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Biomedical subjects

J Marsala

Publications and source records attributed to J Marsala.

At least 145 records · Page 8Linked to original sources
PubMed1981

Incorporation of 14C-leucine into the proteins of spinal ganglia in vivo under conditions of partial ischaemia.

The authors studied the incorporation of 14C-leucine into the spinal ganglion proteins of dogs with a ligated abdominal aorta. The radioactivity of the protein fractions was studied after their electrophoretic separation in polyacrylamide gels in the presence of SDS. After 2-4 hours' ischaemia, incorporation of 14C-leucine into proteins with a molecular weight of about 100,000 fell compared with the control, while the radioactivity of proteins with a molecular weight of about 50,000 rose. The relative proportion of the protein fractions in the spinal ganglia did not alter significantly. Apart from these changes, certain qualitative changes occurred in the incidence of proteins with a molecular weight of about 40,000 during ischaemia.

Animals↗
PubMed1981

The division of the third ventricle in the segments of white rat.

In this paper the third brain ventricle (ventriculus tertius) of rat was studied on the frontal histological slices. Based on the change in the ventricle form and on the peculiarities of the surrounding nerve structures the third ventricle was divided into the upper and lower part and every part was divided into the segments denoted by capital T (tertius) and by the order number; the segments of the lower parts of the third ventricle were indicated as T 1 to T 4 and the segments of the upper part as T 5 to T 9. The adjacent part of aqueductus cerebri (A) was divided into the segments A 1 and A 2. The suggested method of the division aims to serve as an aid for the determination of the precise localization and the denomination of the examined parts of the third ventricle during the morphological studies.

Animals↗
PubMed1981

The division of the lateral ventricle into the segments in white rat.

The lateral brain ventricle (ventriculus lateralis) of the rat on the frontal serial histological slices was studied. Based on the change in the ventricle form in slices it was divided into the segments, marked by capital L (lateralis) and by the order number as segments L1 to L7. The method aims to serve as an aid for the simple denotation and easy identification of the examined part of the lateral ventricle during the morphological examinations.

Animals↗
PubMed1981

Distribution of free amino acids in the dog spinal cord, spinal ganglia and ischiadic nerve.

Levels of free amino acids were analysed in the cervical, thoracic, lumbar and sacral spinal cord of dogs. The highest content was in the sacral segment (20.2 mmol/kg wet tissue), next in the cervical (17.0) and in the lumbal segment (16.2). It was lowest in the thoracic segment (15.4). There was no difference in the content of glutamine and alanine in grey matter in comparison to white matter of posterior funicles. In the ventral horns the content of glycine and aspartic acid was markedly higher than in dorsal horns, on the other hand, the dorsal grey matter had a higher concentration of GABA than the ventral part.

Amino Acids↗
PubMed1980

Influence of ischemia on retrograde axonal transport in the posterior column fibers of the dog.

The retrograde axonal transport rate in the posterior spinal root was studied by means of the horseradish peroxidase injection in the control dogs and the dogs with the ischemia induced by the lumbal aorta ligature. As the results show, the increase in the retrograde axonal transport rate after 4 h ischemia occured from 141 +/- 6 mm/24 h to 270 +/- 31 mm/24 h. The retrograde transport rate after 7 d ischemia being 137 +/- 7 mm/24h reaches approximately the control values.

Animals↗
PubMed1980

Acetylcholinesterase activity and its molecular forms in selected regions of the dog's nervous system.

Acetylcholinestrase (AChE, EC 3.1.1.7) activity and its molecular forms were studied in the cerebral cortex, basal ganglia, thalamus, cerebellar cortex, medulla oblongata, the various parts of spinal cord, the spinal ganglia and the sciatic nerve in intact adult dogs. The number and the proportion of different molecular forms of AChE in these regions of the dog's nervous system varied considerably. The highest AChE activity was found in the basal ganglia, where only one molecular forms was detected. Activity was also high in the cerebellar cortex (2 isoenzymes), the thalamus (4 isoenzymes) and the medulla oblongata (5 isoenzymes), and was lower in the cerebral cortex (3 isoenzymes). AChE activity in the spinal cord was highest in the sacral part (4 isoenzymes); in the other parts (3 isoenzymes) it was equal. Five molecular forms were found in the spinal ganglia and two in the sciatic nerve. The significance of the multiple molecular forms of AChE is discussed.

Acetylcholinesterase↗
PubMed1980

Effect of ischemia on acetylcholinesterase activity and its molecular forms in the dog spinal cord, spinal ganglia and sciatic nerve.

Partial ischemia of the spinal cord was produced by the ligature of the abdominal aorta twice for 40 minutes with a 40 min. recirculation interval. At the end of the ischemic period acetylcholinesterase (AChE. EC 3.1.1.7) activity increased significantly in the lumbosacral part of the spinal cord but simultaneously decreased in the spinal ganglia; There was no significant change in the sciatic nerve. After 48 hours of recovery AChE activity returned to normal in the sacral part of the spinal cord and in the spinal ganglia, but a less expressive increase remained in the lumbal part. Electrophoretic separation of AChE on the polyacrylamide gel gave in intact control dogs: three or four molecular forms in the spinal cord, five in the spinal ganglia and two molecular forms in the sciatic nerve. Ischemia increased the relative content of the lower molecular forms and often a new low molecular form appeared.

Acetylcholinesterase↗