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J S Deng

Publications and source records attributed to J S Deng.

At least 55 records · Page 3Linked to original sources
PubMed1986

Discordance of SSA/Ro and SSB/La cellular antigens in synchronized cells.

SSA/Ro and SSB/La are soluble cellular proteins to which antibodies are frequently produced in patients with Sjögren's syndrome and systemic lupus erythematosus. In this investigation, we examined anti-SSA/Ro and anti-SSB/La staining patterns on synchronized WiL2 cells and mixed lymphocyte culture cells using monospecific antisera. In addition to its presence in the nucleoplasm, the SSB/La antigen was highly concentrated in the nucleolus of cells during the late G1 and early S phase and is thus cell cycle-related. In contrast, the SSA/Ro antigen was found to be independent of cell cycle, showing a nuclear speckled pattern in all phases. Blocking experiments indicated that free SSB/La is responsible for the nucleolar staining, whereas the combination of both SSA/Ro and SSB/La determines the nucleoplasmic speckled staining pattern.

Antibodies, Antinuclear↗
PubMed1985

The binding of antihistone antibodies to Crithidia luciliae kinetoplasts is growth cycle-dependent.

The Crithidia luciliae immunofluorescence (CLIF) assay is widely used to test for native DNA (nDNA) antibodies in the diagnosis and management of systemic lupus erythematosus. However, sera from patients with drug-induced lupus erythematosus or rheumatoid arthritis, which should not contain nDNA antibodies, occasionally react with the CL kinetoplast. We examined 36 sera from patients with systemic lupus erythematosus, rheumatoid arthritis, Sjögren's syndrome, and drug-induced lupus erythematosus, who had positive CLIF tests. All 36 sera were also antinuclear antibody-positive with homogeneous and/or peripheral staining patterns on mouse kidney substrates. After hydrochloric acid extraction of the CL smears to remove histone and other nuclear protein antigens, 14 of the 36 sera no longer produced a positive result on the CLIF test. Ten of these 14 sera again gave a positive CLIF result after the hydrochloric acid-extracted Crithidia substrate had been reconstituted with purified histone. These studies demonstrated that kinetoplast binding was due to antihistone antibodies in at least 10 of 36 initially CLIF-positive sera. Antihistone antibodies were then purified with a histone-affinity column, and these purified antibodies were reactive with CL kinetoplasts. Thus, the CLIF test is not specific for nDNA antibodies. Additional studies using CL from different days of culture indicated that histone antigen expression in the CL kinetoplast was a function of the life cycle of this organism and is most readily detected 2 days after initiation of culture.

Animals↗
PubMed1985

Effect of actinomycin D on the expression of nuclear antigen SS-B/La.

The nuclear autoantigen SS-B/La, which is recognized by antibodies in certain autoimmune diseases, is complexed with distinct cellular RNAs and the complex of antigen-RNA profile remains constant throughout the cell cycle. This antigen is prominent in the nucleolus during G1/S phase of the cell cycle. Actinomycin D at a concentration of 0.0005 microgram/ml is capable of abolishing the appearance of SS-B/La in the nucleolus. The amount of immune-precipitable SS-B/La-associated RNAs is reduced by 0.005 microgram/ml actinomycin D treatment. The mechanism for these findings is suggested to be related to inhibition of the synthesis of messenger RNA coding for SS-B/La.

Antigens↗
PubMed1985

Molecular characteristics of SS-B/La and SS-A/Ro cellular antigens.

Anti-SS-B/La and anti-SS-A/Ro antibodies coexist in certain patients with connective tissue diseases such as systemic lupus erythematosus or Sjögren's syndrome. The respective antigenic structures with which these autoantibodies bind have not been fully characterized. The present study was conducted to better define these two different cellular antigens. WiL2 cell extracts were used to obtain partially purified SS-B/La and SS-A/Ro antigens. Both were found to be present in most fractions obtained after sequential purification with ammonium sulfate salt precipitation, G-200 gel filtration, DE-52 ion exchange chromatography, and preparative slab gel electrophoresis. However, SS-B/La antigenic activity was also found to be present in some fractions that did not contain detectable SS-A/Ro activity. These findings suggested the existence of two different forms of SS-B/La antigen: one containing the SS-B/La antigen only and the other containing both the SS-B/La and SS-A/Ro antigens. The RNA and protein components of these two ribonuclear protein particles were further defined by immunoprecipitation experiments using 32P-labeled WiL2 cell extract. The SS-B/La antigen was found to be associated with several RNAs while the SS-A/Ro antigen was associated with several other distinct RNAs. Both antibodies precipitated a common 43K molecular weight phosphoprotein. The antigenic peptides of these 2 antibodies were analyzed using an immunoblot system. The SS-B/La antigen was present on a 43K peptide which was unstable and could be degraded to several peptides of lower molecular weight (40K, 38K, 30K), while the SS-A/Ro antigen occurred on a peptide having a molecular weight of about 60K.

Antibodies, Antinuclear↗
PubMed1985

Expression of Ro/SS-A antigen in human skin and heart.

Using human sera monospecific for anti-Ro/SS-A antibodies in an indirect immunofluorescence assay, Ro/SS-A antigen was found to be present in nuclei of human fetal ectodermal cells and newborn epidermal keratinocytes, but not in adult epidermal keratinocytes. After in vitro cultivation, Ro/SS-A antigen was also found in the nuclei of adult skin explant outgrowth cells. The localization of Ro/SS-A in fetal heart could not be precisely determined (possibly nuclear and membrane). Extracts from fetal skin and heart contained Ro/SS-A antigen which is identical to the Ro/SS-A present in WiL2 cell extract. The molecular weight of Ro/SS-A antigenic peptide present in these extracts is around 60K.

Adult↗
PubMed1984

The importance of tissue substrate in the SS-A/Ro antigen-antibody system.

Using highly monospecific anti-SS-A containing sera and specific antibody to SS-A antigen dissociated from immune precipitates, tissue sections and cell culture lines were evaluated by the indirect immunofluorescence technique to determine the intracellular location, tissue distribution, and species specificity of the SS-A/Ro antigen. The SS-A/Ro antigen is predominantly a nuclear antigen giving an immunofluorescence staining pattern of discrete nuclear speckles. The SS-A/Ro antigen is present in a wide variety of human tissue, including kidney and liver parenchymal cells, lymphocytes, fibroblasts, and epithelioid cells. However, the SS-A/Ro antigen does appear to have a variable species distribution, with significant quantities of the antigen detected by immunofluorescence in cells of human, monkey, dog, and guinea pig, but absent to low amounts detected in cells of mouse, rat, rabbit, hamster, and chicken.

Animals↗
PubMed1984

The role of RNP, Sm, and SS-A/Ro-specific antisera from patients with lupus erythematosus in inducing antibody-dependent cellular cytotoxicity (ADCC) of targets coated with nonhistone nuclear antigens.

To better understand potential antibody-dependent mechanisms of tissue damage in lupus erythematosus (LE), an examination of whether antibodies to nonhistone nuclear antigens in LE patients' sera can induce ADCC of cellular targets coated with the corresponding antigens was undertaken. With high titer anti-RNP sera, significant ADCC was seen with monocyte (P less than 0.01), T-lymphocyte (P less than 0.001), and low-density lymphocyte (P less than 0.001) effectors. Using monocyte effectors, significant ADCC was seen with anti-RNP (P less than 0.01), anti-Sm (P less than 0.01), and anti-SSA/Ro (P less than 0.01), with the most profound lysis being with the anti-SSA/Ro sera. Neutrophils were ineffective in any nuclear antigen-antibody system tested. The effective mononuclear cell-mediated ADCC seen with anti-RNP, anti-Sm, and anti-SSA antisera may be related to the mononuclear cell-associated tissue change seen in cutaneous lupus lesions.

Antibodies↗
PubMed1984

Reevaluation of specificity of Crithidia luciliae kinetoplast as a substrate for detecting antibodies to double-stranded deoxyribonucleic acid.

Until now, the kinetoplast of the hemoflagellate Crithidia luciliae has been believed to be a specific indirect immunofluorescence substrate for detecting antibodies to double-stranded deoxyribonucleic acid (DNA). We have recently identified a patient in whom there was the apparent presence of procainamide-induced antinuclear antibodies at a titer of 1/1,280 on mouse kidney sections. This patient's serum also contained antihistone antibodies at a titer of 1:1,280, on the basis of a mouse kidney section histone reconstitution assay. In addition, results of an enzyme-linked immunosorbent assay (ELISA) using a pure histone preparation were strongly positive. We found that this serum produced C. luciliae fluorescence to a titer of 1:80. After pretreatment of the C. luciliae substrate with hydrochloric acid to remove histones, this serum no longer produced kinetoplast fluorescence. However, this serum again produced kinetoplast fluorescence when assayed on a hydrochloric acid-treated C. luciliae substrate that had been reconstituted with purified histone. The negative kinetoplast reactivity after hydrochloric acid treatment of the C. luciliae substrate suggests that the antibody is reacting with acid-extractable nucleic acids, not necessarily the double-stranded DNA, or the antibody is directed against nuclear proteins such as DNA-histone complexes of deoxyribonucleoprotein. The finding that reactivity reappeared after the histone was reconstituted back to the hydrochloric acid-treated C. luciliae substrate suggests that the primary antibody in this serum is directed against DNA-histone complexes or histone alone. These findings strongly suggest that the kinetoplast of C. luciliae contains antigens other than double-stranded DNA and that is not a specific substrate for the detection of double-stranded DNA antibodies by indirect immunofluorescence.

Adult↗
PubMed1984

Relationships between antinuclear and anti-Ro/SS-A antibodies in subacute cutaneous lupus erythematosus.

A certain degree of confusion has arisen regarding the relationship between patients having subacute cutaneous lupus erythematosus (SCLE) and those with "antinuclear antibody-negative" systemic lupus erythematosus (ANA-negative SLE). One of the confusing issues relates to published differences in the autoimmune serologic findings of these two patient groups. In order to clarify this issue, we have screened the sera from thirty-seven patients with SCLE for the presence of fluorescent antinuclear antibodies (FANA) on two different substrates--human Hep-2 tissue culture cells and mouse kidney sections. In addition, these same sera were assayed for anti-Ro/SS-A precipitin antibodies. Seventy-eight percent of the sera were FANA-positive at a titer of 1:10 or greater when tested on human Hep-2 cells, whereas 76% were positive at a titer of 1:80 or greater. Fifty-one percent were positive on mouse kidney sections at a titer of 1:10 or greater, whereas 46% were positive at a titer of 1:20 or greater. Twenty-two percent of these sera were completely FANA-negative on both human and mouse substrates. None of these sera that were negative on both substrates contained anti-Ro/SS-A antibodies. However, 69% of the sera that were FANA-positive on both human and mouse substrates were found to have detectable anti-Ro/SS-A antibodies. Ninety percent of the SCLE sera that were FANA-positive on human Hep-2 cells, but negative on mouse kidney sections, contained anti-Ro/SS-A antibodies. These sera gave a speckle-like, or particulate, nuclear immunofluorescence staining pattern.(ABSTRACT TRUNCATED AT 250 WORDS)

Acute Disease↗
PubMed1984

Reappraisal of the specificity of the Crithidia luciliae assay for nDNA antibodies: evidence for histone antibody kinetoplast binding.

Five different high-titer histone antibody-containing sera were assayed by the Crithidia luciliae indirect immunofluorescence (CLIF) technic. Three of these sera produced kinetoplast binding at titers of 1/40 to 1/80. The kinetoplast binding activity was abolished by HCl acid pretreatment of the Crithidia substrate, suggesting that the kinetoplast binding activity was not due to antibodies against native DNA (nDNA). Histone antibodies were purified from two of the three positive sera by affinity chromatography utilizing purified preparations of histone. Both purified antibody preparations also had kinetoplast-binding activity, confirming that the Crithidia kinetoplast contains histone-like proteins. Therefore, Crithidia luciliae (CL) kinetoplast binding activity does not necessarily indicate the presence of anti-nDNA antibodies. Routinely pretreating the CL substrate with 0.1 N HCl would eliminate the possibility of histone antibody kinetoplast binding in the CLIF assay. Whether such pretreatment would alter the binding of anti-NDNA to the kinetoplast remains to be determined.

Antibodies↗
PubMed1984

Comparison of substrates for the detection of antinuclear antibodies in normals and in patients with connective tissue and other diseases.

There has been some controversy regarding the relative merits of cell lines versus frozen tissue substrates for the detection of antinuclear antibodies (ANA) by indirect immunofluorescence. We have compared two cell lines (KB and HEP2) with frozen mouse kidney for the detection of ANA in several groups of individuals. Cell lines were more likely to detect ANA than frozen mouse kidney in normal individuals and in hospital and clinic patients with diseases other than connective tissue diseases when sera were examined at manufacturer's recommended screening dilutions. There was also a trend for the cell lines to demonstrate ANA more frequently than mouse kidney in patients with systemic lupus erythematosus and other connective tissue diseases, but the differences were not statistically significant. Centromere antibodies could be reliably suspected only on cell lines and could be confirmed only if mitotic figures were present.

Adolescent↗
PubMed1983

Antinuclear and anticytoplasmic antibodies. Concepts and misconceptions.

Skin involvement is a common feature of several diseases in which circulating autoantibodies to nuclear and cytoplasmic antigens can be found. Patients with these diseases can be managed more efficiently if the physician is fully aware of the diagnostic and prognostic value of these various antinuclear and anticytoplasmic antibodies. The dermatologist, therefore, not infrequently, must face the bewildering task of ordering and interpreting the results of assays for an ever-increasing number of circulating autoantibodies. In this report, we have attempted to make this less burdensome for the clinician by pointing out the basis for some of the confusion that has arisen in this area. The confusion has resulted primarily from a failure to recognize the limitations of the various assays involved.

Antibodies, Antinuclear↗
PubMed1983

Characterization of a phosphoprotein associated with the SS-B/La nuclear antigen in adenovirus-infected and uninfected KB cells.

We have employed sera from patients with autoimmune disease to characterize the nuclear SS-B/La antigen in uninfected and adenovirus-infected KB cells. A 45,000-dalton phosphorylated polypeptide was specifically precipitated with anti-SS-B sera, and the level of phosphorylation was increased after infection. The increased phosphorylation appears to occur at the same amino acid residues phosphorylated in uninfected cells and results from increased phosphorylating activity rather than from altered enzyme specificity. A competition experiment between infected and uninfected cell extracts indicates that the antigen in the infected cell binds more strongly to SS-B/La antibodies. Fragments of adenovirus-induced virus-associated RNA as well as intact molecules complex with SS-B/La antigen and are immune precipitated with autoimmune sera.

Adenoviridae↗
PubMed1981

A nuclear antigen associated with cell proliferation and blast transformation.

A nuclear antigen associated with cell proliferation (proliferating cell nuclear antigen-PCNA) and blast transformation is recognized by autoantibodies in the sera of some patients with systemic lupus erythematosus. This autoantibody is a precipitating antibody and also reacts in immunofluorescence, staining the nucleoplasm of proliferating and blast-transformed cells. The autoantibody was used as a reagent to determine the distribution of PCNA in a synchronized continuous B lymphoid cell line (WiL-2) and in mitogen-induced blast-transformed lymphocytes. In WiL-2 cells, PCNA was detected as speckled nucleoplasmic staining in G1, S, and G2 phases of the cell cycle. In addition, during late G1 and early S phases, PCNA was also detected in the nucleolus. During mitogen-induced blast transformation of lymphocytes, PCNA was noticed in the nucleolus before the initiation of DNA synthesis and later became nucleoplasmic with disappearance of nucleolar staining. These studies demonstrate that the relationship of PCNA to proliferation and blast transformation may be associated with events related to DNA synthesis in these cells.

Animals↗
PubMed1981

Nonhistone nuclear antigens reactive with autoantibodies. Immunofluorescence studies on distribution in synchronized cells.

Sera from patients with certain autoimmune diseases tht contained autoantibodies to nonhistone nuclear antigens were used as reagents in an indirect immunofluorescent study. The distribution of these nuclear antigens was determined in synchronized human B lymphoid cells. Autoantibodies to Sm antigen, nuclear ribonucleoprotein complex and SS-B antigen were used. Although all three nonhistone antigens appeared to show speckled nuclear straining patterns in the Go phase, different patterns of staining were present at other periods of the cell cycle. The SS-B antigen showed a distinctly nucleolar localization during the G1/early S phase. These studies demonstrate that autoantibodies occurring in certain human diseases can be useful reagents for the immunohistological localization of nuclear macromolecules and for tracing their pathways during different phases of cell growth and differentiation.

Antibodies, Antinuclear↗
PubMed1981

[The correlation between autoantibodies and leukocyte migration index in myasthenia gravis (author's transl)].

Sera from 38 myasthenic patients and 15 non-myasthenic controls were surveyed by indirect immunofluorescence technique for reactivity against skeletal muscle, nuclei and mitochondria. Cell-mediated response to purified muscle antigen was detected by leukocyte migration inhibition assay. The positive rates for antiskeletal antibody (ASMA), antinuclear antibody (ANA), and antimitochondrial antibody (AMA) were 50%, 10.5% and 0%, respectively, in the myasthenic patients, and were 13.3%, 6.6% and 0%, respectively, in the controls. The mean titer of antiskeletal muscle antibody was 1:9.8 in the myasthenic patients and 1:1.8 in the controls. Both the positive rate and the mean titer of ASMA were higher and the migration index was lower in the patients with thymoma or thymic hyperplasia. To the contrary, among the group treated with corticosteroids, the correlation of the positive rate and mean titer of ASMA to migration index revealed a marginally significantly negative linear correlation (y = -0.72 chi + 84.69, r = -0.80, 0.1 greater than p greater than 0.05). This meant that the higher positive rate of ASMA was usually associated with the lower migration index. The facts of higher positive rate of ASMA in myasthenic patients and its negative correlation to migration index gave supportive evidence for a possible role of ASMA in the pathogenesis of myasthenia gravis.

Adolescent↗
PubMed1980

Impaired cell-mediated immunity function in thyroid cancer.

Immunological parameters were studied in 31 patients with thyroid cancer. Serum immunoglobulin levels, T and B cell percentages in peripheral blood, showed no significant difference as compared with those of control group. The blastogenesis with phytohemagglutinin P (PHA) was significantly reduced, especially, in those having larger tumor size or metastasis or in those over age 60. The positive rate of skin test with purified-protein-derivative (PPD) and Streptokinase-Streptodornase (SK-SD) was also markedly reduced. The impaired blastogenesis of lymphocyte and reduced positive rate of skin test in those cancer patients suggested that the cell-mediated immunity function, in spite of adequate count of T-cells, was impaired.

Adult↗