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J Tada

Publications and source records attributed to J Tada.

At least 91 records · Page 5Linked to original sources
PubMed1992

Non-isotopic microtitre plate-based assay for detecting products of polymerase chain reaction amplification: application to detection of the tdh gene of Vibrio parahaemolyticus.

A non-isotopic microtitre plate-based assay method was devised for detection of products of the polymerase chain reaction. This assay involves affinity immobilization of the biotinylated amplification products in microtitre plate wells and their fluorescence detection by their hybridization with an oligonucleotide probe linked to alkaline phosphatase. An advantage of this procedure is that the immobilization and hybridization are carried out simultaneously in the wells, thus shortening the assay time. The assay method was applied to the detection of the tdh gene of Vibrio parahaemolyticus. Seven copies of the target chromosome could be detected in about 45 min after 35 cycles of amplification.

Alkaline Phosphatase↗
PubMed1991

Giant neuroendocrine (Merkel cell) carcinoma of the skin.

An 82-year-old woman had a dark red to purple tumor on the left buttock that had gradually enlarged during the last 5 years. Although routine histologic examination was not sufficient for diagnosis, neuroendocrine carcinoma was diagnosed by immunohistochemical and ultrastructural studies. Immunohistochemical-positive reactions to neurofilament, cytokeratin, neuron-specific enolase, and epithelial membrane antigen were noted. Electron microscopically, membrane-bound, dense core granules that yielded a positive uranaffin reaction and intermediate filaments in the perinuclear area were observed in the cytoplasm of most tumor cells. Desmosome-like structure between them was also found. Approximately 6 months after local excision, metastatic lesions developed in the regional lymph nodes and liver.

Actins↗
PubMed1991

Linear lupus erythematosus profundus in a child.

A 9-year-old Japanese boy had a 6-year history of a linear eruption of the left leg. It was characterized histopathologically by an intense lymphocytic panniculitis, perivascular and periappendageal infiltrates of lymphocytes, and vacuolization of the basal cell layer. This case represents a clinical presentation of linear lupus erythematosus profundus not previously reported.

Basement Membrane↗
PubMed1991

Distribution of low-density lipoprotein receptors and apolipoprotein B on normal and on reconstructed human epidermis.

Immunoelectronmicroscopy was employed to investigate the ultrastructural localization of the low density lipoprotein (LDL) receptor and the protein moiety of LDL (apo B) on normal human epidermis in situ and reconstructed human epidermis in vitro. For this purpose ultrathin sections of Lowicryl K4M embedded material were incubated with monoclonal antibodies against the LDL receptor and apo B followed by a second antibody conjugated to 15 nm colloidal gold. Examination of the sections revealed a similar distribution of the LDL receptor and apo B in normal human epidermis and in reconstructed epidermis. In both systems the amount of LDL receptors decreases during keratinocyte differentiation. In contrast, apo B molecules are more abundantly expressed in the upper layers of the stratum spinosum and the stratum granulosum. The great similarities in the distribution of the LDL receptor and apo B between the in vitro reconstructed epidermis and its in vivo counterpart provide additional proof that reconstructed epidermis is an excellent tool to investigate proliferation and differentiation processes of keratinocytes in vitro.

Apolipoproteins B↗
PubMed1991

Calcium deposition in the skin of a hemodialysis patient with widespread skin necrosis.

Rapidly progressive skin necrosis involving large areas of the skin associated with extensive vascular calcification was observed in a 39-year-old male patient on chronic hemodialysis. Histological examination of the periphery of a skin ulcer showed that subepidermal arterioles were occluded with fibrin deposits and that calcification was demonstrated in subcutaneous small arteries and arterioles. Electron microscopically, electron-dense materials, granular, crystal-like, vacuolar, or laminated, were found not only in arterial walls of the periphery of an ulcer but also, to a lesser extent, in those of normal-appearing skin. The skin necrosis gradually healed with antiseptic topical treatments and the injection of recombinant erythropoietin. The relationship between the vascular lesions in hemodialysis patients and progressive skin necrosis is not well known, although a combination of local and systemic factors may play an important role in the development of vascular calcification and skin necrosis in uremic and hemodialysis patients.

Adult↗
PubMed1991

Trigeminal trophic syndrome--a report of three patients.

Three Japanese patients with trigeminal trophic syndrome, a rare dermatosis in Japan, were reported. Cutaneous lesions were a long-standing ulcer and destruction of the right ala nasi in case 1, a persistent deep ulceration on the forehead after a small trauma in case 2, and development of small, discrete ulcers on the right forehead during the treatment of a postherpetic neuralgia in case 3. A protective device was very effective in one patient.

Aged↗
PubMed1991

[Punctate porokeratotic keratoderma].

A 39-year-old woman had numerous keratotic papules on the volar surface of the right palm, both soles, fingers and toes. There were some spiny plugs on the edges of her toes. Histologic examination revealed a cornoid lamella. "Punctate porokeratotic keratoderma" "porokeratosis punctata palmaris et plantaris" refer probably to related conditions "keratosis punctate palmaris et plantaris". We prefer the term "punctate porokeratotic keratoderma".

Adult↗
PubMed1990

Differences in low density lipoprotein receptor expression in the suprabasal layer of normal and psoriatic epidermis.

Previous morphological experiments on the distribution of binding sites for low density lipoprotein (LDL) on normal and psoriatic epidermis in situ, done with the LDL-gold technique [Mommaas-Kienhuis AM, et al. J Invest Dermatol 89: 513-517, 1987.] showed an unequivocal correlation between the ability to bind LDL-gold complexes and the state of keratinocyte differentiation. To determine the involvement of the LDL receptor in this phenomenon, we applied immunoelectronmicroscopical methods in conjunction with a monoclonal anti-LDL receptor antibody. Biopsy specimens of normal and psoriasis skin were fixed before being embedded in Lowicryl K4M. Ultrathin sections were incubated first with the anti-LDL receptor antibody, and then with a second antibody conjugated to colloidal gold. On basal cells of both normal and psoriatic epidermis the LDL receptor was distributed evenly between the cell surface and the cytoplasm. No obvious differences in the density of LDL receptors were observed. However, cells from the suprabasal layer showed two striking differences in the localization of the LDL receptor: 1) normal epidermis showed fewer LDL receptor molecules, whereas in psoriasis epidermis the number increased relative to those on basal cells; and 2) in normal suprabasal cells most of the LDL receptors were located inside the cell, but in psoriasis the majority was found on the cell surface. Both phenomena are discussed and we postulate that the higher expression of LDL receptors in psoriasis suprabasal cells and the high expression of the receptor on the cell surface is connected with the hyperproliferative state of the disorder.

Humans↗
PubMed1990

[Herpes simplex virus type I encephalitis with left temporal lobe lesion detected by gadolinium enhanced MRI].

A case of herpes simplex virus (type I, HSV-I) encephalitis (HSE) was reported. A 65 years old man presented acute fever and subsequently developed mental symptoms, disorientation and consciousness disturbance. HSV-I antibody titers of serum and CSF were markedly elevated, especially the elevation of IgM antibody was noticeable. MRI T1 weighted image enhanced by Gadolinium DTPA taken on the 15th day from the onset revealed a lesion in the inner side of left temporal lobe. Acyclovir therapy was administered under the diagnosis of HSE. He recovered well with the residual of moderate memory disturbance. This is a very interesting and rare case of HSE in an old patient caused by the primary infection of HSV-I. In addition, the importance of Gd enhanced MRI study to detect HSE lesion was discussed.

Aged↗
PubMed1990

[Angiolymphoid hyperplasia with eosinophilia--report of a case with reference to the vascular changes and cell infiltrates in this disease].

We reported angiolymphoid hyperplasia with eosinophilia (ALHE) in a 47-year-old woman. The most characteristic histological findings were epithelioid endothelial cells with intracytoplasmic vacuoles, and remarkable arterio-venous (A-V) shunts. Mast cells, seen in the lesions, had no special relationship with eosinophils in their distribution. Degranulation of mast cells was not seen. Electron microscopic study revealed abundant cytoplasmic organellae and fine filaments in epithelioid endothelial cells. Immunohistochemical study revealed mixed infiltration of T and B lymphocytes, and polymorphous T-cell population in the lesions. These findings suggest that active vascular proliferations take place in the lesions of ALHE, that the eosinophilic infiltrate and mast cells may be independent of each other, and that the lymphocytic infiltrate probably is a reactive process. The vascular changes seen in Kimura's disease are characterized by a proliferation of capillaries with swollen, but not epithelioid, endothelial cells and vessels with lamellated adventitia. A-V shunts are not observed in its lesions. Therefore ALHE and Kimura's disease should be considered different entities.

Angiolymphoid Hyperplasia with Eosinophilia↗
PubMed1989

Highly sensitive detection of DNA using enzyme-linked DNA-probe. 1. Colorimetric and fluorometric detection.

In order to develop non-radioactive oligonucleotide derivatives and to examine their utility as a diagnostic tool, namely as DNA-probe, an enzyme-linked oligonucleotide was synthesized. Oligonucleotide complementary to M13mp8 phage DNA was linked to alkaline phosphatase via a crosslinker and a spacer. M13mp8 phage DNA (single strand) immobilized on the nitrocellulose membrane was hybridized with the enzyme-linked oligonucleotide. The hybrid was detected with three detection methods; (1)colorimetric detection in solution, (2)colorimetric one on membranes, and (3)fluorometric one in solution. Methods(2) and (3) gave high sensitivities to detect as low as several to several tens attomoles of DNA and it was found that those methods with enzyme-linked oligonucleotides are potent for DNA-probe methodology from the viewpoint of automation.

Chromatography, Gel↗
PubMed1989

Two sisters with impetigo herpetiformis.

Two sisters (non-twins) had impetigo herpetiformis triggered by their first pregnancy. One sister treated with etretinate had good resolution of skin disease. Impetigo herpetiformis is probably a variant of pustular psoriasis and may be provoked or precipitated by factors, such as pregnancy. Both patients shared common HLA antigens (All, AW24, BW44, BW54 and DR6Y).

Adult↗
PubMed1988

[A case of HTLV-I associated myelopathy (HAM) complicated by mononeuritis multiplex].

A 42-year-old woman with progressive myelopathy and mononeuritis multiplex is reported. The neurological examination on admission revealed hyperreflexia of the four extremities with pathological reflexes and moderate muscle weakness of the lower extremities with spasticity. Sensory disturbance was distributed on the areas of the bilateral lateral cutaneous femoral, the superficial peroneal and sural nerves. The antibody to HTLV-I in the serum and cerebrospinal fluid was more than 8192X and 512X, respectively. No sensory potential was recorded in the sensory conduction study of bilateral lateral femoral cutaneous nerves. Corticosteroid therapy caused a marked improvement of the sensory and urinary disturbances and had a slight effect on the spastic gait. Our nerve conduction study found small sensory potentials with normal conduction velocities in the bilateral lateral femoral cutaneous nerves. These results suggested the presence of an axonal degeneration in the peripheral nerves in this case. There have been no reports in the literature regarding a case of HAM with mononeuritis multiplex.

Adult↗