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Biomedical subjects

J Wiśniewski

Publications and source records attributed to J Wiśniewski.

At least 37 records · Page 2Linked to original sources
PubMed1985

A method for isolation of cytoplasmic RNA from a slime mold, Physarum polycephalum.

A procedure for fast and simple preparation of cytoplasmic ribonucleic acid from Physarum polycephalum microplasmodia is described. Microplasmodia are homogenized in a high-magnesium-high-ethylene glycol bis(beta-aminoethyl ether) N,N'-tetraacetic acid buffer and nuclei are pelleted. The supernatant is extracted with sodium dodecyl sulfate-phenol-chloroform and crude RNA is precipitated. This is further purified by selective ethanol precipitation from 6 M guanidinum hydrochloride. This RNA preparation is suitable for direct use in hybridization studies.

Cell Fractionation↗
PubMed1985

Immunoregulating influence of levamisole on migration of leukocytes in vivo and in vitro in patients treated for a long time with corticosteroids.

Migration of peripheral blood leukocytes test in vivo and in vitro was performed in 60 patients with chronic spastic bronchitis. More than half of subjects studied were given Levamisole (Decaris, Richter). On the basis of a three-year-long observation it was shown, that levamisol eliminates a depressive effect of corticosteroids on the migration of leukocytes both in vivo and in vitro.

Adrenal Cortex Hormones↗