Methodology for optimizing functional miniature proteins based on avian pancreatic polypeptide using phage display.

J W Chin; R M Grotzfeld; M A Fabian; A Schepartz

doi:10.1016/s0960-894x(01)00139-1

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Methodology for optimizing functional miniature proteins based on avian pancreatic polypeptide using phage display.

J W Chin·R M Grotzfeld·M A Fabian·A Schepartz

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Abstract

Synthetic genes for avian pancreatic polypeptide (aPP) and for the miniature DNA binding protein PPBR4 were cloned and expressed on the surface of M13 bacteriophage. We anticipate that these constructs will have utility optimizing the properties of miniature proteins based on aPP that result from our previously described protein grafting procedure.

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Animals Bacteriophage M13 Cloning, Molecular DNA-Binding Proteins Drug Design Methods Pancreatic Polypeptide Peptide Fragments Peptide Library Protein Structure, Secondary Structure-Activity Relationship Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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J W Chin, R M Grotzfeld, M A Fabian, A Schepartz. 2001-06-18. Methodology for optimizing functional miniature proteins based on avian pancreatic polypeptide using phage display.. https://doi.org/10.1016/s0960-894x(01)00139-1

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