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Biomedical subjects

J Koziorowska

Publications and source records attributed to J Koziorowska.

At least 37 records · Page 2Linked to original sources
PubMed1980

Changes in the properties of human fibroblasts in culture after treatment with vitamin A palmitate.

The behaviour of subcultures of vitamin A-treated human embryo fibroblasts was studied from the point of view of morphology, growth characteristics, karyology and agglutinability at subsequent passages after treatment. Evidence was obtained that vitamin A-induced rupture of lysosomes may lead to transient alterations in the pattern of growth, structure of the membranes and morphology in cell generations deriving from the cells injured by the action of the vitamin.

Acid Phosphatase↗
PubMed1980

Dependence on exogenous methionine of rat sarcoma and murine leukemia cells in culture.

A comparative study was performed on methionine auxotrophy of rat sarcoma and murine leukemia cells taken directly from the organism and grown in culture in media lacking methionine or in which methionine was substituted by homocysteine. Methionine auxotrophy was observed in both kinds of cells. At low levels of methionine in the media containing homocysteine rat sarcoma cells showed an increase in growth. Addition of homocysteine to the media with low levels of methionine did not influence the survival of murine leukemia cells.

Animals↗
PubMed1977

On the combined action of drugs on the lambda prophage induction in Escherichia coli K12 cells.

The capability of methotrexate, jododeoxyuridine and 5-fluorouracil to induce lambda prophage was compared when given alone or in combination. All these drugs were found to cause inducing conditions in Escherichia coli K12(lambda) cells. Combined action of jododeoxyuridine and methotrexate resulted in a pronounced increase in the number of free phages compared with that resulting the treatment either with methotrexate of jododeoxyuridine alone. Treatment with 5-fluorouracil caused inactivation of plaque forming ability in cells induced with methotrexate.

Coliphages↗
PubMed1975

Role of amino acid depletion in combined treatment of neoplastic cells with methotrexate and L-asparaginase.

The effect of pretreatment with L-asparaginase on the cytotoxicity of methotrexate (MTX) was studied in L5178Y murine leukemic cells and L-929 cells grown in vitro. The results were correlated with the effects of L-asparaginase on DNA synthesis. At low concentrations of the enzyme, pretreatment of cells enhanced the effect of MTX; at higher concentrations, it prevented the effect of MTX. Elimination of the MTX action was demonstrated also in L5178Y cells growing in the medium lacking L-asparagine. L-Asparagine readdition resulted in enhancement of MTX cytotoxicity. Pulse-labeling experiments demonstrated that in every case elimination of MTX action was related to a diminution of the fraction of cells in the phase of DNA synthesis. In contrast, enhancement of the effect of MTX was related to an increase in the fraction of cells in the DNA synthesis phase. A similar parallelism of effects occurred in L-929 cells when pretreatment with L-asparaginase was followed by washing the cells and culturing them in medium without the enzyme.

Animals↗