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Biomedical subjects

J Novák

Publications and source records attributed to J Novák.

At least 127 records · Page 7Linked to original sources
PubMed1986

Studies on satellite nucleoli in human normal and leukemic lymphocytes.

Lymphocytes from normal and leukemic patients, and phytohemagglutinin-stimulated lymphocytes were investigated by means of immunofluorescence procedures and a silver reaction for the demonstration of proteins characteristic of nucleolus organizer regions in interphasic cells to provide basic information on the presence of satellite nucleoli in these cells. The results clearly indicated that satellite nucleoli are present in a limited but constant percentage of peripheral lymphocytes. An increased percentage of lymphocytes with satellite nucleoli was found only in leukemic patients and after silver staining. In contrast, a decreased percentage of satellite nucleoli was found 24 h after stimulation with phytohemagglutinin vitro. In leukemic patients, the discrepancy in the percentage of lymphocytes with satellite nucleoli between immunostained and silver-stained preparations may suggest that the silver reaction demonstrates the presence of an additional argyrophilic protein besides proteins B23 and C23, or altered forms of these proteins, which does not react with the specific antibodies.

Cell Nucleolus↗
PubMed1986

[Serum level of antibiotics in burn patients].

Antibiotic serum levels were recorded from 100 patients with burns. Antibiotic doses to ensure effective serum levels were found to be in excess of maximum values quoted as necessary by manufacturers, provided that serum creatinine was normal. These findings were in agreement with literature data. This was the first instance to check on serum levels, following oral administration of antibiotics. Differences between individual patients may be considerable, probably due to variable renal blood supply and other factors. Individual dosage with control of serum levels is, therefore, recommended. Specific dosage recommendation for patients with burns would be desirable for hospital practice.

Adult↗
PubMed1985

The relationship between anaerobic performance and muscle metabolic capacity and fibre distribution.

Relationships between functional anaerobic indicators and the character of cellular muscle energy metabolism were studied. Twelve untrained male students were tested by a specific anaerobic test on the treadmill. The mean values of the anaerobic test were as follows: blood lactate 10.69 mmol . 1(-1), running speed 16.08 km . h-1 and duration 92.67 s. The average distribution of muscle fibres (m. vastus lateralis) was: type I 52.2%, type II A 29.0% and type II B 18.8%. The mean enzyme activity values were: triosephosphate dehydrogenase (TPDH) 4.67 mu kat . g-1 w.w., lactate dehydrogenase (LDH) 5.76 mu kat . g-1 w.w, citrate synthase (CS) 0.21 mu kat . g-1 w.w. and hydroxyacyl-CoA dehydrogenase (HAD) 0.12 mu kat . g-1 w.w. Significant negative correlations were found between delta LA and CS (r = 0.64) and % of fibre type II B and CS (r = 0.78) and positive correlations between % of fibre type I and CS and/or HAD (r = 0.60 and r = 0.62, respectively).

3-Hydroxyacyl CoA Dehydrogenases↗