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Biomedical subjects

K Francois

Publications and source records attributed to K Francois.

6 recordsLinked to original sources
PubMed2003

Modelling the individual cell lag phase. Isolating single cells: protocol development.

AIMS: To develop a protocol to isolate single cells in wells of a microtitre plate, having a high certainty of individual cells, combined with a sufficient yield. METHODS AND RESULTS: Single cells were obtained using 1/2 dilution series in microtitre plates. Seventy-two Lactococcus lactis dilution series were checked by plate counting. When the last five columns of the plates were observed, the chance of having one single cell was 80%, while the yield was 75 wells containing cells. A simulation model confirmed these results. This method was compared with the commonly applied method. CONCLUSIONS: This method makes it possible to combine a higher chance of having one cell in a microtitre well with a slightly higher yield. SIGNIFICANCE AND IMPACT OF THE STUDY: A tool is developed to isolate single cells to provide a suitable base for investigating and modelling the individual cell lag phase.

Cell Division↗
PubMed2001

Outcomes of quintuplet pregnancies.

OBJECTIVE: To review the obstetric and perinatal complications associated with quintuplet pregnancies in the United States. STUDY DESIGN: The databases of two large support groups for higher order multiple gestations, the Triplet Connection and Mothers of Super Twins, were accessed for quintuplet pregnancies. Each record contained information regarding maternal demographics, obstetric complications and perinatal outcomes. The data were analyzed by the Student t test and chi 2 test as indicated, with a P value < .05 for significance. RESULTS: Complete data were obtained for 36 quintuplet pregnancies. Preterm labor complicated all pregnancies. Six pregnancies delivered prior to 24 weeks' gestation, with no surviving infants. In the remaining 30 pregnancies, the mean gestational age at delivery was 28.9 weeks (SD +/- 2.0). There were trends toward later gestational ages at delivery in pregnancies with prophylactic cerclage placement and among multiparous women; however, these trends were not statistically significant. The perinatal mortality rate for the entire sample was 253/1,000. When adjusted for pregnancies delivered beyond 24 weeks' gestation, the perinatal mortality rate was 83/1,000. Minor and major neonatal morbidity affected 20% (26/132) and 10% (13/132) of quintuplet neonates, respectively. CONCLUSION: Quintuplet pregnancies are associated with high rates of obstetric complications and significant perinatal morbidity and mortality.

Adult↗
PubMed1993

Anomalous origin of the left coronary artery from the pulmonary artery.

A 20-year-old woman underwent successful operative correction of anomalous origin of the left coronary artery from the pulmonary artery by left internal mammary artery bypass graft. The clinical, hemodynamic, and angiographic features of this unusual case are presented. Different therapeutic options are discussed.

Adult↗
PubMed1989

[Treatment of aortic coarctation].

244 cases of coarctation of the aorta were treated surgically from 1953 to 1988. The age of the patients ranged from 3 days to 59 years with an average of 11.2 years. The average age of the patients decreased progressively over the years. 22% of the patients (54 patients) were less than one year old at the time of the operation. The percentage of infants presenting with decompensation and associated cardiac malformation increased progressively over the years. A total of 40 out of 54 patients belong to this group. The surgical procedure--resection and shunt--was a standard one in 89% of all cases. A prosthesis was required in 4.5% of all cases, and a widening patch in another 5.3%. An unusual technique had to be used in 1.7% of cases. The surgical mortality was of 12 patients (4.9%), all belonging to the infant group (3 days to 13 months old). No mortality was observed among older patients, nor among the elective surgical cases. 9 patients were treated for recurrence of coarctation. No case of paraplegia was noted in this series.

Adolescent↗
PubMed1985

Polydioxanone suture material in growing vascular anastomoses. Experimental study.

We studied the growth of vascular anastomoses after use of absorbable suture material in 21 piglets. In six piglets, end-to-end anastomosis of the infrarenal aorta was performed with 5-0 continuous polypropylene suture; stenosis developed in two of these animals. In 15 piglets, continuous polydioxanone sutures were used for the anastomoses, and no stenosis developed. On the contrary, 14 of these anastomoses showed some degree of dilation at the anastomotic site. Burst testing to 300 mm Hg caused no disruption. On histologic examination, only scar tissue at media and intima with good degree of differentiation was noted. Growth of a vascular anastomosis after use of absorbable polydioxanone suture material seems to be perfectly possible without stricture formation. Because the growing process takes years instead of months in human beings, with obviously less stress at the anastomotic site, it is likely that dilatation at the level of the suture line will not occur in clinical use.

Animals↗