Biomedical subjects

J Wiśniewski

Publications and source records attributed to J Wiśniewski.

At least 19 recordsLinked to original sources

PubMed2000

Insertional knock-out of protein translocation systems common for Yersinia enterocolitica and Listeria monocytogenes.

To carry out efficient insertional mutagenesis in Listeria monocytogenes and to facilitate the characterisation of disrupted genes, a novel derivative of plasmid pACYC 184 was constructed, pLIV virA3, carrying a fragment from the virA region of the of Y. enterocolitica plasmid pYVe 0:9. After transformation of this plasmid into L. monocytogenes it was possible to select for its integration into the host DNA at 42 degrees C. Insertional mutants of L. monocytogenes obtained by using pLIV vector containing plasmid DNA fragments from Y. enterocolitica were constructed and are described.

Bacterial Proteins↗

PubMed2000

[Single use insulin injector evaluated by patients (questionnaire study)].

The aim of the study was the analysis of perception of the disposable pen injector HumaJect by diabetic patients. Selected features of the insulin delivery systems were evaluated, and on that basis, the comparison between HumaJect and other insulin injectors was made. Research material was collected in questionnaires filled out by doctors after interviewing patients who were using HumaJect for at least one month. 1802 diabetic individuals aged 9 to 88 participated in the study. HumaJect was ranked "very good" (which is the highest possible rank) by 71% of patients. Among specific features, the highest ranks were assigned to "Ease of dose setting", "Dosing range" and "Disposable form". Most of patients assigned higher ranks to HumaJect than to other insulin delivery systems. 89% of patients indicated desire to continue treatment with this injector.

Adolescent↗

PubMed1994

Cloning, nucleotide sequence and expression of rat heat inducible hsp70 gene.

In rat cells hyperthermia induces two hsp70 transcripts of 2.5 kb and 2.7 kb. We have cloned and determined the nucleotide sequence of a gene (named hsp70.1) encoding the 2.5 kb transcript as shown by Northern blot analysis using the 5' end and 3' end specific hybridization probes. It contains an uninterrupted open reading frame of 1926 bp, it encodes a protein of approx. 70,100 Da and the predicted amino acid sequence of its product shows 98% similarity to the mouse hsp70.1 protein. The transcription start site was localized 224 bp upstream the ATG codon by RNase protection and primer extension mapping. Upstream the transcription initiation site several potential regulatory motifs including a TATA box, two Sp1 binding sites, one inverted and one direct CCAAT box and three HSEs (heat shock elements) were found. Transfection experiments with constructs in which the CAT reporter gene was fused to fragments of the 5' end flanking sequences of the isolated gene confirmed that the promoter of the rat hsp70.1 gene is functional and heat inducible.

Albinism↗

PubMed1994

Lack of correlation between the presence of 70 kb plasmid and plasmid-associated determinants of Yersinia enterocolitica.

The relatedness between the plasmids from Yersinia enterocolitica 0:4.32 and 0.9 was examined. Plasmid 0:4.32 was found in many clinical isolates of Y. enterocolitica and was related to pYV plasmid of Y. enterocolitica 0:9. The homology between the plasmid and chromosomal DNAs of strains 0:4.32 and 0:9 was examined. The Ca(2+)-dependent expression of the OmpF and OmpC proteins is described.

Animals↗

PubMed1993

An intradermal test for the diagnosis of BHV-1 infection. The effect of repeated testing on the immune status of cattle.

The effect of the repeated delayed-type hypersensitivity (DTH) testing (an intradermal test--ID) on virus neutralizing (VN) antibody and DTH responses has been studied. Repeated intradermal injection of inactivated BHV-1 antigen elicited neither the virus neutralizing antibody nor positive skin reaction in cattle free from BHV-1 infection. On the other, hand, the same manipulation carried out in a herd infected with BHV-1 provoked seroconversion and/or positive DTH reaction in some of the animals without detectable pre-existing VN antibody. Cattle initially positive by VN and intradermal tests showed steady increase in antibody titre, and some of them developed little or no skin reaction following repeated DTH testing.

Animals↗

PubMed1993

An evaluation of an intradermal test for the diagnosis of bovine herpesvirus type 1 (BHV-1) infection in cattle.

An intradermal (ID) test (a delayed-type hypersensitivity test) was used for the diagnosis of BHV-1 infection in cattle. A threshold value for the test positive results was established based on the analysis of the agreement between the results of ID test and those of virus neutralization (VN24) assay. Linear regression analysis revealed no correlation between the intensity of the skin reaction (expressed as an increase in skin-fold thickness) and the VN24 antibody titre. The sensitivity and specificity of the ID test compared to VN24 one, were 86.6% and 98.3% respectively. Of the 487 cattle with an age of over 6 months, 25 (5.1%) reacted discordantly by ID and VN24 tests, i.e. 19 seropositive animals expressed little or no skin reaction, whereas 6 seronegative individuals were positive by ID. Neither of the calves, with passively acquired antibodies in contrast to experimentally infected ones, showed distinctive skin reaction.

Animals↗

PubMed1991

The effect of some physical and serological treatments on haemofusing activity of bovine parainfluenza virus type 3.

Bovine parainfluenza virus type 3 irrespective of the time of its harvesting from Madin-Darby bovine kidney cells, expressed little or no haemofusing activity. Treatment of the virus by freezing and thawing, sonication or antibody and complement enhanced this activity. Moreover haemofusing activity did not correlate with the viral capacity for fusion of susceptible cells in monolayer cultures.

Animals↗

PubMed1990

Isolation and nucleotide sequence analysis of the rat testis-specific major heat-shock protein (HSP70)-related gene.

The isolation of a rat hsp 70-related gene which is specifically and highly expressed in testis is described together with the complete nucleotide sequence of the transcription unit (2947 bp), 5' flanking (about 1 kbp) and 3' flanking (about 0.3 kbp) regions. The sequence analysis and nuclease S1 mapping revealed that the isolated gene (referred to as the hst70 gene) represents a novel, distinct member of the hsp70 multigene family. Its transcription unit lacks introns and a single open reading frame encodes a protein of 69.5 kDa. The predicted amino acid sequence of this protein is highly similar (only four out of 633 amino acids are different) to that encoded by the mouse testis-specific hsp70.2 gene (Zakeri, Z.F., Wolgemuth, D.J. and Hunt, C.R. (1988) Mol. Cell. Biol. 8, 2925-2932). The functional significance of multiple potentially regulatory sequences (e.g. TATA-boxes, heat-shock element and estrogen receptor binding site) present in the 5' flanking region of the rat hst70 gene is discussed.

Amino Acid Sequence↗

PubMed1990

Hemagglutination by herpes simplex virus type 1.

The McIntyre and HSZP strains as well as clinical isolate of herpes simplex virus type 1 were found to agglutinate C57Bl/10su and CBA mouse red blood cells. The hemagglutinating activity was inhibited by antisera that neutralized the infectivity of the virus.

Animals↗

PubMed1990

Isolation of a gene coding for a major heat shock protein HSP71 in rat.

A rat gene closely related to a human heat-inducible/cell cycle-dependent hsx70 gene was cloned from a rat genomic library. Northern blot analysis showed that it encodes a 2.5 kb transcript, one of the two heat-inducible mRNAs (2.5 and 2.7 kb) detected in rat tissues. Comparison of the known expression pattern of rat major heat shock proteins and mRNAs suggests that the isolated rat gene most probably codes for HSP71 protein.

Animals↗

PubMed1989

Activation of the glucose-regulated gene (grp78) in regenerating rat liver is nonspecific and is related to acute phase response.

The expression pattern of the hsp70 gene family during regeneration or rat liver has been investigated. Northern blots were prepared from total RNA isolated from livers at 0 h (control), 12 h (end of prereplication phase), 24 h (maximum of DNA synthesis) and 36 h (postmitotic phase) after partial hepatectomy. Blots were hybridized with probes specific for the hsp70 (heat-inducible), hsc70 (constitutively expressed), hst70 (testis-specific) and grp78 (glucose-regulated) gene. No hsp70 and hst70 gene transcripts have been detected at any time point investigated, and only a low increase of the hsc70 mRNA level has been observed 24 h after surgery. In contrast, a significant accumulation of the transcript coded by the grp78 gene has been detected in liver remnant 12 and 24 h after partial hepatectomy. However, we observed a comparable activation of this gene in livers of sham-operated rats or in rats injected with turpentine to cause sterile inflammation. Our results indicate that the activation of the grp78 gene in liver of wounded rats (partial hepatectomy or sham operation) is presumably a part of acute-phase response.

Acute-Phase Reaction↗

PubMed1989

[Effects of IPO-63, Carbaryl, Foschlor and Reglone on Newcastle disease virus replication in chick embryo and chick embryo cell cultures].

Cell cultures and chicken embryos were treated with maximum tolerant concentrations of different compounds and infected with Newcastle disease virus simultaneously or 24 hours after the compounds were introduced. The similar results were obtained in both cases. It was found that Reglone inhibited Carbaryl increased virus multiplication. The study on dynamics of virus multiplication indicates that only in the case of IPO and Carbaryl their stimulatory effect on virus at the final stage was preceded by its inhibition.

Animals↗

PubMed1989

[Effect of urea and uric acid on the hemolytic activity of Sendai virus].

It was found that haemolytic activity of Fushimi strain of Sendai virus multiplied in allantoic cavity of chicken embryos is independent on its haemagglutinating titer and also on allantoic fluid urea and uric acid content. It was shown in experiments with embryonated eggs that these two compounds have no also influence on haemolytic activity induction in Sendai virus. Moreover, the results of an experiment in which allantoic fluid was replaced by Eagle's liquid suggest that most probably the other components present in allantoic fluid do not also influence the appearance of haemolytic activity of this virus.

Animals↗

PubMed1989

An evaluation of four serological tests for the detection of antibodies to bovine parainfluenza virus type 3.

Haemagglutination-inhibition (HI), virus neutralization (VN), haemolysis-inhibition (HLI-1, HLI-2) tests, and two new haemofusion-inhibition (HFI-1, HFI-2) tests, developed by us, were tested for the detection of antibodies to bovine parainfluenza virus type 3 (BPIV-3) in sera of 45 steers randomly selected from a herd naturally infected by BPIV-3. Twelve seronegative animals were then vaccinated with formalin-inactivated vaccine and 15 days later tested by the above-mentioned assays. Linear regression analysis revealed positive correlations between HI, VN, HLI-1 and HFI-1 antibody titres, which confirm the specificity of the HFI-1 test. The HLI-1 and HFI-1 assays proved to be less sensitive than the HI and VN ones, while HLI-2 and HFI-2 tests failed to detect any antibody to BPIV-3.

Animals↗

PubMed1988

Isolation of major heat shock protein (hsp70) gene-related sequences from rat genome.

Rat genome was assayed for the presence of hsp70 gene-related sequences. Southern blots prepared from rat DNA digested with EcoRI or HindIII restriction endonucleases were hybridized with mouse, human and fruit fly hsp70 gene probes at increasing stringencies. At the stringency which allows sequences divergent up to about 30% to form stable complexes all three probes detected 25-30 restriction fragments. Increased stringency of the hybridization reduced the number of detectable bands to a few and among them the DNA fragments hybridizing specifically either with mouse or human hsp70 gene probes were detected. Most of the genomic fragments containing hsp70 gene-related sequences were subsequently isolated by screening the rat genomic library with mouse hsp70 gene probe. 168 positive clones were plaque purified and on the basis of the restriction and hybridization pattern we deduced that inserts represented 20 different genomic regions. Partial restriction maps of all isolated genomic fragments were constructed and regions containing hsp70 gene related as well as highly repetitive DNA sequences were localized. A putative sequence rearrangement in the proximity of the hsp70 gene-related sequence was detected in one of the isolated genomic segments.

Animals↗

PubMed1988

Transcriptionally active chromatin can be selectively released by DNase I from Physarum polycephalum genome.

In a simple eukaryote Physarum polycephalum about 13% of the genome is transcribed into abundant cytoplasmic RNA as shown by S1 nuclease digestion of DNA-RNA hybrids. Mild digestion of isolated Physarum nuclei with DNase I liberates a fraction of chromatin 3.5-fold enriched in sequences hybridizing by Physarum poly(A)+ RNA. This fraction is similarly enriched in histone H4 and actin genes known to be actively transcribed in Physarum. High content (about 45%) of actively transcribed sequences in DNase-I-released fraction of Physarum chromatin makes it particularly well suited for studying the structural basis of transcriptional activation in eukaryotes.

Actins↗

PubMed1987

A hsp70-related gene is constitutively highly expressed in testis of rat and mouse.

The expression pattern of major heat shock related genes (hsp70 gene family) in various organs of mouse and rat was investigated using Northern blot analysis. Heat shock gene related transcripts were detected in total RNA by hybridization with cloned mouse hsp70 gene sequences. Cells of various organs of intact mouse and rat constitutively synthesize a 2.2 kb and a 2.5 kb RNA. Exceptionally high levels of the 2.5 kb RNA, 50-250 fold higher than in other tissues are found in testis of both rodents. The 2.5 kb RNA hybridizes strongly to an extended region of mouse hsp70 gene; it also hybridizes poorly to the Drosophila hsp70 gene. The data suggest that the 2.5 kb RNA is transcribed from a hsp70-related gene in mouse and rat.

Animals↗

PubMed1987

Expression of hsp70-related gene in developing and degenerating rat testis.

Rat testes contain highly elevated levels of 2.5 kb RNA transcribed from a heat shock (hsp70) related gene. In the present paper northern blot analysis was used to follow the changes in the 2.5 kb transcript level during the postnatal development of rat testis and during the degeneration of the seminiferous epithelium in adult rats caused by experimental cryptorchidism. The 2.5 kb transcript was undetectable in newborn rats until the 3rd week of life. The level of the transcript reached a maximum at the 4th week and remained unchanged from that point on. Two days after the surgical translocation of the testes from the scrotum into the abdominal cavity the level of 2.5 kb transcript rapidly declined. Presented results strongly suggest that the hsp 70-like gene coding for the 2.5 kb RNA is specifically expressed in germinal cells, most probably in the spermatocytes.

Animals↗