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J Piekarska

Publications and source records attributed to J Piekarska.

At least 19 recordsLinked to original sources
PubMed2002

Modulatory effects of calf thymus extract on the subset of T lymphocytes in Trichinella spiralis-infected mice.

The immunotropic properties of calf thymus extract (TFX-Jelfa) is connected with the mimic action of the thymus to modulate the differentiation, maturation and function of prothymocytes and mature thymus dependent (T) cells. The studies were carried out on CFW male mice aged 3 months. The animals were infected per os with 200 larvae of Trichinella spiralis. TFX-Jelfa was administered i.p. at a dose of 10 mg/kg seven times at 24 hour intervals prior to infection. The percentage of CD4+ and CD8+ in suspension of splenocytes and mesenteric lymphonode cells by flow cytometry using monoclonal antibodies coupled with fluorescein isothiocyanate (FITC) or phycoerythrin (PE) were determined. At the same time, cryostat preparations, made from jejunum and muscle samples, were examined by the direct immunofluorescence method using FITC-labeled antibody to mouse CD4+ and CD8+. It has been found that infection with T. spiralis in mice decreases the percentage of CD8+ splenocytes, while the percentage of CD8+ mesenteric lymphonode cells does not change. However, in infected mice the percentage of CD4+ spleen cells and mesenteric lymphonode cells is increased. It has been also found that during the course of infection an increase in the number of CD8+ and CD4+ cells in the basal lamina propria of the intestines was observed. In infected mice, CD4+ lymphocytes were visible in the inflammatory infiltrates of the muscle tissue on the 14th day, whereas CD8+ lymphocytes were first observed a week later. Pretreatment with TFX does not change the inhibitory effect of infection on the percentage of CD8+ splenocytes, but potentiates the percentage of CD4+ spleen cells and mesenteric lymphonode cells increased by infection. Furthermore, administration of TFX prior to infection also potentiates the stimulatory effect of T. spiralis on the number of CD8+ and CD4+ in the basal lamina propria of the jejunum, and on the number of CD8+ cells in the inflammatory infiltrates of the muscle tissue.

Animals↗
PubMed2001

[Trials of penetration modification by inflammatory infiltrating cells into Trichinella spiralis larva capsules].

The inflammatory infiltration cells penetrating through the larval capsule walls were observed. The observation were carried out in the masseter muscle of the mice infected with Trichinella spiralis larvae only (control) or infected and treated with PHA-P, TFX or dexamethason. The more numerous cells, than in the control, in the mice treated with PHA-P and TFX were seen. Opposite, the fever numerous cells inside the capsula walls in the mice that received dexamethason were found. The authors supposed that the phenomenon of inflammatory cells penetration through larval capsule play an important role in the defence mechanisms of the host.

Animals↗
PubMed2001

Attempts of modification of apoptosis in the course of experimental trichinellosis in mice.

The influence of the some immunomodulators (PHA-P, TFX and dexamethasone) on the process of apoptosis, occurring in the course of trichinellosis in mice, has been studied. It has been found that PHA-P activates this process in the jejunum mucosa and prolongs it in the muscular inflammatory infiltration, whereas TFX has no influence and dexamethasone distinctly decreases the level of the apoptotic cells. The number of the intestinal trichinae on the successive days of infection was similar in all groups of animals, however, the number of the muscular larvae in the groups receiving immunostimulators was much lower and in the group treated with dexamethasone--a little higher than that in control. As in mice receiving PHA-P and TFX, the cellular inflammatory infiltration in the muscles was larger than that in control, and in the group to which dexamethasone was administrated--smaller, the authors think that it was extensiveness of the infiltration and not the level of the apoptotic cells that influenced the number of the outliving larvae.

Animals↗
PubMed2000

The phenomenon of apoptosis in the course of experimental trichinellosis in mice.

Mice infected with 200 Trichinella spiralis larvae were killed at 3-69 days post infection (dpi) and the jejunum and masseter muscles were sectioned in a cryostat and examined in the Tunell method with "In situ Cell Detected Kid POD" of Boehringer-Mannheim. Data concering the exact localization and dynamic of apoptic cells in both organs are presented. The authors conclude that apoptosis plays a important role in the pathogenesis of trichinellosis.

Animals↗
PubMed1989

Zinc, vitamins A and E, and retinol-binding protein in sera of patients with cancer of the larynx.

Serum vitamin A concentrations were measured in 22 patients with laryngeal cancer and found to be significantly lower than those of patients of similar age with either nonmalignant laryngeal or other than laryngeal diseases. The levels of vitamin A in the patients with laryngeal cancer, but not in the controls, significantly correlated with serum concentrations of retinol-binding protein (RBP) and zinc. It is suggested that low levels of zinc might reduce the synthesis of RBP and thus reduce the mobilization of vitamin A from the liver stores.

Aged↗
PubMed1987

Adenosine deaminase and purine phosphorylase activities in lymphocytes and red blood cells of patients with carcinoma of the larynx.

Lymphocytes from patients with cancer of the larynx have been characterized by the activity of two enzymes involved in purine metabolism--adenosine deaminase and purine nucleoside phosphorylase. It has been shown that purine nucleoside phosphorylase activity was 1.5-fold higher in lymphocytes of patients in comparison with a group of healthy individuals. Adenosine deaminase activity in lymphocytes was elevated three to four-fold. The authors suggest that especially the estimation of adenosine deaminase activity in lymphocytes would be a useful laboratory test in the study of immune responsiveness of patients with cancer of the larynx.

Adenosine Deaminase↗
PubMed1987

N-acetyltransferase phenotype of patients with cancer of the larynx.

In the present paper N-acetyltransferase phenotype has been evaluated in 128 patients with cancer of the larynx. Out of 128 patients, 86.7% were smokers. The corresponding control group consisted of 106 men who did not suffer from neoplastic diseases. 83.6% of the patients were slow acetylators. This is significantly different from the frequency of 60.3% of slow acetylators observed in the control group. The slow acetylators exhibited a greater susceptibility to the cancer of the larynx.

Acetylation↗