Biomedical subjects
Publications and source records attributed to J Olszewski.
The authors showed the case of oesophageal perforation during an attempt to remove of foreign body at 37-year-old man. The foreign body (bone) was removed by right thoracotomy.
The evaluation of granulocytes function (PMN) in patients with chronic inflammation of middle ear and palatine tonsils. There were 39 people examined and divided into: 28 patients with chronic inflammation of middle ear and palatine tonsil, 11 healthy persons. The function of granulocytes in patients with chronic inflammation of middle ear and palatine tonsil were changed more in comparison with the healthy people.
The evaluation of oxygen species generation in blood granulocytes of patients with chronic inflammation of middle ear and palatine tonils. There were 38 patients tested (10 with chronic inflammation of palatine tonsil, 18 with chronic inflammation of middle ear and 10 healthy ones). There were statistically significant differences between the superoxide anion generation in patients with chronic inflammation of palatine tonsil and middle ear in comparison with to the healthy ones.
The evaluation of granulocytes function (PMNL) at patients with chronic hypertrophy of larynx and paranasal sinuses. There were 40 people examined and divided into: 10 patients with hypertrophy of larynx, 13 patients with hypertrophy of paranasal sinuses and 17 healthy people. There were evaluated: emission of chemiluminescence (CL), absorption of isotope (phagocytic index) in our modification migration in vivo and in vitro and the bacteriotoxic index. The function of granulocytes in patients with hypertrophy of larynx and paranasal sinuses were changed more in comparison with the healthy people.
The evaluated of oxygen specious generation in blood granulocytes of patients with hypertrophy of paranasal sinuses and larynx. There were 33 patients tested (13 with hypertrophy of paranasal sinuses, 10 with hypertrophy of larynx and 10 healthy). The superoxide anion generation of peripheral blood granulocytes described by the rate of the reduced cytochrome C at rest and after stimulation with opsonized zymosan according to Bellavite were measured. There were statistically significant differences between the superoxide anion generation in patients with hypertrophy of paranasal sinuses and larynx in comparison with the healthy ones.
The authors showed ophthalmical and orbital complications after operation of maxillary and ethmoid sinus in own material. Complication of optic organ (diplopia) was the most serious one.
Basing on the actual world literature authors present some complicated immunopathologic processes taking place in inner ear. Immunological mechanism loss of inner ear hasn't been taken into consideration by clinicists too frequent so far. At the same time diagnostic difficulties were pointed in this information in case of suspicion of inner ear disease basing on immunological process.
Basing on the actual world literature authors present opinions concerning methods of healing damages of internal ear caused by immunological processes. The following methods of healing internal ear damages are mentioned above: using of steroids in high doses for a long time, steroids and cytostatic drugs or cytostatic drugs itself (in case of bad toleration) or steroids parallelly with plasmaphoresis (as supporting method) or plasmaphoresis itself.
The authors described 26 cases with oesophageal foreign bodies during emergency service. The pork bone was the most frequent of oesophageal foreign body (57.7%).
The radioimmunoassayable concentration of insulin in isolated whey fractions of mammary secretion of sheep (n = 28) was measured. The concentrations were high on the last 2 days of pregnancy (550 +/- 80 and 580 +/- 77 microU/ml, respectively), higher on the day of parturition (780 +/- 71 microU/ml) and lower on the next day (290 +/- 54 microU/ml). During the following 4 consecutive days the concentrations gradually decreased to an almost basal blood level (14 microU/ml). The high insulin concentration in the mammary secretion during the periparturient period might be the result of intensified transfer of insulin through the blood-mammary barrier and increasing needs for this hormone by developing mammocytes and probably for the newborn lambs (n = 42) adapting to extra-uterine life. Conversely to the consecutive decrease in insulin, progressively increasing concentrations of sugars in mammary secretions were observed (from 2.1 +/- 0.22% on the second day prior to parturition to 5.5 +/- 0.13% on the fifth day post partum.
A case is presented of a patient with multiorgan trauma. The main diagnostic problem was coexisting haemorrhage. Curable skin defects were healed after several covering them with intermediate thickness mesh grafts. The postoperative course was complicated with sepsis and acute non-inflammatory renal failure with polyuria.
Using the neck rotation test during examination of Doppler's blood flows in vertebral arteries and basilar artery, the authors examined 60 subjects aged 16-60 years, including 20 healthy ones and 40 with cervical spondylarthrosis. The Transpect-TCD was used. Half the patients with cervical spine changes had also receptive hearing damage of low degree. The study demonstrated that neck rotation affected of patological Doppler's blood flows in one or in both vertebral arteries at 50% patients with cervical spondylarthrosis, but without clinical symptoms, and at 70% patients with cervical spondylarthrosis and clinical symptoms. The neck rotation test can have diagnostic significance by examination of sufficiency in vertebrobasilar system, or qualitative significance to microsurgical treatment.
The authors show case of lateral cervical cyst and carcinoma of the tonsil in woman 50 years, paying attention to large diagnostic and therapeutic difficulties. After operation of the lateral cervical cyst on the left side, metastasis of tumor followed, which was operated (after negative result of the thin-needle biopsy method) and histopathological examination of the tumor didn't prove atypical cells. In a few month after operation there followed tumorous infiltration in region angle of mandibula and in tonsillar region on the side and performed the thin-needle biopsy method showed planoepithelial carcinoma of the tonsil with transitional cells, which infiltrated the lingua, fundus of the oris and lymphonodes of the neck. Next the patient was sent to further treatment in the Oncological Hospital in Lodz. In connexion with advanced of the carcinoma, the using treatment (radiotherapy Co--60 and chemotherapy) was only the palliative treatment and prolongated life of the patient about 3 years.
RNase III is an endonuclease involved in processing both rRNA and certain mRNAs. To help determine whether RNase III (rnc) is required for general mRNA turnover in Escherichia coli, we have created a deletion-insertion mutation (delta rnc-38) in the structural gene. In addition, a series of multiple mutant strains containing deficiencies in RNase II (rnb-500), polynucleotide phosphorylase (pnp-7 or pnp-200), RNase E (rne-1 or rne-3071), and RNase III (delta rnc-38) were constructed. The delta rnc-38 single mutant was viable and led to the accumulation of 30S rRNA precursors, as has been previously observed with the rnc-105 allele (P. Gegenheimer, N. Watson, and D. Apirion, J. Biol. Chem. 252:3064-3073, 1977). In the multiple mutant strains, the presence of the delta rnc-38 allele resulted in the more rapid decay of pulse-labeled RNA but did not suppress conditional lethality, suggesting that the lethality associated with altered mRNA turnover may be due to the stabilization of specific mRNAs. In addition, these results indicate that RNase III is probably not required for general mRNA decay. Of particular interest was the observation that the delta rnc-38 rne-1 double mutant did not accumulate 30S rRNA precursors at 30 degrees C, while the delta rnc-38 rne-3071 double mutant did. Possible explanations of these results are discussed.
The paper presents some aspects of radiation hazard which occurs in a non-nuclear sector of industry, namely radiation hazard in non-uranium underground mines. The radiation hazard is caused in each type of underground mine by the naturally occurring noble radioactive gas-radon (222Rn) and radioactive products of its decay 218Po, 214Pb, 214Bi/214Po the so-called 'radon daughters' occurring in the mines' air. The paper presents the concept of how to provide a reliable system of assessment of miners' exposure by application of representative individual dosimetry, and also presents principles of computer-aided methods for interpretation of the results of miner's dosimetry useful for conversion of dosimetry data to the term of expected risk of cancer caused by exposure at miner's workplaces. The representative Individual Dosimetry system strengthened by computer-aided methods of analysis of results provided essential information on radiation cancer risk for miners employed in coal mines, metal-ore mines, chemical raw material mines in Poland. The coefficient of annual cancer risk induction is 1.5 x 10(-4) year-1 for coal mines, 1.40 x 10(-4) year-1 for metal ore mines and 1.5 x 10(-4) year-1 for chemical raw material mines. The radiation risk appears to be of the same magnitude as the conventional risk of life loss at work-related accidents. The average Lost Life Expectancy coefficient for both the radiation risk and conventional risk are 0.5 and 0.3 year per each miner, respectively.
The oxidation of low density lipoprotein (LDL) may be important in atherosclerosis. LDL can be oxidized by cultured cells, including macrophages and endothelial cells. This cellular oxidation is dependent on transition metal ions in the medium. We now report that LDL oxidation by endothelial cells and macrophages is caused by cell-dependent appearance of thiol in the medium ("thiol production"). Thiol appeared in medium when cells were incubated under standard serum-free culture conditions. L-Cystine in the medium was required for thiol production and also for LDL oxidation. Cell-dependent appearance of thiol was inhibited by glutamate (which blocks cystine uptake) and by diethylmaleate (which reacts with thiols). Both compounds also blocked cellular LDL oxidation, even though neither compound had antioxidant activity. Finally, we designed an enzymatic system, based on glutathione reductase, that mimicked cellular thiol production. This enzymatic system caused LDL oxidation, and showed the same dependency for transition metal ions as did cellular LDL oxidation. We conclude that in media containing transition metal ions, cellular oxidation of LDL can be explained by the cell-dependent appearance of thiol in the medium. A very similar mechanism was proposed in 1987 by Heinecke et al. (J. Biol. Chem. 262: 10098-10103). Under other conditions, however, cellular oxidation of LDL may occur by other mechanisms.
The results of preliminary tests of natural ionising radiation performed in the area of mining plants and their surroundings have been presented. The survey included measurements of radon concentration and gamma-ray dose rate in free air at 25 locations, 40K, 226Ra and 228Th determination in 48 samples of ore, ore concentrate, floatation tailings, rock and soil as well as 226Ra determination in 11 samples of mine water and river. It was found that the natural radioactivity of ore, rock, concentrate and tailings from mine is higher than the environmental background. For that reason a technologically enhanced natural radiation may occur at some places of mining plants and surrounding areas. Those places must undergo full dosimetric investigation and be under control. It was impossible to estimate how much the environment is contaminated by radon and water coming from mine. However one can say that generally the gamma ray background and radon concentration in free air of this region are at the level considered as a normal.
The aim of this work was comparative evaluation of the cervical nystagmus in cervical torsion test with the Nylen's positional nystagmus and their diagnostic value in correlation from anatomical state of cervical column. The authors examined 40 patients with degeneration of cervical spinal column and with normal radiograph of cervical spinal column. The obtained results showed that the cervical nystagmus in cervical torsion test was more frequent (47.5%) than the Nylen's positional nystagmus (37.5%). The cervical nystagmus in cervical torsion test was observed also in health subjects. The authors think that the above mentioned deviations can be caused by the discreet degeneration of cervical spinal column, unseizable in radiograph. The examinations showed that the cervical nystagmus in cervical torsion test could be used simultaneously with the Nylen's positional nystagmus in diagnostic of vertigo, first at all in patients with degeneration of cervical spinal column.