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Biomedical subjects

J Tanaka

Publications and source records attributed to J Tanaka.

At least 865 records · Page 48Linked to original sources
PubMed1980

Stimulltion of mitochondrial phosphorylative activity in the regenerating rabbit liver following oral antibiotic treatment.

In this investigation, oral Kanamycin was administered to young male rabbits for 3 days, the control group receiving only water. Partial hepatectomy was then performed in all of them, and mitochondrial activity, energy charge and portal vein ammonia were measured at 12 and 24 h postoperatively. It was found that mitochondria from oral Kanamycin-treated rabbits have higher respiratory control ratios, higher state 3 respiration and higher P/O ratios than the control group, suggesting a further enhancement of phosphorylative activity. Energy charge in the Kanamycin-treated group showed less acute changes suggesting a positive balance in the energy status of those livers. The ammonia level here was much lower than in the control group. These findings suggest that the removal or the decrease of the overloading effect of ammonia resulting from bacterial activities in the intestine, has a beneficial effect on the healing process in liver surgery.

Adenine Nucleotides↗
PubMed1980

Relationship between tumor formation and cell-mediated immunity in hamsters with transplanted HVJ (Sendai virus)-carrying tumor cells.

Using macrophage migration inhibition (MMI) and the cell-mediated cytotoxicity (CMC) test, the cell-mediated immune response in hamsters with transplanted HVJ-carrying tumor cells (THEL-HVJ or THEL-HVJpits) was examined in relation to the lowered transplantability of the cells. The cells cultured at a temperature (34 degrees) permissive for HVJpits (temperature-sensitive HVJ) showed significantly lowered transplantability in hamsters. After shifting the cell culture temperature up to 39 degrees (non-permissive for HVJpits) for 5 days, THEL-HVJpits cells which had lost their cellular HVJ antigens regained the same high transplantability observed in parent THEl cells. However, culture of the cells at 37 degrees (partially permissive) for 24 hr did not significantly affect their tumor-forming ability with lowered transplantability, in spite of a considerable reduction in cellular HVJ antigens. The MMI test on hamsters with transplanted THEL-HVJ or THEL-HVJpits cells cultured at 34 degrees (MMI/THEL-HVJ 24 or MMI/THEL-HVJpits 34) was more markedly positive, and for a longer period (1 to 4 weeks), than the same test on hamsters inoculated with THEL cells. However, the MMI/THEL-HVJpits 39 cells acquired lowered reactivity like those from THEL-tumor bearing animals, while MMI/THEL-HVJpits 37 cells were still positive, just as in MMI/THEL-HVJpits 34. In the CMC test, much more cytotoxic activity was observed in spleen cells from hamsters with transplanted THEL-HVJpits cells than in those from THEL-transplaned animals; there was a general correspondence with the results obtained in the above MMI test. These findings strongly indicate that the lowered transplantability of HVJ-carrying tumor cells may be due to a significant induction of cell-mediated immune responses. It is suggested that cell membrane antigens modified (xenogenized) by the complete or partial expression of HVJ genomes carried may play an important part in this induction in vivo.

Animals↗
PubMed1980

Time-course of mitochondrial and cytoplasmic oxidoreduction state of rat liver following an intravenous glucose load.

Changes in mitochondrial and cytoplasmic oxidoreduction states after an intravenous glucose infusion were studied in the freeze-clamped liver of normal and alloxan-induced diabetic rats. In normal rats the mitochondrial oxidoreduction state (NAD+/NADH) increased maximally at 5 min after glucose infusion (p less than 0.001) and then returned to about normal fasting level at 30 min later. The cytoplasmic oxidoreduction state reached its peak level at 30 min after glucose infusion (p less than 0.001). A clear temporal correlation was observed between the mitochondrial oxidoreduction state and insulin and glucose levels in plasm. On the other hand, in alloxan-induced diabetic rats no changes were detected in the oxidoreduction state os either mitochondria or cytoplasm in response to glucose infusion. Therefore the increase in portal insulin level as it occurs in response to the rise of blood glucose may play a role in the shift of the mitochondrial oxidoreduction state to its more oxidized state. This shift seems likely to be due to an accelerated removal of the reducing equivalents through electron transfer by the respiratory chain.

Animals↗
PubMed1980

[The effect of 1 alpha-OH-D3 on the process of fracture healing (author's transl)].

The purpose of this paper is to clarify the effect of 1 alpha-OH-D3 on the bone metabolism through fracture healing. Rats were fractured on the middle of femur and were given 1 alpha-OH-D3 every day. The relationship between biochemical and histological changes were studied through the process of fracture healing associated with remarkable remodeling. The results are summarized as follows: 1. As soon as serum Ca levels decreased temporarily after fracture, a increase of serum iP.T.H. levels occurred with the histological change such as a bone resorption. The secretion of P.T.H. is probably suppressed by 1 alpha-OH-D3 with subsequent increase of the resorption of bone tissue. 2. Following the increase of serum Ca levels, the decrease of serum iP.T.H. levels were observed, accompanied by the histological changes of bone formation. These changes were seen earlier in the 1 alpha-OH-D3 group than in the controlled group. 3. Bone formation in the 1 alpha-OH-D3 group could be seen at the same area at which the bone resorption had occurred before. 1 alpha-OH-D3 could promote osteocytic, osteoclastic osteolysis and also bone formation, activating basic multicellular units (B.M.U.). 4. Some difference was found in the uniting callus formation between 1 alpha-OH-D3 group and the controlled group. 5. In conclusion, administration of 1 alpha-OH-D3 during fracture healing is useful in stimulating direct callus formation from cortex.

Alkaline Phosphatase↗
PubMed1980

Predisposing factors of renal dysfunction following total correction of tetralogy of Fallot in the adult.

A retrospective study of 21 adult patients with tetralogy of Fallot (TOF) was undertaken to determine the predisposing risk factors of renal dysfunction following total correction of the disease. Five of the 21 patients exhibited moderate-to-severe postoperative azotemia and an additional five exhibited mild azotemia. Significant risk factors for postoperative renal dysfunction found in routine preoperative examinations were as follows: arterial oxygen saturation of less than 90%, cardiothoracic ratio (CTR) of greater than 50%, mean electrical axis of the QRS complexes of greater than +120 degrees, S wave in Lead V6 of greater than 7 mm, R/S voltage ratio in Lead V6 of less than 2, and negative T wave in Lead V6. In addition, preoperative cardiac catheterization data showed that the patients exhibiting postoperative azotemia had more severe pulmonary stenosis, a smaller pulmonary-to-systemic flow ratio (Qp/Qs), and a larger left ventricular cavity than nonazotemic patients. The incidence of postoperative low cardiac output state (LOS) was significantly higher in the azotemic patients. These findings suggest that a combination of the severe form of TOF and a large left ventricle increase susceptibility to LOS and postoperative renal dysfunction. The cause and the clinical significance of the large left ventricular cavity in adults with TOF are discussed.

Acute Kidney Injury↗
PubMed1979

Early metabolic disturbances in the liver following unilateral hepatic or common bile duct obstruction in rabbits.

The early metabolic disturbances in the liver following ligation of the left hepatic or the common duct were studied in the rabbit. In the rabbits subjected to ligation of the left hepatic bile duct, the phosphorylative activity of the liver mitochondria in the ligated lobe decreased to about 50% of controls 48 h after the ligation, while that in the nonligated lobe increased 2-fold. These rabbits continued to live. On the other hand, in the rabbits subjected to ligation of the common bile duct, the mitochondrial phosphorylative activity increased slightly at 3 h after the ligation but fell rapidly thereafter. The mitochondrial free NAD+ to NADH ratios and hepatic energy charge (ATP+1/2ADP/ATP+ADP+AMP) markedly decreased after the ligation. Most of these rabbits did not survive over 2 days. These results indicate that the metabolic disturbances in the jaundiced liver are due to an impairment of the oxidative phosphorylation. In addition, it is suggested that the maintenance of the energy balance by enhanced mitochondrial phosphorylative activity is readily impaired in the jaundiced liver.

Adenine Nucleotides↗
PubMed1979

Studies on the immunopotentiating effects of a streptococcal preparation, OK-432. I. Enhancement of T cell-mediated immune responses of mice.

The effects of the anti-tumour agent OK-432 on the immune response to hamster erythrocytes (HRBC) and nucleated chicken erythrocytes (CRBC) were studied in inbred SL mice. Mice were treated repeatedly with OK-432 before immunization with erythrocytes in saline. The cytotoxicity of CRBC-primed spleen cells, as demonstrated by 51Cr release from labelled CRBC, was markedly increased by treatment with OD-432. The delayed footpad reaction to CRBC was significantly augmented by treatment with OK-432. These results in mice indicate that OK-432 can enhance the cellular immune responses which require the contribution of T cells. Such an activation of T cells by OK-432 was observed in the humoral immune response to a trinitrophenyl group. Augmentation of anti-hapten antibody production, suggesting the enhancement of helper T cell activity by OK-432, was noticed after immunization with trinitrophenyl conjugated to erythrocytes. Furthermore, this enhancement of helper T cell activity by OK-432 was confirmed by utilizing an adoptive transfer system. These results support the possibility that T cell activation may be one of the important effects of OK-432 as an immunopotentiator.

Adjuvants, Immunologic↗
PubMed1979

Significance of blood ketone body ratio as an indicator of hepatic cellular energy status in jaundiced rabbits.

Changes in the free NAD+/NADH ratio and the energy charge (ATP + 1/2 ADP/ATP + ADP + AMP) of the liver were compared with the ratio of acetoacetate to beta-hydroxybutyrate of arterial blood in rabbits subjected to ligation of the common bile duct. Both the acetoacetate/beta-hydroxybutyrate ratio of the liver, which reflects mitochondrial free NAD+/NADH ratio, and the heptic energy charge decreased in accordance with the decrease of mitochondrial phosphorylative activity after the ligation. The decrease in the acetoacetate/beta-hydroxybutyrate ratio of the liver was attributed to a restricted mitochondrial reoxidation of NADH due to an inhibition of oxidative phosphorylation. Moreover, changes in the ratio of acetoacetate to beta-hydroxybutyrate in arterial blood were positively correlated with those of the liver (r = 0.695, P less than 0.01) and the hepatic energy charge (r = 0.844, P less than 0.01). It is suggested that the ratio of acetoacetate to beta-hydroxybutyrate in blood can reflect the energy charge of the liver in jaundiced rabbits.

Acetoacetates↗
PubMed1979

Ascaris and eosinophil. II. Isolation and characterization of eosinophil chemotactic factor and neutrophil chemotactic factor of parasite in Ascaris antigen.

In the previous study, we found an eosinophil chemotactic factor of the parasite (ECF-P) in extracts from the body cavity fluid of the Ascaris species. In this study, the physiochemical characteristics of ECF-P were determined in order to elucidate the relationship between ECF-P and Ascaris antigens. ECF-P was active in both in vivo and in vitro chemotactic assay systems and was found to be heat labile, nondialyzable, and stable to lyophilization. The estimated m.w. was approximately 30,000 daltons. The isoelectric point of ECF-P was 8.5. It was quite different from the Ascaris antigen in the same extract, or various Ascaris allergens that had been reported previously by other researchers. Furthermore, ECF-P did not show any activity in eliciting a guinea pig passive cutaneous anaphylaxis (PCA) reaction. From these experimental results, we concluded that ECF-P is not identical to Ascaris antigens. In addition, we found that the neutrophil chemotactic factor of the parasite (NCF-P), with at least two components, is present in the Ascaris extract, and is also heat labile and resistant to dialysis and lyophilization, but separable from coexistent ECF-P by reason of their different physiochemical properties.

Animals↗
PubMed1978

Bilateral necrotizing scleritis.

A 53-year-old woman whose history was noncontributory for rheumatoid arthritis and collagen disease had bilateral necrotizing inflammation of the sclera. The left eye had necrotizing nodular scleritis and the right eye, massive granuloma of the sclera. In the right eye the orbital adnexae were also involved by granulomatous infiltration and developed a mass of fibroadipose tissue simulating an orbital tumor within a week after enucleation.

Conjunctiva↗
PubMed1978

Effect of double infection of cowpox virus-infected cells with paramyxovirus (Sendai virus) on formation of cowpox virus-specific cell surface antigen.

The formation of cowpox virus-specific cell surface antigen (CPV S-ag) was significantly enhanced by double infection with HVJ (Sendai virus). Simultaneous double infection, superinfection with HVJ and superinfection with CPV of cells persistently infected with HVJ similarly enhanced the formation of CPV S-ag, while pre-infection with HVJ was ineffective. To be effective, cells must be infected at a m.o.i. of greater than or equal to 1.0 and HVJ gene functions had to be expressed. The HVJ-infected cell extracts had an ability to accelerate uncoating (or degradation) of CPV, causing an early increase and a subsequent decrease in the infectivity of CPV. This activity reached a maximum 4--6 hr after HVJ infection, the increase paralleling enhancement of the total activity of several cellular enzymes. Addition of puromycin abolished the increase of these activities and the formation of CPV S-ag. Thus, the double infection with HVJ of CPV-infected cells induces an enhancement of CPV S-ag formation presumably as a consequence of activation of cellular enzymes which in turn accelerates uncoating of CPV.

Antigens, Surface↗
PubMed1978

Acute thallotoxicosis: neuropathological and spectrophotometric studies on an autopsy case.

Acute thallotoxicosis has been studied in an autopsy case with characteristic neurological symptoms. Pathologic findings were composed of a rarefaction in cerebral white matter, swelling and fragmentation in peripheral nerve fibers and some chromatolytic changes in neurons of cranial nerves and spinal cord. Trace amounts of thallous salt were measured in the tissues from the brain, liver and kidney using spectrophotometric technique. The locus of thallium affection is assumed to be a mitochondrial membrane with a specific affinity to the thallous ion.

Adult↗
PubMed1978

Immunological studies on myasthenia gravis: operative indication and cell-mediated immunity.

An important aspect of the management of patients with myasthenia gravis is the decision to recommend thymectomy. Hitherto, many investigators have reported the relationship between the operative effects and such factors as age, sex, duration of symptoms, or degree of germinal center proliferation in the myasthenic thymus. However, these reports are not practical aids in deciding the indication for thymectomy in an individual myasthenic patient. The currently accepted indications of thymectomy for myasthenic patients are (1) the thymomatous patient, especially those with malignancy, and (2) the nonthymomatous patients who are resistant to medical treatment. From our present data we would add the following as an indication of the operation: (3) patients who have high T-cell subpopulation levels with highly blastogenic activities and strong skin test reactivities. In order to assure good operative results in myasthenic patients, surgeons should examine their patients' preoperative immunological states.

Adolescent↗
PubMed1978

Anisakis and eosinophil. I. Detection of a soluble factor selectively chemotactic for eosinophils in the extract from Anisakis larvae.

Studies were undertaken in order to determine if Anisakis larva itself has the ability to attract eosinophils. Extracts from Anisakis larvae were examined for both neutrophil and eosinophil chemotactic activities with both in vivo and in vitro assay systems. When the soluble extract was injected intradermally into normal guinea pigs, a profound accumulation of eosinophils was observed at the injection site. The cells started to accumulate at the site within 1 hr and the number of eosinophils at the site reached its peak at 8 hr after the injection of the extract. Such eosinophilic accumulation was enhanced in a dose-response fashion over the range of 0.5 to 50 micrograms protein. The strong chemotatic activity of the Anisakis extract for eosinophils was confirmed when in vitro chemotaxis assays were performed with Boyden chemotatic chambers. Interestingly, no chemotactic activity for neutrophils was found at those concentrations of the extract with which the eosinophil effect was observed. These results indicate that the factor described here, in addition to the various known immunologic factors, may play an important role in the development of eosinophilia in anisakiasis.

Animals↗