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K Inomata

Publications and source records attributed to K Inomata.

113 records · Page 7Linked to original sources
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[Biological monitoring of exposure to alkylating xenobiotics by determining them in complexes with plasma proteins, hemoglobin, mercapturic acids from urine of rats and industrial workers. I. Acrylonitrile].

A parent molecule of acrylonitrile (cyanoethylene, CE) may be isolated from its the model compound S-(2-cyanoethylene)-1-cysteine, as well as from its N-acetyl derivative and hemoglobin (Hb) and plasma proteins from the intoxicated animals and workers engaged in manufacturing synthetic rubber made from acrylonitrile. The developed procedure consists in the 1.5% H2O2 oxidation of a CE adduct for 30 min, followed by addition of a sample into the gas chromatographic injector at 250 degrees C, resulting in the release of CE from the adducts and in the current ingress of carrier gas into the capillary column in order to separate and determine with a FTD detector. There was a dose-response relationship between the formation of CE adducts with rat blood proteins, urinary mercapturic acids when the poison was given in a dose of 1-50 mg/kg. CE covalently bound to Hb was detected in the workers exposed to its monomer in working places.

Acetylcysteine↗
PubMedDate not supplied

[Biological monitoring of exposure to alkylating xenobiotics by determining them using a new analytical approach in complexes with hemoglobin, plasma proteins and mercapturic acids in urine. II. Acrylamide].

A new simple and prompt procedure for measuring acrylamide (propenamide, PA) in PA-derived mercapturic acid, such as N-acetyl-S-(2-propenamide)-L-cysteine (N-Ac-PAC), and in hemoglobin (Hb) and plasma protein adducts in the rats expose to PA was developed, by employing gas chromatography (GC). PA in mercapturic acids or proteins was liberated on high-temperature heating in an injector port during the working procedure of a GC capillary after preoxidation of sulfur atoms in PA-bound cysteine to a sulfoxide form with hydrogen peroxide and analyzed. This method resulted in 87% of PA in authentic N-Ac-PAC. The number of PA released from the protein adducts was proportional to the cumulative dose of PA given at a concentration of 1-75 mg/kg during 1-3 weeks. The alkylating level of Hb was approximately 65 times higher than that of plasma proteins and it was nearly 6.5% of its cumulative dose. The index of binding to Hb and the rate of its alkylation were 163 and 0.75.10(-3) liter/g-1/hour-1, respectively.

Acetylcysteine↗