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Jing Zhang

Publications and source records attributed to Jing Zhang.

At least 145 records · Page 8Linked to original sources
PubMed2006

[Expressions and clinical significance of urokinase-type plasminogen activator (uPA) and uPA receptor (uPAR) in tongue squamous cell carcinoma].

PURPOSE: To study the expressions and clinical significance of urokinase-type plasminogen activator (uPA) and uPA receptor (uPAR) in tongue squamous cell carcinoma (TSCC). METHODS: The expressions of uPA and uPAR at protein level were examined in 74 cases of TSCC and 15 cases of normal peripheral tissues around cancer (as control) by strep avidin-biotin complex (SABC) immunohistochemical technique. The relationship between the expressions of uPA and uPAR was evaluated by the Spearman's rank correlation test. Mann-Whitney U-test was used to examine the association of these factors with conventional clinical pathological factors. Pearson's Chi-square test was used to analyze the relationship between uPA, uPAR and cervical lymph node status. RESULTS: The rates of the positive expression in TSCC of uPA and uPAR were 71.6% and 64.8% respectively, and a positive relationship between expression of uPA and uPAR was found (P<0.05). There was significant correlation between the level of uPA expression and TNM stage or cervical lymph node status, either between the level of uPAR expression and cervical lymph node status. Pathological grade was not significantly related to the level of uPA expression. Pathological grade and TNM stage were not significantly related to the level of uPAR expression. TSCC cases with concurrent positive expression of these proteins revealed significant higher tendency of cervical lymph node metastasis than those with concourse negative expression (P<0.05). CONCLUSIONS: The expression of uPA and uPAR increased in TSCC, and uPA, uPAR may contribute significantly to TSCC invasion and metastasis.

Carcinoma, Squamous Cell↗
PubMed2006

[Expression of ETV5 and MMP-7 in early stage cervical squamous cell carcinoma and its role in lymphatic metastasis].

BACKGROUND & OBJECTIVE: Transcription factor Ets variant gene 5 (ETV5) participates in tumor metastasis, and matrix metalloproteinase-7 (MMP-7) also plays an important role in tumor invasion and metastasis. This study was to investigate the expression of ETV5 and MMP-7 in cervical squamous cell carcinoma, and explore the roles of ETV5 and MMP-7 in invasion and metastasis of early stage cervical squamous cell carcinoma. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of ETV5 and MMP-7 mRNA in 86 samples of cervical squamous cell carcinoma and 21 samples of normal cervix, and immunohistochemistry was used to detect the expression of MMP-7 protein in these samples. RESULTS: The mRNA levels of ETV5 and MMP-7 were significantly higher in cervical cancer tissues than in normal cervix tissues (0.507+/-0.073 vs. 0.201+/-0.057, P<0.01; 0.488+/-0.078 vs. 0.305+/-0.064, P<0.01), and were significantly higher in nodal metastasis group than in no nodal metastasis group (0.538+/-0.063 vs. 0.496+/-0.073, P<0.05; 0.542+/-0.066 vs. 0.468+/-0.072, P<0.01). The protein level of MMP-7 was significantly higher in cervical cancer tissues than in normal cervix tissues (3.826+/-1.427 vs. 1.714+/-0.383, P<0.01), and was significantly higher in nodal metastasis group than in no nodal metastasis group (4.935+/-1.614 vs. 2.623+/-1.086, P<0.01). The positive rate of MMP-7 protein was significantly higher in lymph node metastasis group than in no lymph node metastasis group (65.21% vs. 36.51%, P<0.01). ETV5 expression was positively correlated to MMP-7 expression (r=0.221, P<0.05). CONCLUSION: ETV5 and MMP-7 are highly expressed in metastatic cervical squamous cell carcinoma, and may be served as markers in estimating the invasive and metastatic potential of cervical squamous cell carcinoma.

Adult↗
PubMed2006

[Brief discussion on attitude of selecting point and posture of needling].

OBJECTIVE: To promote the study on standardization of attitude of selecting point and posture of nee dling, so as to ensure safety of acupuncture and increase clinical therapeutic effect. METHODS: Study, analyze and clinically test and verify on literature of past dynasties, and differentiate and analyze differences and significances of attitude of selecting point and posture of needling, indicate the reasonable parts of ancient and modern various theories, and relative contents should be purified, corrected in time, and put forward a proposal on the standardization. CONCLUSION: Attitude of selecting point and posture of needling have close relation and have a certain difference; the experience of predecessors should be explored and systematized, and present confusion and out-of-date contents should be purified and relative standards should be established as early as possibly.

Acupuncture↗
PubMed2006

Effects of human connective tissue growth factor gene transfection on migration of human umbilical vein endothelial cell.

Connective tissue growth factor (CTGF) is a 38-kDa cysteine-rich protein and an important regulator of angiogenesis. In order to study the role CTGF gene playing in angiogenesis, the eukaryotic expression vector of CTGF gene was constructed in this study, and the role of endogenous CTGF on migration of human umbilical vein endothelial cell (HUVECs) was investigated. According to human CTGF cDNA sequence, a pair of specific primers containing digestion sites of Xba I and Hind III on the 5' end respectively were designed. Reverse transcript polymerase chain reaction (RT-PCR) was used to amplify CTGF cDNA from HUVECs. The eukaryotic expression vector pcDNA3.1(-)/CTGF containing the entire coding region was constructed successfully. Compared with CTGF sequence of GenBank, DNA sequence analysis showed that this reformed plasmid contained the same full length of CTGF cDNA. The results of Western blotting demonstrated that CTGF was over-expressed at 48 h after transfection. Migration of HUVECs transfected with CTGF vector increased significantly compared with those transfected with vector control. In conclusion, the eukaryotic expression vector pcDNA3.1(-)/CTGF was constructed successfully and the endogenous CTGF promoted the migration of HUVECs. This study lays a foundation for further study on the role CTGF gene playing in angiogenesis.

Cell Movement↗
PubMed2006

Down-regulation of hepatocyte growth factor mRNA in rat cardiac myocytes under hypoxia mimicked by cobalt chloride.

Hypoxia is generally considered to represent a fundamental stimulus for angiogenesis. Most angiogenic factors were up-regulated by hypoxia, but as a strong angiogenic and antiapoptotic factor, hepatocyte growth factor (HGF) was down-regulated by hypoxia. In order to investigate whether hypoxia inducible factor 1a (HIF-1alpha) participate in the transcriptional regulation of HGF under hypoxia, rat cardiac myocytes were treated with cobalt chloride (CoCl2), the specific inducer of HIF-1alpha, for different times, and total RNA and protein were isolated to perform RT-PCR and Western blot. Results show the expression of HGF mRNA in cardiac myocytes decreased distinctively after treating with CoCl2 for 12 hours. However, at the same time, the expression of HIF-1alpha protein was up-regulated. HIF-1alpha may be participate in the transcriptional regulation of HGF indirectly.

Animals↗
PubMed2006

[Study on immunorepressive mice model by gamma irradiation].

OBJECTIVE: To establish the immunorepressive mice model by irradiation. METHODS: The 90 Kunming mice which the weight is from 30 to 34g were treated with 3Gy, 4Gy, 5Gy gamma irradiation, the delayed allergy testing, the serum haemolysin level testing, the phagocytosing functions testing and the NK cell activity testing were performed at 3th, 7th, 14th, 21th days after irradiation respectively. RESULTS AND CONCLUSION: (1) All 3Gy, 4Gy, 5Gy gamma irradiation can cause mice immunorepressivestate except the NK cell activity. (2) The time of comeback after irradiation is different from immunity items. The serum haemolysin level is most sensitivity in all, but it comeback most slowly after irradiation. The phagocytosing functions comeback most rapidly after irradiation. (3) It suggests that the irradiation immunorepressive mice model can be established using it in order to evaluate the enhanceing immune function, assisting irradiation hazard protection function and alleviating toxicity function of irradiation treatment and chemical treatment. (4) The NK cell activity is not suitably to immunorespressive by gamma irradiation.

Animals↗
PubMed2006

[Mechanisms of inhibiting proliferation and inducing apoptosis of human gastric cancer cell line SGC7901 by ursolic acid].

BACKGROUND & OBJECTIVE: Some studies have showed that ursolic acid (UA) can inhibit proliferation and induce apoptosis of many tumor cell lines, however its effect on gastric cancer cells has rarely been reported. Cyclooxygenase-2 (COX-2) is highly expressed in various precursor lesions and cancerous tissues. This study was to investigate the effect of UA on COX-2, Bcl-2 and Bax expression in human gastric cancer cell line SGC7901, and explore its potential mechanisms of inhibiting proliferation and inducing apoptosis. METHODS: MTT assay was used to observe the effect of UA (0, 10, 20, 30, 40 micromol/L) on proliferation of SGC7901 cells. Cell apoptosis was observed by fluorescence microscopy when treated with UA for 24 h. Cell cycle and apoptosis were analyzed by flow cytometry (FCM). The expression of COX-2, Bcl-2 and Bax was detected by Western blot. The level of prostaglandin E2 (PGE2) was measured by radioimmunoassay (RIA). RESULTS: UA (20-40 micromol/L) significantly inhibited the proliferation of SGC7901 cells in dose-and time-dependent manners, the IC50 value of UA at 12 h, 24 h, 36 h, 48 h were (57.50+/-1.18) micromol/L, (34.28+/-2.05) micromol/L, (27.54+/-1.11) micromol/L, and (24.83+/-1.02) micromol/L, respectively. When treated with 20-40 micromol/L UA for 24 h, SGC7901 cells were arrested at G0/G1 phase, and the apoptosis rates were (9.10+/-2.39)%, (26.30+/-1.25)%, and (35.20+/-2.26)%, respectively; meanwhile, COX-2 expression and its catalysate PGE2 were decreased, Bcl-2 expression was also decreased, whereas Bax expression was unchanged. CONCLUSION: UA could inhibit proliferation and induce apoptosis of SGC7901 cells through arresting cell cycle, inhibiting COX-2 expression to reduce PGE2 production and down-regulate Bcl-2 expression.

Antineoplastic Agents, Phytogenic↗
PubMed2006

[Acute hemodynamic effects of intravenous recombinant human brain natriuretic peptide in patients with acute myocardial infarction complicated with heart failure].

OBJECTIVE: To compare the acute hemodynamic effects and safety of intravenous injection of recombinant human brain natriuretic peptide (rhBNP) versus intravenous nitroglycerin (NIT) in acute myocardial infarction (AMI) patients with heart failure. METHODS: On top of standard therapy, 42 consecutive patients who suffered from anterior wall AMI with heart failure [pulmonary capillary wedge pressure (PCWP) > 16 mm Hg] within 12 to 24 hours from the onset of chest pain were randomized into rhBNP group (n = 21, 1.5 microg/kg bolus intravenous injection followed by 0.0075 microg.kg(-1).mn(-1) for the first 3 hours and 0.015-0.03 microg.kg(-1).mn(-1) infusion for following 21 hours) and NIT group (n = 21, 10 to 100 microg/mn intravenous infusion for 24 hours). The hemodynamic parameters were monitored by Swan-Ganz catheter at baseline, during drug infusion and 6 hours post infusion withdraw; total urine output was also obtained. The major adverse cardiac events (MACE) were observed up to 1 week after drug infusions. RESULTS: Central venous pressure and systolic blood pressure remained unchanged after rhBNP or NIT infusion. Compared to baseline level, PCWP was significantly reduced by 48.9% (P < 0.01) at 30 minutes after rhBNP infusion and this effect remained up to 6 hours post infusion withdraw; PCWP reduced by 28.7% (P < 0.05) at 2 hours after NIT infusion and this effect remained to 6 hours before infusion withdraw. Cardiac index (CI) was increased by 27.1% (P < 0.05) at 1 hour after rhBNP infusion and remained till 6 hours post infusion withdraw; CI was significantly increased at 3 hour after NIT infusion and this effect disappeared after infusion withdraw. The PCWP and CI values were significantly higher in rhBNP group than that of NIT group at 30 minutes and 2 hours (P < 0.05). Heart rate was significantly reduced at 30 minutes (95.3 +/- 7.4 vs. 118.0 +/- 8.2 bpm, P < 0.05) and at 2 hour (92.8 +/- 6.8 vs. 109.2 +/- 7.6 bpm, P < 0.05) in rhBNP and NIT group, respectively and heart rate remained reduced during the whole infusion period in both groups. The total urine output for 30 hours in rhBNP group (1870 +/- 535 ml) tended to be higher than that in NIT group (1538 +/- 620 ml, P > 0.05). There was no symptomatic hypotension or other adverse events during drug infusion in both groups and MACE up to 1 week post drug infusion was also similar between the two groups. CONCLUSION: Intravenous injection of rhBNP results in more rapid and long-lasting hemodynamic improvements than that of NIT in AMI patients with heart failure and it is also feasible and safe for clinic use in AMI patients with heart failure.

Aged↗
PubMed2006

[Expression of NF-kappaB/IkappaB signal pathway in renal tissues of hepatitis B virus-associated nephritis].

AIM: To investigate the expression of nuclear transcription factor-kappa B (NF-kappaB)/IkappaB in renal tissues of hepatitis B virus-associated nephritis (HBVGN) and to study its role in the pathogenesis of HBVGN. METHODS: The expression of NF-kappaB p65 and IkappaB-alpha in renal tissues was examined in biopsy specimens from 42 HBVGN patients, 20 patients with primary membranous nephropathy and 5 normal subjects by immunohistochemical staining. RESULTS: The NF-kappaB p65 expression in glomerular and tubular of HBVGN tissues was notably higher than that in normal control tissues (P<0.05) and in primary membranous nephropathy tissues (P<0.01). The IkappaB-alpha expression in glomerular and tubular of HBVGN tissues was lower than that in primary membranous nephropathy tissues (P<0.05). CONCLUSION: There might be protein degradation of IkappaB-alpha and nuclear translocation of NF-kappaB in renal tissues of HBVGN, which suggests that NF-kappaB/IkappaB signal pathway may play an important role in the pathogenesis of HBVGN.

Adolescent↗
PubMed2006

[Steroids isolated from fruit of Hovenia acerba].

OBJECTIVE: To study on the chemical constituents of Hovenia acerba. METHODS: Compounds were isolated with stratography and solvent, and identified by spectroscopy. RESULT: beta-sitoseterol, 3 beta-hydroxy-3-deoxymoronic acid and beta-sitoseteryl-3-O-beta-D-glucopyranoside were obtained from fruit of Hovenia acerba. CONCLUSION: The three compounds were steroids isolated from Hovenia acerba Thunb. for the frist time.

Fruit↗
PubMed2006

[Deletion of OPCML gene and promoter methylation in ovarian epithelial carcinoma].

OBJECTIVE: To investigate the expression of OPCML gene in ovarian epithelial carcinoma and determine the relationship between mRNA expression and methylation of their promoters. METHOD: Twenty normal ovarian tissues and 89 ovarian epithelial tumor specimens (72 malignant, 17 benign), as well as 3 ovarian carcinoma cell lines (SKOV-3, CAOV3, and 3AO), were collected for detection of OPCML gene expression by reverse transcription-polymerase chain reaction and for detection of promoter methylation by restriction enzyme cut analysis from 7. 1999 to 7. 2003. RESULTS: Among ovarian epithelial carcinoma 19.4% expressed OPCML mRNA, while 85% of normal ovarian tissue and 76.5% of benign ovarian tumor. The ratio of expression of OPCML mRNA in ovarian epithelial carcinoma was significantly lower than those of normal (chi2 = 30.108, P = 0.0000) and benign tumors (chi2 = 21.162, P = 0.000). No OPCML mRNA expression was found in SKOV-3 and CAOV3, but was found in 3AO. Methylations were detected in 44.4% of cancer cells promoter, while 0% in normal ovarian tissue and benign ovarian tumors. The ratio of methylation of ovarian epithelial carcinoma was significantly higher than those of normal (chi2 = 13.630, P = 0.0000) and benign tumors (chi2 = 11.797, P = 0.000). Methylation was found in SKOV-3 and CAOV3, but not in 3AO. The relationship between gene expression and promoter methylation was correlated (r = 11.589, P = 0.002), especially at Hap I1 site (r = 11.640, P = 0.004). Methylation was also found in SKOV-3 and CAOV3 cell lines, but not in 3AO cell line. CONCLUSION: Deletion of OPCML gene exists in ovarian epithelial carcinoma cell. The gene promoter methylations, especially Hap II motif, may be one of pathways that contribute the inhibition of OPCML expression.

Adult↗
PubMed2006

[Karyotypes of five species in the Drosophila melanogaster species group].

Based on the traditional knocking-slides and Giemsa-staining methods,this paper firstly analyzed the metaphase chromosomes of five species (D. constricta, D. ohnishii, D. ogumai, D. pseudobaimaii and D. tani) of Drosophila melanogaster species group. The results show that all of the five species share the same chromosomal numbers (2n=8) but with different typical shapes. D. pseudobaimaii and D. tani share the shape 2V, 1R, 1D; D. constricta shows 2V,1R,1D with unclear recognized dot chromosomes; D. ohnishii and D. ogumai share the same chromosomal shapes 2V2R. And that between karyotype and phylogenetic relationships are found to be correspond.

Animals↗
PubMed2006

[A new purification methods of small DNAs--the purification methods with silica wool].

The principal purpose of this study is to set up efficient purification techniques of small DNAs which are suitable for isolation of from tens to three hundred bases of genes. On the bases of the technique, purification methods for big DNA fragments are established. In the experiment, the DNA bands were cut after agarose gel electrophoresis and put into 0.5 mL of tubes with silica wool, glass wood, absorbent cotton and cotton at the bottom. And then 10 000 r/min for 2 min, the liquid was collected. The results indicated that silica wool was the best of the materials. The recovery rate for DNAs below 200bp was over 90%, 85% to approximately 90% for 300bp. And the technique can be applied to purify bigger DNA fragments. The kits for DNA purification hardly recovered DNA below 150bp. The recovery rate for 150bp of DNA was 5%, 60% even for 300bp. The efficiencies of enzymic digestion and enzymic connection for the DNAs purified by the technique were the same as those for the DNAs isolated by the kits. So, the technique is obviously superior to kit purification methods.

DNA↗
PubMed2006

[The clinical manifestations and assessment of post stroke dysphagia].

OBJECTIVE: To find the relationship between the clinical manifestations of dysphagia resulting from stroke and dysfunction of swallowing organs. It is helpful to understand the mechanism of dysphagia so as to guide its diagnosis and therapy. METHODS: Oral facial examination experimental swallowing methods were used to identify the abnormal manifestations of dysphagia. Spearman correlation analysis was used as statistic method. Sensitivity (SN), specificity (SP), predictive values (PV+/-) were calculated. RESULTS: There were about 20 or so abnormal manifestations after stroke and swallowing organs dysfunction include abnormal lip closure, decreased tongue motility, weakness of palate, decreased or disappeared gag reflex, abnormal lift of larynx and insufficient opening of cricopharyngeal muscle. The sensitivity and negative predictive values were high in all the dysfunction organs with the highest sensitivity, specificity, positive and negative predictive value in abnormal lip closure (84.62%), decreased tongue motility (67.75%), insufficient opening of cricopharyngeal muscle (80.96%) and abnormal lip closure (90.91%). CONCLUSIONS: It is comparatively accurate for clinical evaluation to judge dysfunction in lip closure, lingual movement and larynx elevation.

Adult↗
PubMed2006

[Effects of electroacupuncture on GDNF positive cell immunoreactivity in local dermal tissue of the inflammatory pain focus in the rat of adjuvant arthritis].

OBJECTIVE: To study on the analgesic mechanism of electroacupuncture (EA) in the rat of adjuvant arthritis (AA). METHODS: Forty-eight SD rats were randomly divided into a blank control group (n = 8), an inflammatory group (n = 10), an acupoint EA group (n = 10), a non-acupoint EA group (n = 10) and a contralateral acupoint EA group (n = 10). Complete Freund's adjuvant (CFA) 50 microL were injected into the left malleolus' articular cavity in the rats except the blank control group for preparing single local adjuvant arthritis (AA) model. EA was given every other day to the acupoint EA group, the non-acupoint EA group and the contralateral acupoint EA group. The improving effects of EA at "Huantiao" (GB 30) and "Yanglingquan" (GB 34) on the dorsal flexion pain score and the swell of dorsum of hind paw were investigated, and effects of EA on Glial cell line-derived neurotrophic factor (GDNF) positive cells immunoreactivity in the inflammatory tissue of the AA rats on the 14th day after injection of adjuvant were observed with immunohistochemical technique. RESULTS: Hyperalgesia and local swell, and GDNF in the dermal and subcutaneous tissue around the inflammatory ankle joint in the inflammatory groups were significantly higher than those in the blank control group. EA on the ipsilateral and contralateral acupoints reduced the pain, promoted the recovery of swell, and decreased the positive area percentage and mean optical density of GDNF positive cells in the dermal and the subcutaneous tissues. However, the non-acupoint EA group did not have this action. CONCLUSION: EA can regulate expression of GDNF in local dermal tissue of the inflammatory focus in the AA rat, so as to exert the anti-inflammatory and analgesic effects.

Animals↗
PubMed2006

[The measurement of the ratio of primary hydroxyl group to second hydroxyl group in polyether polyol by near infrared spectrum].

The method of near infrared spectrum, combined with derivative spectrum and multiple linear regression was applied to measure the ratio of primary hydroxyl group to second hydroxyl group in polyether polyol. The authors obtained good predictive results by using the method in this experiment. The work is very helpful for the on-line measurement of polyether polyol.

English Abstract↗
PubMed2006

[Study on the characterizations of beta-cyclodextrin-benzoylferrocene-thiosemicarbazone inclusion complex and micro-environmental effects].

Inclusion complex of benzoylferrocene-thiosemicarbazone (TBF) with beta-cyclodextrin (beta-CD) was prepared by using kneading method. Continuous variation plot proved that 2:3 (TBF: beta-CD) inclusion complex has been formed. The characterizations of the inclusion complex were studied by UV, FTIR and X-ray diffractometry. By using the data of UV spectroscopy, the binding constant of the inclusion compound was estimated to be 5.04 x 10(13) (L4 x (mol)(-4)), and its change in absorption with time was studied. The stability of inclusion is obviously greater than the guest molecule. Furthermore, the influences of different solvents and pH on the UV spectra of TBF and beta-CD-TBF were discussed respectively. The results show that different micro-environments have apparently different effects on electric spectra of guest and inclusion complexes.

English Abstract↗