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J Lamvik

Publications and source records attributed to J Lamvik.

46 records · Page 3Linked to original sources
PubMed1982

Dissociation between tumour cell cytostatic and immuno-suppressive capacity of human monocytes activated with lymphokines.

Activation of monocytes with lymphokines from BCG-stimulated lymphocytes increased the capacity to inhibit DNA-synthesis in a tumour cell line (NHIK 3025) at all stages of monocyte maturation in vitro. However, the capacity of monocytes to suppress DNA-synthesis in mixed lymphocyte cultures (MLC) was reduced, indicating a dissociation of the immuno-suppressive and tumour cell cytostatic functions of lymphokine-activated monocytes. Supernatants of lymphokine-activated monocytes inhibited DNA synthesis in the tumour cell line, but not in MLC. Monocytes from donors with a strong tuberculin reaction produced supernatants with a stronger cytostatic activity than monocytes from donors with a weak tuberculin reaction. By in vitro maturation, even the later monocytes acquired ability to produce supernatants that were strongly inhibitory to tumour cells.

Cell Division↗
PubMed1979

Cytostatic effect on tumour cells induced in human monocytes by mediators from BCG-stimulated lymphocytes and MLC.

Human monocytes activated by mediators (lymphokines) from BCG-stimulated, sensitized lymphocytes (from BCG-vaccinated donors) were cytostatic to a human cell line. Mediators from allogeneic lymphocytes activated the cytostatic ability of monocytes to the same degree as mediators from autologous lymphocytes. Mediators from BCG-stimulated lymphocytes from tuberculin-negative donors not vaccinated with BCG, activated the monocytes only to a small extent. Culture of lymphocytes in a membrane chamber (MC) proximate to monocytes, or incubation of monocytes with filtered supernatants of lymphocyte cultures, were equally effective procedures for inducing cytostatic ability in monocytes. Supernatants of sensitized lymphocytes cultured on BCG for four hours did not activate the monocytes, while supernatants collected after 24 hours activated the cytostatic ability to the same extent as 72-hour supernatants. Supernatants of mixed lymphocyte cultures (MLC) collected after 24 hours did not activate the monocyte cytostatic ability at all. Forty-eight and 72-hour supernatants of MLC showed a small but increasing activity. There was no significant difference between BCG-stimulated lymphocytes and MLC in their maximum DNA synthesis or in the kinetics of their DNA synthesis. Thus, the DNA synthesis and secretion of lymphocyte mediators may be independent phenomena resulting from the same stimulus.

BCG Vaccine↗
PubMed1979

Activation of human monocytes by mediators from lymphocytes stimulated with Corynebacterium parvum.

Human monocytes activated in vitro by lymphokine-containing supernatants of autologous or allogeneic lymphocytes stimulated in vitro by Corynebacterium parvum (CP) expressed increased ability to suppress DNA-synthesis in a human tumour cell line. Monocyte activation was not dependent on in vitro differentiation of monocytes, enhanced cytostatic ability being observed at all stages of in vitro differentiation. The lymphokine-induced cytostatic ability was not affected by intensive washing and trypsin treatment of the activated monocytes, but disappeared during 48 hours of in vitro culture of the activated cells. The increased cytostatic ability of lymphokine-activated monocytes did not seem to be due to stable supernatant factors released from monocytes. CP stimulated DNA-synthesis in peripheral blood lymphocytes of 28 normal donors, thus confirming the mitogenic effect of CP on human lymphocytes. Lymphokine production in response to CP correlated with the magnitude of DNA-synthesis, but appeared before DNA-synthesis could be detected in the lymphocytes.

Cell Line↗
PubMed1977

Inhibitory effect of human mononuclear phagocytes on DNA synthesis in stimulated lymphocytes.

Human mononuclear phagocytes cultured in vitro revealed an inhibitory influence on DNA synthesis, as measured by 3H thymidine incorporation, in lymphocytes stimulated by a lectin (PHA), a soluble antigen (PPD) and allogenic lymphocytes. The inhibitory effect increased with increasing ratio of macrophages to lymphocytes, and was positively related to the differentiation of monocytes to macrophages. The inhibitory ability appeared to have no connection with the histocompatibility gene complex. The culture medium of macrophages cultured with and without lymphocytes showed no inhibitory effect.

Culture Media↗
PubMed1976

The effect of nitrogen mustard on human mononuclear blood cells.

The effect of nitrogen mustard on the function of human blood monocytes and lymphocytes cultured in vitro, was studied. After a single in vitro exposure to the drug at culture start, an inhibition of the survival of non-proliferating mononuclear phagocytes was observed. No immediate cytotoxic effect was registered. Nitrogen mustard given in therapeutic doses to patients with malignant lymphogranulomatosis did not reduce the survival of mononuclear phagocytes cultured at different times after the administration of the drug.

Adult↗
PubMed1976

The effect of phenylbutazone and chloramphenicol on phagocytosis of radiolabelled Candida albicans by human monocytes cultured in vitro.

The effect of phenylbutazone and chloramphenicol on the function of blood monocytes cultured in vitro was studied. Both drugs had an inhibitory effect on the engulfment stage of phagocytosis. While a moderate effect only of chloramphenicol on digestion of engulfed yeast particles was found, phenylbutazone caused a marked reduction of the digestion of engulfed particles. Concomitant with this reduction of digestive ability, the lysosomes showed no morphological alterations as observed in control cultures without drug addition, indicating a lack of fusion between the engulged particles and lysosomes in the presence of phenylbutazone.

Candida albicans↗
PubMed1975

[Medical education at the university of Trondheim].

In January 1975, medical education was started at the University of Trondheim, the fourth medical school in Norway. The educational planning committee for medicine has presented a complete curriculum comprising a two-year pre-clinical and a four-year clinical course. Working in co-operation with the Norwegian Institute for Hospital Research and the school of Social Work and Public Administration as well as with the faculties of social science, biology, and technology at the University of Trondheim, the medical faculty will have unique possibilities of development. This holds true for, e.g., occupational medicine, industrial hygiene, health administration, environmental protection, toxicology, biophysics, and medical technology.

Curriculum↗