Modelling the individual cell lag phase. Isolating single cells: protocol development.

K Francois; F Devlieghere; A R Standaert; A H Geeraerd; J F Van Impe; J Debevere

doi:10.1046/j.1472-765x.2003.01340.x

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PubMed · 12803551

Modelling the individual cell lag phase. Isolating single cells: protocol development.

K Francois·F Devlieghere·A R Standaert·A H Geeraerd·J F Van Impe·J Debevere

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Abstract

AIMS: To develop a protocol to isolate single cells in wells of a microtitre plate, having a high certainty of individual cells, combined with a sufficient yield. METHODS AND RESULTS: Single cells were obtained using 1/2 dilution series in microtitre plates. Seventy-two Lactococcus lactis dilution series were checked by plate counting. When the last five columns of the plates were observed, the chance of having one single cell was 80%, while the yield was 75 wells containing cells. A simulation model confirmed these results. This method was compared with the commonly applied method. CONCLUSIONS: This method makes it possible to combine a higher chance of having one cell in a microtitre well with a slightly higher yield. SIGNIFICANCE AND IMPACT OF THE STUDY: A tool is developed to isolate single cells to provide a suitable base for investigating and modelling the individual cell lag phase.

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K Francois, F Devlieghere, A R Standaert, A H Geeraerd, J F Van Impe, J Debevere. 2003. Modelling the individual cell lag phase. Isolating single cells: protocol development.. https://doi.org/10.1046/j.1472-765x.2003.01340.x

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