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Biomedical subjects

J S Hsieh

Publications and source records attributed to J S Hsieh.

At least 37 records · Page 2Linked to original sources
PubMed1995

A labile hyperphosphorylated c-Fos protein is induced in mouse fibroblast cells treated with a combination of phorbol ester and anti-tumor promoter curcumin.

Curcumin is a potent inhibitor of tumor promotion, and was shown previously to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced AP-1 activity. The c-Fos protein is inducible by TPA and thus is associated with c-Jun to result in an increased AP-1 activity in mouse fibroblast cells. We therefore hypothesized that c-Fos may be one of the targets of curcumin action. In the present study, the effects of curcumin on TPA-induced c-fos mRNA and protein levels were determined by RNA hybridization and western blot analysis, respectively. Curcumin decreases the TPA-induced nuclear abundance of c-Fos protein in spite of the slight super-induction of c-fos mRNA. Upon TPA stimulation, the amount of c-Fos in the quiescent cells increases and reaches maximum at 30 min, and then progressively disappears over a period of 60 min. However, the c-Fos protein seems susceptible to rapid degradation by 45 min if NIH 3T3 cells were treated with TPA in the presence of curcumin. The curcumin-induced hyperphosphorylated forms of c-Fos proteins are significantly more unstable; they entirely disappeared within 40 min after incubation at 37 degrees C. These findings prompted us to suggest that the decrease of c-Fos protein could account for the repressed in vitro DNA binding probably by reducing the Jun/Fos complex formation.

3T3 Cells↗
PubMed1995

Unusual sequences of group 3 LEA mRNA inducible by maturation or drying in soybean seeds.

Two cDNA clones, pGmPM8 and pGmPM10, which correspond to two mRNA species in mature or dry soybean seeds, were characterized. The deduced proteins, based on DNA sequence analysis, have a molecular mass of 49 and 51 kDa for pGmPM8 and pGmPM10, respectively. These two cDNA clones share a high homology with an amino acid identity of about 90% between the two deduced proteins. Both proteins appear to be extremely hydrophilic except at their N-termini that contain a 29 amino acid hydrophobic region at the N-terminus and the sizes of proteins decrease after co-incubating with ER membranes. These two proteins contain more than 30 similar, contiguous repeats of 11 amino acids, which is characteristic of group 3 LEA proteins. The mRNAs corresponding to pGmPM8 and pGmPM10 were expressed at high levels in dried or mature soybean seeds, but not in fresh immature seeds. The RNAs were also present in abscisic acid (ABA) treated leaves or cultured cells, and in tissues subjected to water stress or low temperatures.

Amino Acid Sequence↗
PubMed1995

A simple method of duct cannulation and localization for galactography before excision in patients with nipple discharge.

A monofilament polypropylene guiding suture was inserted into a secreting duct in 72 female patients (aged 15-78 years) with nipple discharge. Cannulation and injection of contrast material was successful in all patients before galactography and of methylene blue dye before excision. Patients did not report discomfort, no injuries ensued, and no duct was perforated. This technique should expedite excision of a discharging duct and any lobules.

Adolescent↗
PubMed1995

Frozen section of diagnosis of breast lesions.

Frozen section diagnosis rendered in 549 consecutive breast biopsies performed in 5 years in a single pathology laboratory was correlated with the final pathological diagnosis. There were no false positive reports among the 220 (40.1%) biopsies interpreted as benign lesions in paraffin sections. Among 329 (59.9%) malignant biopsies on paraffin sections, 3 cases were interpreted as benign lesions on frozen sections. Three false negatives included 2 ductal carcinoma in situ and one infiltrating ductal carcinoma associated with papillomatosis. The tumors were small and confined to the breast without any evidence of metastasis. There was a very good correspondence between the frozen section diagnosis and the paraffin section diagnosis (K = 0.98). The sensitivity of frozen section diagnosis was 99.1% and the clinical diagnostic specificity was 100%. Our results suggest that frozen section diagnosis is a highly reliable procedure, but small lesions (less than 1 cm in diameter, or non-palpable) should not be subjected to frozen section examination to avoid unnecessary loss of neoplastic tissue during the frozen section. The careful investigation of paraffin-embedded tissue is recommended for small breast lesions in breast conserving lumpectomy.

Breast Diseases↗
PubMed1995

Comparison of serum CA15-3 and CEA in breast cancer.

The monoclonal antibodies CA15-3 were developed against the two antigens 115D8 of the human milk fat globule membrane and DF3 of breast cancer. CA15-3 was assayed radioimmunologically and CEA was analysed using the enzyme immunoassay. Normal control was achieved in 32 healthy women, the mean values for CA15-3 were 11.5 +/- 3.0 u/ml, range from 7.9 to 16.9 u/ml. We compared serum levels of CA15-3 and CEA in 121 patients with histologically proved breast carcinoma. CA15-3 levels above 25 u/ml and CEA levels above 5 ng/ml were considered positive values. 31 of 121 patients studied had elevated CA15-3 levels (sensitivity: 25.6%) and 21 of 121 patients had positive CEA levels (sensitivity 17.4%). 92 of the breast cancer patients (76%) did not have metastatic disease. In this group CA15-3 sensitivity was 7.6%, while CEA sensitivity was 6.5%. Mean values were 15.1 +/- 6.6 u/ml for CA15-3 and 1.78 +/- 2.47 ng/ml for CEA. 29 patients (24%) had metastatic disease. In this group, CA15-3 sensitivity was 82.8% and CEA sensitivity was 51.7% (P < 0.05). Mean values for CA15-3 were 147.5 +/- 175.9 u/ml and 16.9 +/- 24.0 ng/ml of CEA. With regard to the correlation of two tumor markers with clinical course patients had significantly higher levels of CA15-3 than of CEA in metastatic breast cancer. This result suggests CA15-3 to be the more sensitive and more specific of the two for metastatic breast cancer detection and monitoring.

Adult↗
PubMed1994

Management of isolated gastric varices by devascularization and proximal gastrectomy in cirrhotic patients.

Twenty patients with liver cirrhosis were treated by surgery for bleeding from isolated gastric varices. The presence of tortuous and engorged gastric veins connecting with a large splenorenal shunt was demonstrated by transhepatic portography in all patients. The surgical procedures consisted of splenectomy, proximal gastrectomy, paragastric devascularization, and ligation of the splenorenal shunt. Sixteen patients survived the surgery. Four deaths were caused by emergency operation for uncontrollable hemorrhage in extremely poor risk patients. Of the 16 survivors, 15 had been followed with endoscopy and portography for a mean period of 42 months. The other one died of hepatocellular carcinoma three years after surgery. There was no bleeding episode during the period of follow-up in these patients. Recurrent esophageal varices of mild degree were documented by endoscopy and portography in three patients. Portography demonstrated that several newly formed retroperitoneal veins arising from the junction of the portal and superior mesenteric veins joined to form recurrent varices in these three patients. There was no significant change of the mean portal venous pressure before and after surgery. Our data reveals that elective surgery may provide satisfactory results in patients with isolated gastric varices. Transhepatic portography is the method of choice in radiologic investigation for prominent gastric varices.

Adult↗
PubMed1994

Abdominal wall hernia complicating continuous ambulatory peritoneal dialysis.

Continuous ambulatory peritoneal dialysis (CAPD) is a well-established treatment modality for end-stage renal disease. Abdominal wall hernia is not uncommon in patients on CAPD. Two factors are responsible for the occurrence of these hernias: anatomically weak sites and increased intraperitoneal pressure by the dialysate. Abdominal wall hernia was reviewed in 44 patients on CAPD from 1987 through 1991. Eight abdominal hernias (5 inguinal, 2 incisional and 1 umbilical) were found during CAPD treatment (18.18%). Surgical treatment was applied in 6 symptomatic hernias (5 inguinal and 1 incisional). After surgery, 5 patients returned to CAPD without recurrence and the remaining one died of strangulated incisional hernia. Our series shows that: (1) a detailed examination of the preexisting hernia and repair prior to CAPD treatment are suggested. (2) symptomatic abdominal wall hernia should be evaluated carefully and treated promptly. (3) awareness of the complication of these hernias can reduce the morbidity and mortality.

Adult↗
PubMed1994

Management of the perineal wounds following excision of the rectum for malignancy.

In a retrospective study, 103 patients undergoing abdominoperineal resection or total proctocolectomy due to rectal adenocarcinoma or familial polyposis with malignant change in the rectum were included. The methods for treating the pelvic and perineal wounds were divided into four categories: (1) suture of the pelvic peritoneum with open drainage, (2) suture of the pelvic peritoneum and perineum with simple drainage, (3) suture of the pelvic peritoneum and perineum with suction drainage, and (4) suture of the perineum with omental pedicle graft. The parameters analyzed were incidence of perineal wound infection, primary healing of the perineum, extraperineal complications and mean hospital stay. The infective rate of the group 1 was significantly higher than for the other groups. The incidence of primary wound healing for groups 2, 3, 4 was 73%, 78%, 81% respectively compared with group 1 of 20% at one month. There was no significant difference between the various groups regarding extraperineal complications. There was no significant difference in hospital stay among groups 2, 3, 4, but they were significantly shorter than for group 1. Primary closure of the pelvic peritoneum and perineal wound with simple drainage is now adopted by us due to its low infection rate, short hospital stay and easy performance.

Adult↗
PubMed1993

Enterolith ileus as a complication of duodenal diverticulosis--one case report and review of the literature.

Duodenal diverticulosis is not a rare condition. Usually of little clinical significance, it can produce a variety of disorders such as malabsorption, hemorrhage, diverticulitis, and obstruction. The rarest complication appears to be enterolith formation and obstruction. The case presented is a 70-year-old woman with the chief complaints of intermittent abdominal pain and vomiting. At laparotomy, duodenal diverticulitis and one enterolith obstructing the distal ileum were found. The literature review presents the other 26 cases with small bowel obstruction due to an enterolith formed within a small bowel diverticulum. The diagnosis can be established only by documenting the normalcy of the gallbladder and the presence of duodenal or jejunal diverticula.

Aged↗
PubMed1992

Dose-dependent kinetics of the enantiomers of MK-571, and LTD4-receptor antagonist.

The disposition of the enantiomers of MK-571 (MK-0679 and L-668,018) following single i.v. doses of MK-571 (L-660,711) was studied in a three way cross-over study in 12 healthy male volunteers. Each volunteer received 75 mg, 300 mg and 600 mg i.v. doses of MK-571 at weekly intervals. The disposition of both enantiomers appeared dose-dependent, since the AUC increased disproportionately faster than the dose. The dose dependency was much more pronounced for L-668,018: its AUC increased 6-fold from the 75 to the 300 mg dose, 16-fold from 75 to 600 mg and 2.7 fold from 300 to 600 mg. For MK-0679, the corresponding increases in AUC were 4.8-, 11-, and 2.3 fold. Regardless of dose, the elimination of L-668,018 was more rapid than that of MK-0679. The disposition of MK-0679 needs to be investigated independently to detect any potential influence of L-668,018 on its disposition.

Adult↗
PubMed1992

Mondor's disease of the breast--three cases report.

Mondor's disease is a rare and relatively little known clinical entity. Clinical features include sudden development of localized pain in the breast, followed by visible and papable, tender, cordlike, branching cutaneous grooves. It involves superficial thrombophlebitis of the thoracoepigastric or lateral thoracic vein occurring mostly in middle-age women. The etiology of Mondor's disease is unknown, and it may be related to trauma, operation, infection, bandaging, or tight clothes. The mammogram of most cases showed no abnormality, but the ultrasonography revealed hyperechoic cordlike pattern in the lesions. The symptomatic treatment is required and surgery will be indicated when it is associated with malignancy or severe painful cord due to pain and traction. It usually resolves over a period of 2 to 8 weeks. We report three cases of Mondor's disease and that we believe to be the first reported cases in Taiwan.

Adult↗
PubMed1990

Left paraduodenal hernia complicating pregnancy--a case report.

A pregnant woman with intestinal obstruction due to left paraduodenal hernia is described. To the authors' knowledge, this paper represents the first case of left paraduodenal hernia complicating pregnancy. Paraduodenal hernia is a congenital anomaly where the small intestine is trapped. The enlarging uterus distorts the hernial sac, and converts a partial obstruction into a complete one. The pathogenesis and management of this rare case is discussed.

Adult↗
PubMed1990

Clinicopathologic characteristics in colorectal adenocarcinoma and their relationship to survival.

In January 1981 at the surgical department of the Kaohsiung Medical College Hospital, we began a longterm clinicopathological study of colorectal adenocarcinoma according to "The General Rules for Clinical and Pathological Studies on Cancer of the Colon, Rectum and Anus" issued by the Japanese Research Society for Cancer of the Colon and Rectum. The aim was to evaluate this type of tumor's staging system as a guide to prognosis, and to determine the effects of several clinicopathological characteristics on survival. The data presented are based on the findings in the first 327 patients whose tumors were resectable. The clinicopathological variables included in this study were: age, sex, duration of symptoms, tumor location, the proportion of the bowel wall circumference involved, numbers of lymph nodes involved, depth of the cancer invasion, gross type, histological type and microscopic stage. The microscopic stage was found to be related strongly to survival. Other variables associated with significantly poorer five-year survival were: age under 40 years, duration of preoperative symptoms shorter than 6 months, a tumor occupying more than two-thirds of the bowel wall circumference, greater number of lymph nodes involved, more poorly differentiated cancer, the cancer invading into adjacent organs and ulceroinfiltrative tumor in univariate analysis. The overall five-year survival rate was 53.4% in this series.

Adenocarcinoma↗
PubMed1990

[Colon and rectal trauma].

From July 1982 to June 1989, 64 patients with trauma involving a colon and rectum operated on at the Kaohsiung Medical College Hospital were retrospectively reviewed. Their ages ranged from 14 to 90 years (mean 43.6 +/- 17.6 years); 48 were male, 16 female. Thirty-four (53.1%) had penetrating injuries, 30 (46.9%) had blunt injuries. Twenty-six (86.7%) blunt injuries and 7 (20.6%) penetrating injuries involved various associated injuries. There was a significantly greater number of associated organ injury in cases of blunt trauma. The most common organ injured concomitantly was the small intestine. The severity of injury and general condition were quantified for each patient using the intraoperative grading system proposed by Flint et al, and revealed that it was independent of the colorectal site, but related to the mode of trauma. After an assessment of the grade of injury, 42 patients underwent colostomies, 14 primary closures, 2 resections and anastomoses and 6 exteriorized repairs In the primary closure group, there was no mortality but 2 suture leakages which reoperated to colostomies. In three patients (50%) of the exteriorized repair group with fecal leakage developed at the repair site, the exteriorized loops were coverted into colostomies without complication. The most common complications were wound infection and abdominal abscess. The mortality (14.1%) and morbidity (45.3%) were not related to the location of the injured bowel but were influenced by the grade of colorectal trauma, associated organ injury and the mode of trauma.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1989

[Extent of the gastric antrum in duodenal ulcer patients].

The extent of the gastric antrum in duodenal ulcer patients and the relationship between the first antral branch of the vagal nerve and proximal delimitation of antrum were examined using resected gastric specimens of 46 duodenal ulcer patients. It has been demonstrated that: (1) There was a variable extent of measured distance from the pyloric ring to the antrum-corpus boundary (intermediate zone), and it ascended with age along the lesser curvature. (2) The intermediate zone was a transitional change of the fundic and pyloric gland area. The change on the lesser curvature was abrupt, but on the greater curvature it was gradual. It displaced distally on the greater curvature, and was found to be symmetrical on the anterior and posterior walls. (3) The proximal delimitation of the antrum lay on the lesser curvature where the first antral branch of the vagal nerve reached the lesser curvature topographically. (4) At the lesser curvature, the antrum-corpus boundary was found to be located within +/- 2 cm from the first antral branch in 93.5% of the cases. It may be a useful landmark to decide the proximal boundary of gastric antrum while performing antrectomy. (5) The shrinkage factor for specimens after 10% formalin fixation appeared to be between 0.7 and 0.9.

Adolescent↗
PubMed1989

Surgical treatment of bleeding and perforated peptic ulcer diseases.

From 1977 to 1988, 556 patients underwent emergency surgery for complicated peptic ulcers. Among them, 409 patients were for treated perforations and 147 for hemorrhages. In the perforated ulcer category, the morbidity rate was 12.5% and mortality rate 6.8%. For bleeding ulcers, the morbidity rate was 27.2% and mortality rate 14.9%. Several risk factors, such as age, interval of time between perforation and surgery, blood loss, associated disease, and shock were found to have created a higher operative risk. A definitive surgery including vagotomy and drainage, vagotomy and antrectomy, partial gastrectomy and highly selective vagotomy could be safely performed with an acceptable mortality for properly selected cases. Follow-up results also indicated better scores in this group of patients. We concluded that a definitive ulcer operation is safe, acceptable and curative for low risk patients with perforated or bleeding ulcers.

Adult↗