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J Winkelmann

Publications and source records attributed to J Winkelmann.

43 records · Page 3Linked to original sources
PubMed1981

Macrophages from different sources, their production of chemiluminescence under various stimuli and the effects of PGE2 and drugs.

We are currently characterizing different macrophages sub-populations according to their release of oxygen free radicals (measured as chemiluminescence) under various stimuli and their responses to drugs and mediators. Mouse macrophages were obtained by peritoneal or lung lavage and stimulated in vitro with opsonized zymosan (OpZ) or the calcium ionophore A23187. Chemiluminescence was measured in a luminometer and responses obtained after 5 min expressed as percentages of standard responses to the stimuli. Almost identical dose-response curves to OpZ were obtained with peritoneal and alveolar macrophages, while the dose-response curve of peritoneal macrophages to A23187 was much shallower. The response to A23187 was generally much weaker than that to OpZ and in both types of macrophages PGE2 was a more effective inhibitor of OpZ-induced chemiluminescence than of that induced by A23187. The response to OpZ in peritoneal macrophages was inhibited in a dose-related manner by both PGE2 and superoxide dismutase, but not by indomethacin, d-penicillamine, piroxicam or phenylbutazone. OpZ but not A23187, is a suitable stimulus for pharmacological studies on macrophage chemiluminescence.

Animals↗
PubMedDate not supplied

[Enzyme, enzyme-histochemical and immunohistological studies in chronic erysipelas polyarthritis of swine].

The chronic Erysipelas-polyarthritis in pigs has been considered an animal model resembling human rheumatoid arthritis. Fifteen specifically pathogenfree (SPF) pigs 45 days old were experimentally infectec with strain T 28 of Erysipelothrix rhusiopathiae-bacteria. During the subsequent 32 weeks several enzymatic, immunohistological and microbiological parameters were monitored. Compared to 5 age and sex matched healthy controls the infected pigs showed increased activity of plasma acid phosphatase starting 4 weeks after the infection. Acid phosphatase activity was usually enhanced in synovial fluid of chronically ill animals. Histochemically increased activity of acid phosphatase, beta-glucuronidase and beta-acetylglucosaminidase was found in lining cells and fibroblasts of the synovial membrane of chronically diseased joints. Immunohistochemically Erysipelas-antigen was demonstrated in the synovial membrane even of those inflamed joints from which no living bacteria had been isolated. The microbiological and immunohistochemical results correlated positively with the enzymehistochemical data. The release of lysosomal enzymes from cells of the synovial membrane in chronically diseased joints due to the influence of Erysipelas-bacteria and the possible implications of persistent bacteria on the perpetuation of chronic Erysipelas-polyarthritis are discussed.

Animals↗
PubMedDate not supplied

[Immunopathologic aspects of chronic erysipelas polyarthritis in swine].

Chronic erysipelas polyarthritis in pigs is considered to be an animal model closely resembling human rheumatoid arthritis. In an experimental study using SPF pigs infected with Erysipelothrix rhusiopathiae several immunopathological aspects were studied for up to 14 months after infection. After an initial febrile phase a progressive polyarthritis and discospondylitis developed at 2 weeks after infection. Quantitative determination of IgG and IgM revealed a significant increase in these immunoglobulins both in the serum and the synovia from arthritic joints. Arthritic joints containing viable Erysipelothrix bacteria at termination of the experiment have a higher content of IgG and IgM than bacteriologically negative joints. During the entire experiment the rheumatoid factor titer, determined by the Waaler Rose test, remained relatively low with increases and decreases. The Erysipelothrix antibody titers are highly significant. The total complement activity in the serum increases discontinuously and shows fluctuations similar to those of the rheumatoid factor titer. The highest enzyme activities of acid phosphatase and lactate dehydrogenase are demonstrable in the synovial fluid of arthritic joints with positive bacteriological or immunohistological findings. These joints also have the greatest numbers of IgG-producing plasma cells. By means of immunohistological methods Erysipelothrix antigen can be demonstrated in macrophages of the proliferating synovial membrane. Finally, there is a discussion of the pathogenic significance for the perpetuation of chronic polyarthritis of Erysipelothrix persisting in arthritic joints in spite of focal immune reactions.

Acid Phosphatase↗