Biomedical subjects

J Zhou

Publications and source records attributed to J Zhou.

At least 343 records · Page 19Linked to original sources

PubMed2000

[Epidemiological feature on diabetes mellitus among permanent inhabitant in the Special Economic Zone of Shenzhen].

OBJECTIVE: To investigate prevalence and relevant factors of diabetes mellitus among permanent inhabitant in Shenzhen. METHODS: The prevalence of 8 200 inhabitants aged over 20 in Shenzhen was studied. Blood glucose was determined by glucose - oxidase method. RESULTS: According to the WHO diagnostic standard, the prevalence rates of DM and IGT were 4.23% and 11.94% respectively. The prevalence increased apparently with age. The history of clan and overweight were related to the prevalence rates of DM and IGT. The people of DM and IGT were mostly accompanied with high levels of triglyceride, total cholesterol, uric acid, creatinine and low level of high dense lipoprotein (HDL). CONCLUSION: The history of clan, old age, overweight and higher fattiness were found risk factors of DM and IGT.

Adult↗

PubMed2000

[Activity of serum paraoxonase and its polymorphism in healthy Chinese of Shanghai].

OBJECTIVE: To study activity of serum paraoxonase and its polymorphic distribution in healthy people of Shanghai and to set a reference value for it. METHODS: Serum activities of paraoxonase, salt-stimulated paraoxonase and arylesterase were measured in 198 subjects (105 males and 93 female, aged 21 - 61 years) in Shanghai. Three phenotypes of serum paraoxonase could be distinguished based on the ratio of salt-stimulated paraoxonase activity and paraoxonase activity and the ratio of salt-stimulated paraoxonase activity and arylesterase. Its gene frequency was calculated according to Hardy-Weinberg equation. RESULTS: Activity of serum paraoxonase was 97.6 - 472.1 unit. There was no difference in serum paraoxonase activity between male and female, nor among different ages. It was found that 27 persons in low, 71 in medium and 100 in high activity. Gene frequency of low activity (PON * A) and high activity (PON * B) was 0.369 and 0.631, respectively. CONCLUSIONS: Distribution of serum paraoxonase in healthy people of Shanghai was different from that in Caucasians.

Adult↗

PubMed2000

[Study on the needle biopsies of 100 prostatic adenocarcinoma].

OBJECTIVE: To study the morphological diagnostic criteria of prostatic adenocarcinoma in needle biopsy. METHODS: 250 cases of prostatic needle biopsy including 100 cases of adenocarcinoma were observed retrospectively. The diagnostic significance of 20 parameters of morphological criteria including the architectural changes, cytologic features, invasion and some additional histologic features (intraepithelial neoplasm, intraluminal crystelloid body, intraluminal basophilic mucin secretion, collagen nodule and coagulative necrosis) were analyzed. RESULTS: All 20 parameters of morphological criteria had practical significance in pathologic diagnosis, the most significant ones being architectural changes, prominent nucleoli, perineural and stromal invasion. CONCLUSION: The diagnosis of prostatic adenocarcinoma is based on a constellation of cytologic and architectural features.

Adenocarcinoma↗

PubMed2000

[The effects of hypertonic saline solution perfusion on the isolated hearts of burned rats].

OBJECTIVE: To observe the effect of hypertonic saline solution perfusion on the function of isolated hearts of burned rats. METHODS: The isolated rat heart was taken as the model. The hearts from normal control and burned rats were perfused by improved isotonic (Na(+) 118 mmol/L) and hypertonic Krebs-Henseleit (Na(+) 215 mmol/L) solutions, respectively. The dynamic changes of the cardiac function were monitored during heart perfusion. RESULTS: The cardiac contractive and diastolic function were transiently inhibited and followed by quick recovery during the perfusion with hypertonic solution in both control and burned rat hearts. But when the hypertonic solution was replaced by isotonic, there appeared significant enhancement of the cardiac contractive and diastolic function. These findings indicated that hypertonic solution exerted more powerful influence on the contractive and diastolic function of burned rat heart. CONCLUSION: Hypertonic saline solution could influence the cardiac contractile and diastolic function significantly. And the magnitude of the effects was correlated with the basic cardiac condition.

Animals↗

PubMed2000

[Seven years' summary report on the treatment of acute promyelocytic leukemia with arsenic trioxide--an analysis of 242 cases].

OBJECTIVE: To observe the effectiveness of arsenic trioxide (As(2)O(3)) in the treatment of acute promyelocytic leukemia (APL). METHODS: Data of clinical observation, seven year follow-up survey were analysed with American SAS system software, concentration of arsenic in cerebrospinal fluid (CSF) was determined by atomic absorbing spectrum. RESULTS: The complete remission (CR) and effectiveness rates were 87.9% and 94.4% for the previously untreated group; 60.0% and 65.0% for the relapsed in As(2)O(3) maintenance treatment group; 69.5% and 84.8% for the relapsed in other than As(2)O(3) maintenance treatment group; and 48.7% and 94.1% for the refractory group, respectively. In the previously untreated group, the relapse rate at 3 years after CR was 26.7%. The probability of survival in 136 follow-up survey patients was 92.0% and 76.7% at 5 years and 7 years, respectively. There were mild side effects. Central nervous system leukemia (CNSL) occurred in 138 out of 412 cases in 6 years, including some refractory leukemia, extramedullary residual leukemia and extramedullary relapse. Prophylactic intrathecal injection could reduce the incidence rate of CNSL. Determination of arsenic concentration indicated that it was difficult for arsenic to penetrate the blood-brain barrier. CONCLUSION: The CR rate and long-term survival are high and the relapse rate is low in APL patients treated with As(2)O(3). There is no cross-resistance between ATRA and As(2)O(3) and other chemotherapeutic agents. As(2)O(3)-resistance occurs scarcely. It can also be used in maintenance treatment.

Adolescent↗

PubMed2000

[Exploration of gene expression profiles of CD(34)(+) hematopoietic stem/progenitor cells based on large scale sequencing].

OBJECTIVE: To set up a large scale sequencing system and explore the gene expression profiles of CD(34)(+) hematopoietic stem/progenitor cells (HSPCs). METHODS: CD(34)(+) cells were isolated from umbilical cord blood and subjected to cDNA library construction. A primary profile of gene expression in HSPCs was emerged by EST sequencing and bioinformatics analyzing. RESULTS: Among 9,866 ESTs thus obtained, 7 476 meaningful ESTs were clustered into 2,060 unique sequence species (USSs), representing 1,054 known gene species and 1,006 unknown gene fragments. The 1054 known genes were divided into 8 categories: (1) hematopoiesis associated: 73, (2) chromatin structure and cell division/apoptosis: 91, (3) signal transduction and receptors: 111, (4) cell structure/mobility: 48, (5) cell/organism defense/homeostasis: 41; (6) Gene expression (transcription, translation and modification): 265, (7) metabolism: 192; and (8) unclassified: 233. CONCLUSION: A gene expression profile including 1,054 known genes and 1006 new gene fragments of HSPCs was primarily obtained, which may lay a basis for the further study on the molecular mechanism of hematopoiesis regulation and provide candidates for new gene cloning.

Antigens, CD34↗

PubMed2000

[The sensitivity to apoptosis is enhanced in U937-ASPI-3K cells which stably express antisense ATM/PI-3K].

OBJECTIVE: To analyse the characteristics of apoptosis sensitivity in U937-ASPI-3K cells which is stably expressing anti-sense ATM/PI-3K. METHOD: Annexin V-PI apoptosis detection kid and flow cytometry were used to determine apoptosis rate, Western blot to the expression level of cytochrome C protein and Bcl-2 protein. RESULTS: U937-ASPI-3K cells had a lowered threshold for triggering apoptosis in response to low dose irradiation. Cell apoptosis rate was 28% and (53.0 +/- 5.4)%, respectively, at 8 and 48 hours after 1.5 Gy (137)Cs irradiation, while in U937-pZEOSV2 (+) control group it was 4.2% and (11.0 +/- 3.3)%, respectively. Cytosolic cytochrome C protein band was gradually intensified in U937-ASPI-3K in 4 - 8 h after irradiation, but was not detected in control group. Bcl-2 protein gradually decreased in U937-ASPI-3K in 4 - 8 h after irradiation, but didn't change obviously in control group. CONCLUSION: Cell line U937-ASPI-3K acquired a phenotype with a lowered threshold for triggering apoptosis. It demonstrated that selective inactivation of ATM gene might be of considerable value in tumor treatment.

Apoptosis↗

PubMed2000

[Experimental study of protective effect of qingkailing on glutamate induced neurotoxic damage of brain].

OBJECTIVE: To observe the effect of Qingkailing (QKL) on glutamate induced damage of brain, in order to seek for effective drugs for antagonizing neurotoxicity of glutamate. METHODS: Number and morphology metrology of neuron in cerebral cortex and hippocampus were detected by using MIAS-300 image analysing instrument, electron microscope and immunohistochemical methods. RESULTS: QKL could allevate the glutamate induced accumulation of water and sodium in the brain tissue, relief the damage of cerebral cells and reduce the percentage of c-fos positive cell in brain. CONCLUSION: QKL could protect brain damage induced by glutamate, which might be related to inhibition of QKL on glutamate caused enhancement of c-fos gene expression.

Animals↗

PubMed2000

[Inhibition of transmembrane K(+) currents in mammalian ventricular myocytes by antiarrhythmic agent RP62719].

The effect of RP62719 on the inward rectifier K(+) current (I(K1)),transient outward K(+) current (I(to)) and delayed outward K(+) current (I(K)) in isolated cardiac myocytes was determined using the whole cell patch clamp technique in guinea pig and dog. RP62719 decreased I(K1) with an inhibitory concentration 50 (IC(50) ) of 5.0+/-1.0 micromol/L at -100 mV in guinea pig ventricular cells. In dog ventricular myocytes, RP62719 inhibited Ito by 84+/-4.4% with an IC(50) of 1.2+/-0.51 micromol/L at +40 mV. In guinea pig ventricular cells, RP62719 decreased I(K): I(Kstep) by 50.0+/-8.3%%and I(Ktail) by 56.0+/-4.9% at +40 mV, respectively. RP62719 inhibited I(Kstep) with an IC(50) of 4.2+/-0.8 micromol/L and I(Ktail) with an IC(50) of 3.3+/-0.75 micromol/L. Thus it is suggested that the ionic mechanism of antiarrhymic effect by RP62719 may be due to its inhibition of I(K1),I(to) and I(K).

Animals↗

PubMed2000

[The relationship of brainstem auditory evoked potential to cerebral blood flow volume in with vertebrobasilar transient ischemic vertigo].

This study was aimed at the relationship of brain-stem auditory evoked potential(BAEP) with cerebral blood flow(CBF) volume and vascular pathological changes in patients with vertebro-basilar transient ischemic vertigo (VBTIV). 65 patients were examined by magnetic resonance angiography(MRA), transcranial Dopplar(TCD) and BAEP; 26 controls were examined by MRA and TCD. In the patient group, MRA showed that vascular pathological changes were obvious in 50 patients, and obscure or absent in 15 patients. The CBF volume [112.3-278.9 ml/min (2s)] of control group was higher than that (48.0-262.0 ml/min) of the patients group (t = 2.43, P < 0.01) in which 15 patients had low CBF volume and 50 patients had normal CBF volume. The BAEP of 47(72.3%) patients was abnormal. Out of 15 patients with low CBF volume, 14(93.3%) had abnormal BAEP, but out of 50 patients with normal CBF volume, only 33(66%) patients had abnormal BAED (chi 2 = 4.34, P < 0.05). In the 50 patients with obscure obvious vascular pathological changes, 40(80%) patients had abnormal BAEP, but in the 15 patients with obscure or without the changes, only 7(43.3%) patients had abnormal BAEP (chi 2 = 4.86, P < 0.05). These results suggested that there might be a close relationship of BAEP with CBF volume and vascular pathological changes.

Adult↗

PubMed2000

[Establishment and application of a computerized radiography cephalometrics system].

We have developed a new-typed computerized radiography cephalometrics and prediction system. It includes cephalometrics, growth prediction, orthopedics/orthodontics prediction and profile prediction. In this study, 47 lateral cephalograms were randomly selected to process a reliability test. The results showed the two groups were in agreement on all of the 25 measurement index. The system is easier, quicker and less prone to error than traditional cephalometrics approach. It is convenient for orthodontists.

Cephalometry↗

PubMed2000

[The study of occlusal contact areas of upper premolars and molars in the unilateral cleft lip and palate].

OBJECTIVE: Occlusal contact plays an important roll in masticatory procedure. It infers to biting force and masticatory performance. In order to analysis the oral function in UCLP, the author tested the occlusal contact areas of upper premolars and molars in UCLP. METHODS: 22 UCLP patients (male 13, female 9, permanent teeth) were selected, which had no temporomandibular joint dysfunction. All of them had received plastic surgery treatment and never received orthodontic therapy. Their occlusal contact areas of upper premolars and molars in intercaspid position were recorded by performed soft wax, and the results were managed by computer with the software of micro image analysis system. All the data were input to computer and be analyzed by statistics. RESULTS: 1. In normal people, the occlusal contact areas of upper premolars and molars had no differences in bilateral and in sexual. 2. there was no sexual difference in UCLP as that in normal group, and the occlusal contact areas in cleft side or non-cleft side had no difference yet. 3. The occlusal contact areas in UCLP were lower than that in normal people. 4. The decrease of occlusal contact areas was the most important factor to low masticatory performance in UCLP patients. CONCLUSION: After plastic surgery in UCLP, orthodontic therapy will improve masticatory contact and contribute to craniofacial esthetic and masticatory function.

Adolescent↗

PubMed2000

[Determinations and analysis of masticatory performance in unilateral cleft lip and palate].

OBJECTIVE: Masticatory function is one of the most important physical functions in craniomaxilla system. Masticatory performance is evaluated with the degree of broken dawn tested food in a limited period of time. Unilateral cleft lip and palate (UCLP) is a congenital abnormality in craniofacial growth and developing. It seriously damages face esthetics and masticatory function in UCLP patients. METHODS: 28 UCLP patients (male 17, female 11, permanent teeth) were selected, which all had received plastic operation treatments and never received any orthodontic therapy. The masticatory performance was determined by the advanced light absorbtion method and took peanuts as tested food. Meanwhile, malocclusal patterns and castings were identified in clinic. All of the data were treated with t-test and step wise-regression in statistics. RESULTS: 1. Masticatory performance of male was higher than that of female in normal people, but there was no sexual difference in UCLP. 2. Masticatory performance in UCLP was lower about 53.97% than that in normal people. 3. Different masticatory times within limited time had no effects on masticatory performance. 4. Different Patterns of malocclusion had different effects on masticatory performance. CONCLUSION: Orthodontic therapy is necessary to improve masticatory function in UCLP after plastic surgery.

Adolescent↗

PubMed2000

[The changes of serum angiotensin converting enzyme activity in Type 2 diabetes and its vascular complications].

This investigation was made with reference to the changes of serum angiotensin converting enzyme (SACE) activity in type 2 diabetes and its vascular complications. SACE activity was studied in 127 type 2 diabetic patients and 90 healthy persons by using a spectrophotometric assay. The results showed SACE activity was obviously higher in diabetic patients (459.51 +/- 175.85 U) than in healthy persons (321.14 +/- 121.27 U); SACE activity was significantly higher in type 2 diabetic patients with diabetic nephropathy (548.27 +/- 166.60 U) than in patients without diabetic nephropathy (383.2 +/- 139.00 U), but there was no difference between patients with microalbuminuria and macroalbuminuria; no statistical difference was detected in SACE activity between diabetic patients with diabetic retinopathy (465.64 +/- 178.93 U) and without retinopathy (449.07 +/- 170.04 U); SACE activity was not associated with the course of diabetes, blood pressure, blood lipid and blood glucose. These data suggest that raised SACE activity might only play a role in the initiation of type 2 diabetes and diabetic nephropathy, but not relate to the progress of diabetic nephropathy, the onset of diabetic retinopathy and hypertension.

Aged↗

PubMed2000

[The establishment of a computerized growth prediction system].

Natural craniofacial growth plays an important role in the etiology of malocclusion. So, craniofacial growth prediction is very necessary in orthodontics. The present author have developed a new-typed computerized craniofacial growth predition system, which was designed in the general sense of craniofacial growth. According to a unique method, the system could automatically predict of craniofacial growth to different craniofacial growth pattern. It can be used as an auxilliary tool in clinical, teaching and researches in orthodontics.

Adolescent↗

PubMed2000

[Cloning and expression of otsA gene in E. coli].

1.5 kb of otsA gene encoding trehalose synthase has been cloned by PCR amplification. The DNA fragment was ligated to multi-copy vector and transformed to otsBA deleted and otsA deficient strains of E. coli separately. The transformants exhibited growth as well as the otsBA+ wild type on medium containing 0.5 mol/L NaCl. Trehalose was synthesized and accumulated in the transformed cells under osmotic pressure, which was determined by thin layer chromatograph. The results confirmed that otsA gene was functionally expressed in the recipient strains. These studies suggested that engineering otsA gene and trehalose accumulation into crop plants may improve drought and salinity tolerance.

Cloning, Molecular↗

PubMed2000

[Study on the improvement of plasmid stability in soybean rhizobia].

By using luxAB as the report genes, 3.2 kb parCBA/DE gene fragment from pTR102 was inserted into pLAFR3 which contained a 3.7 kb enhancing fragment and deleted its cos site. Recombinant plasmids pHN155 and pHN156 were obtained. Contrasted plasmids pHN157 and pHN158 which contained cos site were also constructed. These four plasmids were transferred into Sinorhizobium fredii HN01 by tri-parental mating, and plasmid pHN155 and pHN158 were introduced into Bradyrhizobium japonicum TA11 by two-parental mating. The stability of the above plasmids was compared under free-living conditions and the results showed that the parCBA/DE could obviously enhance plasmid stability both in S. fredii and B. japonicum, and deletion of cos site showed only less effect.

Bradyrhizobium↗

PubMed2000

[Ocular axial length and refractive changes in pediatric pseudophakia].

PURPOSE: To evaluate the ocular axial length and refractive changes that occured in children who had cataract extraction with intraocular lens implantation and to investigate the factors that influenced the pseudophakic refraction. METHODS: A review of 12 eyes in 10 children (mean age 7.16 years, range 3.83 to 10.16 years) who had cataract extraction with intraocular lens implantation was undertaken. Patients were followed for an average of 35.8 months. The preoperative and the last postoperative axial length and corneal curvature (K readings) in both operated and unoperated eyes were measured. The distance from the vertex of the cornea to the anterior vertex of intraocular lens (AVpc) was measured in the final follw-up. The initial (in two weeks) and the last refractive status of pseudophakia were examined. Overall age at surgery averaged 7.16 years (range 3.83 to 10.16 years), with followed up of 35.8 months. RESULTS: In the 12 operated eyes, the mean axial growth was 0.39 mm, whereas in the other eight unoperated eyes it was 0.66 mm. Though the axial length of both eyes increased significantly after surgery (P < 0.05), there was no significant difference in the postoperative increase of axial length between the two groups(P > 0.05). In K readings, there was no significant difference between the operated and unoperated eyes before surgery and in the last follow-up respectively. There was no significant difference between the preoperation and the last follow-up in the operated and the unoperated eyes respectively. The average calculated AVpc (AVpc2) was 4.66 30 mm, but the average objective AVpc (AVpc1) measured with ultrasonic biometry was 3.83 mm. The average difference between them was 0.8320 mm and was significant (P < 0.01). According to SRK formula, using preoperative and the last postoperative axial length and corneal curvature to predict refractive changes, the myopic shift was -1.53 D, but the average objective refractive difference between the last and initial examination after surgery was -3.86 D. The average difference between the predicted and actual postoperative refractive changes was significant (P < 0.05). CONCLUSION: Our study suggests that there may be no effect on ocular growth in children (3 to 10 years) followed cataract extraction and intraocular lens implantation. Increasing of axial length and moving forward of intraocular lens induced by complication may result in myopic shift in pseudophakia of children. Slightly undercorrected eyes with intraocular lens in children after cataract extraction will gradually move to emmetropia or moderate myopia in adulthood.

Adolescent↗