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Biomedical subjects

J Mezei

Publications and source records attributed to J Mezei.

At least 19 recordsLinked to original sources
PubMed1986

Stability study of prostacyclin in solution.

The proton-catalyzed hydrolytic decomposition of prostacyclin was assayed by UV spectroscopic and HPLC methods. Changes of the absorbance in aqueous solutions were measured at 220 nm. HPLC studies were performed on C18 column using NH4Cl (0.03 mol X l-1)/NH4OH (0.03 mol X l-1)/methanol (1 : 1 : 4) eluent. Temperature and pH dependence of the hydrolytic process was determined. Effects of alcohol and glycols on the degradation rate were studied.

Chromatography, High Pressure Liquid↗
PubMed1984

A new method for high-performance liquid chromatographic determination of drotaverine in plasma.

A sensitive, specific high-performance liquid chromatographic procedure was developed for the determination of plasma drotaverine levels. Basic plasma samples were adjusted to pH 1.5 and extracted with chloroform. HPLC [n-heptane-dichloromethane-diethylamine (50:25:2)] on a microporous silica column, with a variable-wavelength UV detector set at 302 nm allowed the measurement of drotaverine at the 50-ng/mL level. The utility of this method for determination of drotaverine in dog and rat plasma was demonstrated.

Animals↗
PubMed1984

High performance liquid chromatographic determination of a new biologically active 1,2,4-oxadiazine derivative in rat plasma.

A sensitive high performance liquid chromatographic procedure was developed for the determination of 3-phenyl-6-(1,2,3,4-tetrahydro-2-isoquinolyl)methyl-4H-5,6-dihydro -1,2, 4-oxadiazine a new vasodilator, in plasma. Rat plasma samples were made alkaline with ammonia and partitioned with chloroform. The extracts were dried with anhydrous sodium sulphate and evaporated to dryness under reduced pressure. The residues were redissolved in chloroform, and then chromatographed on silica column with chloroform/isopropanol (7:3, v/v). Detection was performed with a variable-wavelength UV detector set at 265 nm. Limit of identification was 10 ng, that of quantitative determination 50 ng 1/ml of plasma. The utility of the method for pharmacokinetic studies in the rat was demonstrated.

Animals↗
PubMed1980

Investigation of the hydrolytic decomposition of 5-iodine-2'-desoxyuridine by high-performance liquid chromatography.

A new HPLC method was developed for the monitoring of the hydrolytic decomposition of 5-iodine-2'-desoxyuridine in aqueous solution and for the stability assay of this compound in formulation. The separation of 5-iodine-2'-desoxyuridine and its decomposition products (5-iodineuracil and uracil) was performed on a u Bondapack C18 column using a mixture of water and methanol as eluent. On the basis of the experimental data, the kinetic order and the rate-constants of both consecutive reactions were determined.

Chemical Phenomena↗