Biomedical subjects
Publications and source records attributed to J Töwe.
Administration of EAA and KA in patients with CRF on a protein-restricted diet led to a favourable influence on the plasma protein turnover. This was demonstrated by an increase in the half-life and a decrease in the breakdown rate of plasma proteins measured by the 15N tracer technique.
[15N]Glycine in a single oral dose was used to study nitrogen turnover in 18 short children, aged 3-14 yr. On the basis of their serum GH responses to insulin-induced hypoglycemia, the patients were divided into 3 groups: complete GH deficiency (GHD; n = 5); partial GH deficiency (pGHD; n = 6), and children with constitutional growth delay and familial short stature (CGD/FSS; n = 7). The mean 48-h renal excretion of 15N by patients with GHD was 66.09 +/- 14.12% (+/- SD) of the tracer dose. This decreased to 27.64 +/- 5.33% after two injections of 10 IU/m2 GH (P less than 0.001). 15N excretion by patients with pGHD was 47.19 +/- 13.42%, and it decreased after GH injection to 22.69 +/- 4.58% (P less than 0.005). Patients with CGD/FSS had 15N excretion of 37.27 +/- 5.68%, and it did not change in response to GH. The mean protein synthesis rate in GHD patients was extremely low, and it increased after GH injection from 0.99 +/- 0.46 to 3.53 +/- 0.43 g/kg X day. In pGHD patients the protein synthesis rate increased from 2.62 +/- 0.84 to 4.50 +/- 1.09 g/kg X day. The CGD/FSS patients had no change in protein synthesis rate after GH. Our results suggest that studies of the metabolism of [15N]glycine might be of value in predicting responsiveness to GH therapy.
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The action of prednisolone bisuccinate (Prednisolut) was tested on Wistar rats during two stages of prenatal development (embryonal development and early fetogenesis). Prednisolone application leads to phase-specific and dose-dependent disturbances in normogenesis (increased rate of resorption and retardation).
Three-factor associations in the HLA system are frequently tested statistically by well-known procedures using 2 X 2 X 2 contingency table analysis. The coupling index DABC given in many publications is suitable for none of these procedures. A more meaningful statistical test for the index DABC is described.
The theoretical bases and the performance of a sequential stimulation test (SST) with 0.5 g arginine hydrochloride per kg body weight, 25 micrograms gonadotropin-releasing hormone and 200 micrograms thyreotropin-releasing hormone are described. The result of SST is shown for 17 healthy women with biphasic cyclus. Beside the basal hormone levels B (hypo-, normo- or hyperhormonal) the response A (no, inadaquate or adaquate response) to the stimulation resulting from the peak levels and netto increases is a valuation criteria.
Protein synthesis and breakdown, nitrogen flux and other parameters of nitrogen metabolism were measured in five male preterm infants with a mean gestational age of 30.4 +/- 1.95 complete weeks of gestation and a mean body weight of 1592 +/- 517 g. The infants were fed on mothers' milk; the measurements were made at a post-conceptional age of 31.6 +/- 1.9 weeks of gestation (Ia) and were repeated at 34.4 +/- 1.9 weeks of gestation (Ib). [15N]-glycine (95 atom per cent) was used as a tracer, administered as a single enteral dose of 20 mg/kg. Whole-body protein parameters were calculated from an assumed three-pool model. The results were compared with data from moderately small preterm (II) and full-term (III) infants measured at post-conceptional ages of 36.1 +/- 1.4 and 48.0 +/- 2.8 weeks respectively. Protein synthesis rates tended to decrease with increasing post-conceptional age: 14.3 +/- 4.5 g/kg/d (Ia); 11.8 +/- 2.9 g/kg/d (Ib); 7.9 +/- 2.7 g/kg/d (II); and 7.7 +/- 1.4 g/kg/d (III). Protein breakdown and nitrogen flux showed the same trends. Possible consequences for the appropriate nutrition of very small preterm infants are discussed.
Clot determinations were done in plasma and in a mixture of amniotic fluid and plasma in relation 1:1 in 74 patients with toxemia of pregnancy and in 40 normal late pregnant women. Recalcification time was shortened. The activity of the factors of prothrobin complex was detectable in all normal and toxemic patients. Activity of factor V could be detected only in a few cases, plasminogen could not be deceted in the two groups. Clotting activity was stronger in patients with toxemia than in normal pregnant women.
The validity of using different 15N-tracer substances to measure whole body protein parameters, i.e., protein synthesis, protein breakdown, net protein gain, protein turnover, metabolic pool, and reutilization, was assessed by comparing the results obtained with: [15N]glycine, a mixture of 10 15N-labeled amino acids, and a 15N-labeled chicken egg protein in two infants, 9 and 12 weeks old, who were fed human milk. The tracer substances were fed orally as a single dose corresponding to a 15N-excess quantity of 0.2 mmol X kg-1 body weight. 15N Excretion in the urine was measured cumulatively by emission spectrometry, and the data on the protein metabolism were calculated by means of a three-pool model. All three tests yielded consistent net protein gains. The protein synthesis, protein breakdown, protein turnover, and nitrogen reutilization values produced by the [15N]glycine tracer study were higher than those produced by application of the 15N-amino acid mixture and the 15N-labeled egg protein. However, in our opinion, this discrepancy does not justify the replacement of [15N]glycine by expensive 15N-amino acid mixtures as tracer substances.
Protein synthesis, protein breakdown, protein-N turnover, and other parameters describing the nitrogen metabolism were measured in five male preterm infants. The weight of the subjects at birth was 2,064 +/- 107 g and the measurements were performed at age 16.0 +/- 4.5 days in the case of the mother's milk diet and 27.4 +/- 6.8 days in the case of the formula diet containing 1.8% protein. The parameters were measured by means of the 15N-tracer technique using [15N]glycine (95 atom %) applied in a single oral dose of 20 mg/kg as a tracer. The three-pool model proposed by Winkler and Faust was used to calculate the whole body protein parameters. No difference in net protein gain, protein synthesis, protein breakdown, or the other protein metabolism parameters were recorded despite the different protein inputs. Renal nitrogen excretion and the rate of endogenous urea N excretion were significantly higher for the formula diet than for the mother's milk diet. The protein synthesis rate of 7.9 g X kg-1 X day-1 was, as has previously been observed, higher than in other age groups. The protein metabolism of the preterm infant older than 33 weeks of gestational age does not benefit from a formula diet based on cow's milk that is richer in protein than mother's milk.
The paper deals with the increasing improvement of results obtained in treating acute lymphocytic leukemia in childhood on the basis of own patients (55 children admitted to hospital from 1969 to 1978). The assessment of the results is made by life table analysis according to the method of Cutler and Ederer. In the group of patients with a low risk more than half the children in stable first remission will exceed the 5-year limit after being diagnosed. In the group of patients with a high risk the results are significantly worse and require an intensification of the initial therapy step by step by using an aggressive initial stroke of treatment which is taken into consideration in the last protocol of therapy elaborated by the working committee for paediatric haematology and oncology in the paediatric society of GDR.
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A dose of 0.5 mg/kg body weight of indocyanine-green (Ujoviridin), a chromodiagnostic, was applied in one single injection under standardised conditions to 16 patients, aged between 21 and 31 years (25.9 years on average), with slight to moderate hyperemesis gravidarum, with the view to making an assessment of hepatic excretion.--Also determined were aminotransferases, alanine-aminopeptidase, and bilirubin in serum, accompanied by thymol turbidity tests.--The values of indocyanine-green half-life were all, but for one patient, within or at the upper limit of normal.--While the informative value of individual measurements is limited, the above findings seem to suggest that in cases of slight or moderate hyperemesis the laboratory parameters tested are unlikely to rise to pathological levels, except for slight increases which may be associated to certain individual cases.
There is reported about 2279 gynaecological operations at women, aged 60 years and more, in a period from 1960 to 1974, divided into three 5-years-groups. The part of geriatric operations increased from 5,3% to 7,0% of all operations. The division into high risk-groups took place after points given by Loskant. 41,1% of all patients where classified into group III. Group IV has the highest postoperative mortality rate (5,3%). The postoperative mortality rate altogether was 2,2%. In the years from 1960 to 1974 it decreased from 2,6% to 1,9%. 25,1% of the surgical operations where carried out abdominaly. The high part of vaginal operations comes off by placing curettages into the analysis. Under that aspect must be seen the postoperative mortality rate 7,1% at abdominal and 0,7% at vaginal operations. In the main point indications for operation were malignant tumours (37,3%), haemorrhages (25,4%), benign tumours (17,6%), and position variations of the genitals (10,6%).
20 fertile women who were treated with mestranol as oestrogen and both gestagenes chlormadinonacetate and norethisteronacetate were investigated. Alkaline phosphatase, ASAT, ALAT, alpha-amylase, cholesterol, bilirubin, serumproteins, indocyanine green half time, paper electrophoresis of serumproteins, thymol and zinc sulfate turbidity tests were examined during the first therapy cycle. Elevation of ALAT activity (6,3 I U/l to 12,14 u/l) was found during treatment with mestranol/chlormadinonacetate. A depression of ALAT (up to 5,34 u/l) was observed under additional therapy with gestagene chlormadinonacetate. Statistical significance was noted only for ALAT and alpha 1 globuline under sequential therapy with mestranol/norethisteronacetate.
Stain loading curves are analysed by discriminant analysis. In this multivariate statistical method, the stain concentrations measured in the blood at defined intervals after injection are regarded as measurement vectors.
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