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J Marsala

Publications and source records attributed to J Marsala.

At least 127 records · Page 7Linked to original sources
PubMed1983

Cholinergic enzyme activity during partial brain ischemia in the dog.

Activity of cholinacetyltransferase (ChAT. EC 2.3.1.6) and acetylcholinesterase (AChE, EC 3.1.1.7) was monitored during occlusion of arteria cerebri media dx. (MCA) in five areas of the brain cortex, in nucleus caudatus and in the thalamus of the ipsilateral and contralateral hemisphere. After 1 hour of MCA occlusion ChAT and AChE activity was reduced in the ischemised region of the hemisphere, i. e. in gyrus ectosylvius anterior and gyrus sylvius anterior, whereas after 4 hours of occlusion the differences were not significant. In nc. caudatus and thalamus the activity of enzymes during ischemia did not change much.

Acetylcholinesterase↗
PubMed1983

Nucleus ultrastructure of dog dorsal root ganglia cells after partial ischemia.

The partial ischemia of the adult dogs, both males and females was induced by the ligature of aorta abdominalis above the branching of arteria coeliaca within 80 minutes and 120 minutes duration with the following preparation of L6 und L7 dorsal root ganglia. The nuclei and nucleoli of the dorsal root ganglia nerve cells were studied by the usual standard transmission electron microscopic procedures. Comparing the control and ischemized material we have observed the different reaction of the nuclei and nucleoli to the ischemia. Some of these structures remained unchanged, the others demonstrated a various degree of the alteration. After the partial ischemia some nucleoli were more compact, some nucleolonemata were less distinct but no karyoplasmic changes were noted. The nucleolar interstitial vacuoles were occasionally enlarged and the satellite bodies were observed scarcely in the proximity of the nucleolus.

Animals↗
PubMed1982

Free amino acids in the dog spinal cord during prolonged ischaemia.

The authors studied the effect of 40 and 80 minutes' occlusion of the abdominal aorta on the aspartic acid, glutamic acid, glutamine, glycine and gamma-aminobutyric acid concentration in the sacral, lumbar, thoracic and cervical segments of the dog spinal cord. The most important changes were displayed by the glutamine concentration, which rose significantly after ischaemia in all the regions studied. The alanine and GABA concentration rose in the sacral and lumbar segments respectively, while the glutamic and aspartic acid concentrations in the sacral region fell. At the same time, enzyme activities degrading the synthetic substrates Leu-p-nitroanilide and Gly-p-nitroanilide increased. The significance of the findings is discussed with reference to nerve cell catabolism and function.

Amino Acids↗
PubMed1982

Microtubular formations inside GER of dog spinal ganglion neurons.

Partial spinal cord ischaemia was induced in dogs, ranging from 1 to 3 years of age, by ligature of the abdominal aorta just below the arteria coeliaca. The changes of the lumbal sensitive ganglia neurons were studied. In addition to the most often occurring changes, e.g., the vacuolization of the mitochondria, the disaggregation of ribosomes, the dilatation of the Golgi complex, the microtubular formations in the dilated cisterns of the endoplasmic reticulum were observed. Modified cisterns of the granulated endoplasmic reticulum (GER) occurred focally in relatively well preserved neurons. The individual microtubules measured about 24 nm in outer diameter, and their walls were formed by some subunits.

Animals↗
PubMed1981

Effect of ischemia on the activity of DNA-dependent RNA polymerase and DNA polymerase.

Ischemia, anoxia, and hypoxia of the brain have been shown to inhibit protein synthesis in the central nervous system. To obtain data on the changes in DNA-dependent RNA and DNA polymerase as they pertain specifically to neurons and glia, nuclear enriched neuronal and glial fractions were prepared, by sucrose-gradient centrifugation, from spinal cords of adult dogs that had been subjected to prolonged ischemia. The isolated fractions were assayed for enzyme activity by a radiochemical technique. RNA polymerase was affected more than DNA polymerase, activity being reduced considerably in both neurons and glia. Possible causes of the difference in sensitivity to ischemia are discussed.

Animals↗
PubMed1981

Total content of RNA in individual nerve cells of spinal ganglion during ischemia in dogs.

The total RNA content in the individual nerve cells of the lumbal spinal ganglia in the dog after the 80 minutes lasting partial ischemia was followed. The spinal ganglion neurons did not react in ischemia in the same way. The total RNA content in the individual neurons of the control animals occurred in the range 132-990 arbitrary units, after the ischemia in the range 116-670 arbitrary units. In both groups the cells with the low or high RNA content were seen. The decreases in total RNA content after ischemia was at the higher level of the statistical significance in the group with the largest and middle large neurons giving the evidence about the greater sensitivity to the oxygen shortage in comparison with the group of the smallest spinal ganglion nerve cells.

Animals↗