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Biomedical subjects

J Lisiewicz

Publications and source records attributed to J Lisiewicz.

At least 19 recordsLinked to original sources
PubMed1992

[Qualitative determination of chemical compounds in the tissue of laryngeal cancer by x-ray microanalysis].

The authors presented the results of chemical compounds in the laryngeal cancer tissue in 20 patients with clinically and histologically confirmed plano-epithelial carcinoma using own modification of x-ray microanalysis method. Comparison between the healthy and neoplasmatic tissue showed as follows: 1) both healthy and neoplasmatic tissue showed the presence of Cd, Zn, Fe, Mn, Mg and Si, P, S, Cl, K, Ca, Ti; 2) the neoplasmatic tissue showed the presence of Pb whereas it was not observed in the healthy tissue; 3) both Pb and Cl were irregularly concentrated in the neoplasmatic tissue while in the healthy tissue showed regular concentration. The authors discuss some methodological aspects and try to interpret the obtained data in the view of toxic, mutagenous and carcinogenous role of the Pb.

Adult↗
PubMed1991

Effect of heparin and prostacyclin-heparin infusion on blood coagulation in haemodialysed patients.

In 10 patients (8 men and 2 women) aged 28 to 58 years (mean 44.4 years) treated by repeated haemodialysis due to end-stage renal failure, the bleeding time, whole-blood coagulation time, one-stage prothrombin time, thrombin time of plasma, activated partial thromboplastin time (APTT), fibrinogen level and euglobulin lysis time have been determined (1) during a 4-hour haemodialysis using heparin as an antithrombotic agent, and (2) one week later in the course of another haemodialysis using prostacyclin-heparin. The values for any of the above parameters with both anticoagulant treatment types did not differ. Plasma fibrinogen level after haemodialysis was significantly lower after administration of heparin alone as compared with the group treated by prostacyclin-heparin infusion. During haemodialysis performed with prostacyclin-heparin infusion, activation of the blood fibrinolytic system was manifested by a significant shortening of euglobulin lysis time, observed after 1.5 hours and after the end of haemodialysis. The above phenomenon did not occur when haemodialysis was performed with heparin alone.

Adult↗
PubMed1991

The trial of quantitative determination of the lead, chlorine, and sulfur content in the peripheral blood lymphocytes from patients with cancer of the larynx using the X-ray microanalysis method.

Using own modification of X-ray microanalysis method the authors evaluated the lead, chlorine, and sulfur content in the peripheral blood lymphocytes in 20 patients with laryngeal carcinoma in comparison with 20 healthy subjects of the control group. In patients with advanced clinical stages of that carcinoma the elevated lead and chlorine content has been stated in above cells whereas that of sulfur was decreased. According to the authors' opinion above changes reflect functional disturbances of lymphocytes in the patient with laryngeal carcinoma related to the decreased detoxicative properties, the intracellular electrolyte imbalance, and the enzymatic machinery alterations within that cells.

Adult↗
PubMed1991

[Activity of selected neutrophil enzymes of patients maintained on hemodialysis and treated with erythropoietin (rHu EPO)].

The peroxidase, alkaline phosphatase, acid phosphatase, beta-glucuronidase and N-acetyl-beta-D-glucosaminidase activity was assessed using a semiquantitative cytochemical methods in peripheral blood neutrophils from 10 maintenance haemodialysed patients treated with recombinant human erythropoietin (rHu EPO) due to severe anaemia. The examination was performed immediately prior to rHu EPO treatment, after 10 weeks and 32 weeks of therapy. A statistically significant increase in the beta-glucuronidase and N-acetyl-beta-D-glucosaminidase activity was observed after 10 weeks, while all the enzymes studied except peroxidase showed a significant elevation of their activity after 32 weeks of the treatment as compared with the values obtained prior to therapy.

Adult↗
PubMed1990

Beta-glucuronidase activity in neutrophils of patients with malignancies.

The human neutrophils induce cytotoxic effects on mammalian tumor cells. Hence it may be expected that intracellular enzymatic deficiencies of neutrophils may represent another cancer risk factor. In 205 patients with various malignancies the neutrophil beta-glucuronidase activity has been determined using a semiquantitative cytochemical method. A statistically significant deficiency of this enzyme in neutrophils has been observed in patients with precancerous states of the larynx, cancer of the larynx after radiotherapy and patients with cancer of large intestine. Patients with cancer of the lung and cancer of the stomach showed no changes with that regard whereas those with cancer of the breast demonstrated an increased enzyme activity.

Female↗
PubMed1979

The intracellular enzymatic response of neutrophils and lymphocytes in patients with precancerous states and cancer of the larynx.

In patients with precancerous states and cancer of the larynx prior to and after radiotherapy exhibit the decreased activity of neutrophil beta-glucuronidase. Moreover patients treated by radiotherapy before the age of 6 to 9 years demonstrate deficiency of N-acetyl-beta-glucosaminidase in the above cells. The main finding in lymphocytes of the patients studied was in the appearance by diffusion of the above enzymes and of acid phosphatase in the cytoplasm, reflecting their release from lysosomes and immunological mobilization of these cells. The authors discuss the possible role of neutrophil enzymatic deficiency in lowering the antitumour cytotoxic effect of these cells.

Acetylglucosaminidase↗
PubMed1979

Lymphocytes, neutrophils and serum immunoglobulins in patients with precancerous states of the larynx.

In 24 men aged 32 to 58 years with precancerous states of the larynx, i.e., leukoplakia, papillomas and pachydermia the peripheral blood lymphocytes were cytochemically stained for N-acetyl-beta-glucosaminidase, beta-glucuronidase, acid phosphatase and glycogen; and the neutrophils were stained for alkaline phosphatase, myeloperoxidase and lipids. The results were expressed in terms of the absolute counts of reaction-positive cells and of the activity index score. The serum immunoglobulins IgG, IgA and IgM were also determined by Mancini's method. The results obtained were compared with those in 20 healthy men aged 20 to 30 years. The patients exhibited elevated numbers of N-acetyl-beta-glucosaminidase and beta-glucuronidase-positive lymphocytes. A characteristic feature was an increase in the absolute counts of lymphocytes with diffuse and granular-diffuse types of cytochemical reaction for all enzymes studied. The number of cells with the granular type of enzymatic reaction (intact enzyme-positive lysosomes) was significantly diminished. These cytochemical alterations were accompanied by a significant increase in the serum IgA level. These results are discussed with reference to the lymphoid system response to tissues of precancerous lesions of the larynx. So far as the neutrophils are concerned the patients exhibited significant intracellular deficiency of beta-glucuronidase and decrease in the lipid content as well as an elevated alkaline phosphatase activity. The possible significance of the beta-glucuronidase deficiency in neutrophils for the diminished cytotoxic response of these cells against the tumor and precancerous lesion cells is discussed.

Adult↗
PubMed1979

[Thomson's colchicine test in differential diagnosis of lymphocytic leukemia and non-Hodgkin lymphoma].

The studies were performed to estimate the sensitivity of chronic lymphocytic leukaemia (CLL) lymphocytes (32 patients), non-Hodgkin lymphoma (NCL) lymphocytes (3 patients) and normal human (NH) lymphocytes (20 persons) to antimitotic concentration of colchicine in the conditions of the in vitro Thomson's test. Significant differences were observed in this sensitivity test: for CLL lymphocytes the mean values was 79%, for N-HL-lymphocytes 37%, for NH-lymphocytes--12%. These data show the possibility of practical use of Thomson's test in differential diagnosis of increased lymphocytosis. A quantitative heterogeneity of colchicine sensitive (pathological) and non-sensitive ("normal") CLL lymphocyte populations was observed among different CLL patients. There was no correlation between the proportion of the above populations and the patient's age, duration of illness and the magnitude of total lymphocytosis.

Adult↗
PubMed1979

Effect of radiotherapy on the neutrophil and the lymphocyte enzymatic equipment and serum immunoglobulins in patients with cancer of the larynx.

In 30 patients with cancer of the larynx, aged 40 to 70 years, treated by radiotherapy 6 to 9 years ago the decreased activity of neutrophil beta-glucuronidase and N-acetyl-beta-glucosaminidase accompanied by a decrease of absolute count of enzyme-positive cells was noted. Numbers of acid phosphatase-positive neutrophils were also decreased. Moderate increase of the neutrophil alkaline phosphatase activity and of numbers of enzyme-positive cells was another observed feature. The main finding in lymphocytes of the patients consisted in the appearance of cells exhibiting diffusion of lysosomal enzymes, especially of beta-glucuronidase, N-acetyl-beta-glucosaminidase and to a lesser degree of acid phosphatase into the cell cytoplasm. Moderate increase of immunoglobulin level, especially that of IgA, reflected probably the immunologic mobilization of patients.

Acid Phosphatase↗