Biomedical subjects
Publications and source records attributed to J W Chin.
We describe cellular engineering for the creation of multiple new cellular modules each composed of an orthogonal ribosome and orthogonal mRNA. These modules operate independently of the endogenous ribosome and mRNA. We discuss some of the applications of orthogonal pairs and highlight the expression of Boolean logic in gene regulation using multiple orthogonal pairs.
Synthetic genes for avian pancreatic polypeptide (aPP) and for the miniature DNA binding protein PPBR4 were cloned and expressed on the surface of M13 bacteriophage. We anticipate that these constructs will have utility optimizing the properties of miniature proteins based on aPP that result from our previously described protein grafting procedure.
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A new way by which the potency of a eukaryotic transcription factor can be regulated has been discovered, in which nuclear factors increase the concentration of the transcription factor's active form by modulating an otherwise unfavorable equilibrium between monomeric and dimeric forms of the protein.