Biomedical subjects

Jorge Perdigão

Publications and source records attributed to Jorge Perdigão.

9 recordsLinked to original sources

PubMed2003

Immunocytochemical identification of type I collagen in acid-etched dentin.

The hypothesis tested in this study is that the application of phosphoric acid prevents collagen fibrils (CF) from maintaining their structural morphology, as assessed by their immunochemical antigenicity. Dentin was conditioned with EDTA and with 35% H3PO4 for 15, 30, and 60 s. For a control there was no treatment. A pre-embedding immunohistochemical procedure was evaluated under high-resolution SEM and a postembedding immunolocalization technique was performed on ultrathin sections at TEM level. Conditioning with EDTA resulted in a weaker labeling signal than for dentin conditioned with H3PO4. The most intense labeling was observed after dentin had been etched with H3PO4 for 15 s. The least intensive labeling was evident when the acid was applied for 30 s or for 60 s. A very weak signal was detected on the untreated dentin surface. These results provide evidence that a 15-s application of 35% H3PO4 causes mineral dissolution of the crystals enveloping the superficial CF without damaging the ultrastructure of the CF while longer applications of 35% H3PO4 cause alterations in the CF that decrease their antigenicity.

Acids↗

PubMed2003

Bonding characteristics of self-etching adhesives to intact versus prepared enamel.

PURPOSE: This study tested the null hypothesis that the preparation of the enamel surface would not affect the enamel microtensile bond strengths of self-etching adhesive materials. MATERIALS AND METHODS: Ten bovine incisors were trimmed with a diamond saw to obtain a squared enamel surface with an area of 8 x 8 mm. The specimens were randomly assigned to five adhesives: (1) ABF (Kuraray), an experimental two-bottle self-etching adhesive; (2) Clearfil SE Bond (Kuraray), a two-bottle self-etching adhesive; (3) One-Up Bond F (Tokuyama), an all-in-one adhesive; (4) Prompt L-Pop (3M ESPE), an all-in-one adhesive; and (5) Single Bond (3M ESPE), a two-bottle total-etch adhesive used as positive control. For each specimen, one half was roughened with a diamond bur for 5 seconds under water spray, whereas the other half was left unprepared. The adhesives were applied as per manufacturers' directions. A universal hybrid composite resin (Filtek Z250, 3M ESPE) was inserted in three layers of 1.5 mm each and light-cured. Specimens were sectioned in X and Y directions to obtain bonded sticks with a cross-sectional area of 0.8 +/- 0.2 mm2. Sticks were tested in tension in an Instron at a cross-speed of 1 mm per minute. Statistical analysis was carried out with two-way analysis of variance and Duncan's test at p < .05. Ten extra specimens were processed for observation under a field-emission scanning electron microscope. RESULTS: Single Bond, the total-etch adhesive, resulted in statistically higher microtensile bond strength than any of the other adhesives regardless of the enamel preparation (unprepared = 31.5 MPa; prepared = 34.9 MPa, not statistically different at p < .05). All the self-etching adhesives resulted in higher microtensile bond strength when enamel was roughened than when enamel was left unprepared. However, for ABF and for Clearfil SE Bond this difference was not statistically significant at p > .05. When applied to ground enamel, mean bond strengths of Prompt L-Pop were not statistically different from those of Clearfil SE Bond and ABF. One-Up Bond F did not bond to unprepared enamel. Commercial self-etching adhesives performed better on prepared enamel than on unprepared enamel. The field-emission scanning electron microscope revealed a deep interprismatic etching pattern for the total-etch adhesive, whereas the self-etching systems resulted in an etching pattern ranging from absent to moderate.

Acid Etching, Dental↗

PubMed2002

Dentin bonding as a function of dentin structure.

The traditional principles of operative dentistry have been challenged since Dr. Buonocore introduced the aid-etch technique in 1955. In spite of the numerous changes in clinical protocols and adhesive techniques, adhesion to dentin remains difficult. The importance of micromechanical bonding to dentin has been recognized over the last decade. Researchers now believe that dentin adhesion relies primarily on the penetration of adhesive monomers into the filigree of collagen fibers left exposed by acid etching. Two main strategies are currently in use for bonding to enamel and dentin: the self-etch technique and the total-etch technique. The efficiency of either bonding strategy depends very much on the dentin substrate used for bonding. Laboratory tests use ideal dentin from extracted teeth, which does not reflect the clinical reality. Clinical studies are the ultimate test for any dental adhesive material.

Clinical Trials as Topic↗

PubMed2002

The use of flowable composites as filled adhesives.

OBJECTIVE: The effect of filled adhesives on bonding resin composites to dentin has not been fully understood. Due to their filler content, filled adhesives may act as stress breakers. The aim of this in vitro study was to evaluate the use of flowable composites of different viscosities on bonding to enamel and dentin without the use of an intermediate bonding resin. MATERIALS AND METHODS: Enamel and dentin bond strengths of OptiBond FL, Syntac Classic, and EBS Multi combined either with their proprietary bonding agent or a flowable resin composite (Ultraseal XT Plus or Revolution) were measured. The tests were carried out with a microtensile device at a crosshead speed of 1mm/min after 24h of storage at 37 degrees C in water. Mean bond strengths were analyzed using the Wilcoxon test and multiple comparisons according to the Mann-Whitney U-test. The micro-morphology of corresponding resin-dentin interfaces of the same teeth were analyzed using SEM and TEM. RESULTS: The control groups with adhesive systems used as per manufacturers' protocol showed bond strengths of 38.9-41.1 MPa to enamel and 28.8-33.4 MPa to dentin. With respect to bond strength to etched enamel, only Ultraseal XT Plus as bonding resin reached the level of the control groups. When used as bonding agents on dentin, both flowable composites produced lower microtensile bond strengths to etched and primed dentin than did the control groups. Micro-morphological analysis using SEM and TEM resulted in hybrid layer formation for both control and experimental groups. However, many areas of the resin-dentin interface showed insufficient penetration of the flowable composites at the top of the hybrid layer as well as numerous tubules obstructed by filler particles. CONCLUSIONS: The flowable composites tested in this study should not be used to replace bonding agents. Flowable composites of thinner viscosity, such as Ultraseal XT Plus, may bond to enamel adequately without the requirement of an intermediate bonding resin.

Acid Etching, Dental↗

PubMed2002

In vivo influence of residual moisture on microtensile bond strengths of one-bottle adhesives.

PURPOSE: This study evaluated the microtensile bond strengths of three dentin adhesives applied on clinically moist dentin or on dentin that was dried with air for 5 seconds. The null hypothesis to test was that the level of residual moisture does not influence bond strengths when restorations are placed in vivo. MATERIALS AND METHODS: Twenty-four premolars scheduled to be extracted for orthodontic reasons from patients between the ages of 15 and 23 years were restored with one of the following adhesive systems followed by a mini hybrid composite resin: Excite (Ivoclar/Vivadent), an ethanol-based dentin adhesive; Prime & Bond NT (Dentsply/Caulk), an acetone-based dentin adhesive; and Single Bond (3M ESPE), an ethanol and water-based dentin adhesive. After extraction, the specimens were sectioned with a slow-speed diamond saw in two perpendicular directions to obtain sticks with a cross-section of 0.7 +/- 0.2 mm2. The specimens were attached to a Geraldeli device and fractured using a universal testing machine at a crosshead speed of 1 mm per minute. RESULTS: For each dentin adhesive, there were no statistical differences between means for dry dentin versus moist dentin. Single Bond and Prime & Bond NT ranked in the same statistical subset regardless of the moisture condition of the substrate. Both Excite, dry, and Excite, moist, resulted in statistically lower bond strengths than Single Bond, moist, but similar to those of Single Bond, dry, Prime & Bond NT, moist, and Prime & Bond NT, dry. CLINICAL SIGNIFICANCE: In this study, the level of residual moisture did not influence microtensile bond strengths. Clinically, the degree of moisture left on the dentin surface upon rinsing off the etching gel may not be as relevant as previously reported in laboratory studies.

Acetone↗

PubMed2002

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Dental Materials↗

PubMed2002

Microleakage of Class V composites using different placement and curing techniques: an in vitro study.

PURPOSE: To evaluate microleakage at enamel and dentin margins of two composite resins, using bulk and incremental placement techniques, "rebonding", and facial and lingual curing methods. MATERIALS AND METHODS: One hundred standardized Class V cavity preparations were made on the facial surface of extracted human premolars and were randomly assigned to 10 groups. Single Bond was used as the dentin/enamel adhesive. A heavily filled composite resin, Z250, and a microfill, Silux Plus, were inserted and polymerized using five different techniques: (1) incremental placement and facial curing; (2) incremental placement, facial curing and rebonding; (3) bulk placement and facial curing; (4) bulk placement, facial curing and rebonding; (5) incremental placement and lingual and facial curing. After the restorations were finished and polished, the margins of those in the rebonded groups were etched, rinsed, and dried. The adhesive resin, Single Bond, was applied at the composite resin-tooth interface and light-cured. All the specimens were thermocycled, stained with 1% methylene blue, sectioned, and evaluated for leakage (0-4 scale) by two examiners. RESULTS: Almost no leakage occurred at enamel margins. At the cementum margins, differences in microleakage related to restorative material or technique were not statistically significant. However, leakage at the cementum margins was significantly greater than at the enamel margins for both composite resin materials.

Bicuspid↗

PubMed2002

Influence of NaOCl deproteinization on shear bond strength in function of dentin depth.

PURPOSE: To determine the influence of NaOCl application on shear bond strengths (SBS) to superficial and deep dentin, using Prime & Bond 2.1. MATERIALS AND METHODS: Superficial and deep dentin was exposed in 40 extracted third molars by sectioning the occlusal surface immediately under the enamel-dentin junction or close to the pulp chamber. After polishing the dentin disks (600-grit SiC), they were assigned to two groups: (1) 36% orthophosphoric acid for 15 seconds, or (2): 36% orthophosphoric acid for 15 seconds followed by 5% sodium hypochlorite (NaOCl) for 2 minutes. The dentin adhesive was applied as per manufacturer's instructions, followed by TPH resin-based composite. The specimens were stored in water for 24 hours at 37 degrees C and thermocycled x500 and SBS were determined. Data were statistically analyzed with ANOVA and Student's t-test. RESULTS: For acid-etched specimens, superficial dentin resulted in statistically higher mean SBS than deep dentin. After NaOCl application, deep and superficial dentin resulted in statistically similar mean SBS. Collagen removal did not affect mean SBS on superficial dentin, while SBS values on deep dentin were increased.

Acetone↗

PubMedDate not supplied

Use of a tooth-colored post for anterior restorations.

The restoration of endodontically treated teeth is still a challenge to the general dentist. Extensive caries or traumatic fracture may result in insufficient coronal tooth structure remaining to support a core. The introduction of fiber post systems in the 1990s has provided dentists with new options for the restoration of the pulpless tooth. This clinical article demonstrates the use of a fiber post and a composite buildup material.

Adult↗