions in laryngeal cancer.

The purpose of this study was to estimate the prognostic value of the AgNORs count per nucleus in laryngeal cancer. The sections of diagnostic, formalin-fixed and paraffin-embedded specimens from 48 patients with T1-4 tumours were stained with silver nitrate for visualization of NORs (AgNORs). The correlation between AgNORs score and tumour clinicopathological features was estimated. The mean AgNOR number per nucleus was 2.73 +/- 0.87 (range 1.48-4.38). The AgNOR counts did not correlate with the stage of the disease, patient survival rate and N stage. A significant correlation between the mean AgNORs number and various stages of histological differentiation of carcinoma was observed (p < 0.05).

Adult↗
PubMed2000

[The use of microsurgery and balneotherapy in the treatment of hyperplastic laryngitis].

The study aimed at the assessment of the clinical value of using the combined method of laryngeal microsurgery and balneotherapy in the operative treatment and prevention of recurrences of limited hyperplastic changes of the laryngeal mucosa. The study material comprised two groups of patients diagnosed and treated in the years 1991-1997 in the Lublin E.N.T. Department and Laryngological Research-Consulting Center in Iwonicz Zdrój. The I clinical group consisted of 194 patients in whom, on definite indications, micro-surgeries with Kleinsasser's method were performed and the II control group consisting of 165 subjects suffering from chronic simple inflammation of pharyngo-laryngeal mucosa without morphologic hypertrophy. Inhalant treatment was performed by means of an individual method--using inhalant devices by Thomex L-2 producing high density mist of 1-4 mu degree comminution. Isotonic Iwonicz saline-Elin 7 was used. Uniform criteria of assessment were assumed and used of direct and distant therapeutic effect (in control studies) for the whole group of patients in the study period. Obtained results were analysed statistically.

Adult↗
PubMed2000

[Pharyngeal closure after pharyngolaryngectomy].

Pharyngolaryngectomy was performed in 53 patients. In 36 patients pharynx defect was less than 50% of pharynx circumference and it was closed without reconstruction. Larger pharynx defects were closed using platysma myocutaneous flap (13 cases), pectoralis mayor flap (3 cases) and free forearm flap with microvascular anastomosis (1 case). Healing results are presented in each group of patients.

Adult↗
PubMed2000

[Expression of Bcl-2 antigen in laryngeal carcinoma].

The results of immunohistochemical investigations of BCL-2 presence in 50 patients with laryngeal carcinoma were presented. In 14% of all investigated cases presence of BCL-2 protein was observed. In patients with advanced clinical stage of laryngeal carcinoma higher expression of BCL-2 protein comparing with patients with lower clinical stages was more frequently observed--but it wasn't significant. No significant difference in presence of BCL-2 protein among the patients with a different stage of histological differentiation of carcinoma and among the patients with present and absent metastatic changes in regional lymph nodes was observed.

Adult↗
PubMed2000

[Expression of p53 antigen in laryngeal carcinoma].

The results of immunohistochemical investigations of p53 presence in 50 patients with laryngeal carcinoma were presented. In the whole investigated group the presence of p53 protein in 90% of all investigated cases was observed. In patients with advanced clinical stage of laryngeal carcinoma higher expression of p53 protein comparing with patients with lower clinical stages was more frequently observed--but it wasn't significant. No significant difference in presence of p53 protein among the patients with a different stage of histological differentiation of carcinoma and among the patients with present and absent metastatic changes in regional lymph nodes was observed.

Adult↗
PubMed1999

Expression of bcl-2 protein in lymphocytes of patients with laryngeal carcinoma.

The aim of this study was to evaluate the expression of bcl-2 protein in lymphocytes of the peripheral blood of patients with laryngeal carcinoma. The protein product of the proto-oncogene bcl-2 is a physiological inhibitor of apoptosis or programmed cell death. Since we believe that apoptosis is involved in the regulation of an immune response to a cancer process, we tried to show how this mechanism works in laryngeal carcinoma in comparison with normal peripheral blood lymphocytes of healthy controls. To explain the significance of this molecule's expression, we used flow cytometry to examine the expression of bcl-2 in T lymphocytes from the peripheral blood of 23 patients with laryngeal carcinoma and 20 healthy controls. Our study revealed that the expression of bcl-2 protein in T lymphocytes from the cancer patients was significantly higher than in the controls (P < 0.05). This difference in the expression of bcl-2 protein was found in both CD4 and CD8 subpopulations and was significantly higher than in the control group. In patients with laryngeal carcinoma expression of bcl-2 protein in T lymphocytes was higher in CD4 than in CD8 cells (P < 0.05). These results suggest that bcl-2 protein may interact in the regulation of apoptosis of lymphocytes, taking part in anti-cancer defence.

Adult↗
PubMed1999

Tumor angiogenesis in patients with laryngeal cancer.

Tumor angiogenesis was evaluated in 60 patients with primary laryngeal cancer by quantitating the microvessel density with antibodies against factor VIII. The microvessel density was then correlated with T stage, N stage, histologic grade and patient survival. A direct correlation was found between increased tumor angiogenesis and T stage, histologic grade and a shorter survival rate.

Adult↗
PubMed1998

[Epidemiologic and clinical tests of patients suffering from laryngeal cancer in the Lublin macro-region in the years 1991-1995].

The study of registered morbidity of patients suffering from laryngeal cancer in the Lublin macro-region in the years 1991-95 was carried out. Morbidity rates in the population in consecutive years of investigated 5-year age groups were examined. In clinical investigations the conditions of clinical advancement of the disease in particular organs was determined and environmental factors and occupation were also revealed.

Adult↗