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Biomedical subjects

K Rak

Publications and source records attributed to K Rak.

At least 37 records · Page 2Linked to original sources
PubMed1990

The use of polybrene for heparin neutralization in protein C activity assay.

The protein C activity assay of Francis and Patch (Thromb Res 1983; 32: 605-613) is based on the prolongation of the activated partial thromboplastin time in the presence of activated protein C isolated from the test samples. The assay was modified and standardized by Rapaport et al. (Am J Clin Pathol 1987; 87: 491-497), but could still only be used in patients on heparin therapy after chromatographic removal of the heparin. In this study we attempted to eliminate the heparin separation step without losing the advantages of the modified (Rapaport) method. Heparin was added to the isolated protein C to obtain a rapid and complete antithrombin effect after the thrombin activation step and polybrene was subsequently used to neutralize the excess heparin. Using this modified assay protein C activity ranged from 67 to 133% in the normal population, and from 9 to 25% in coumarin-treated patients. Precision of the modified method was acceptable in both normal and pathological PC ranges: within- and between-batch variations were 5.6 and 3.6%, and 8 and 14%, respectively. The assay correlated well (r = 0.84) with the ELISA technique in both healthy donors and non-coumarin-treated patients.

Biological Assay↗
PubMed1989

Congenital factor XIII deficiency with multiple benign breast tumours and successful pregnancy with substitutive therapy. A case report.

A 34-year-old woman with congenital factor XIII (FXIII) deficiency and multiple connective tissue tumours is reported. The subunit a of FXIII was totally absent in her plasma, platelets and histiocytes of breast fibroadenomas and considerably reduced in the monocytes (below 5%). The plasmatic level of subunit b was also reduced (25%). She had a bleeding tendency and habitual abortions. Fresh frozen plasma therapy permitted a successful pregnancy.

Adult↗
PubMed1987

Plasma thromboxane and prostacyclin metabolite ratio in atherosclerosis and diabetes mellitus.

Thromboxane and prostacyclin metabolite determinations (radioimmunoassay) were performed in obliterative atherosclerosis and in diabetes mellitus with microangiopathy. The shift of these metabolites to the thromboxane side could have been documented in both diseases. This phenomenon calls attention to an increased platelet activation and endothelial cell damage. In a third group patients received aspirin (500 mg on alternative days) which caused a marked inhibition of both thromboxane and prostacyclin production, measured this way. The possible role of altered balance of these two prostanoids in atherogenesis and diabetic angiopathy is discussed.

6-Ketoprostaglandin F1 alpha↗
PubMed1986

Differentiation between chronic lymphocytic leukaemia (B-CLL) and non-Hodgkin lymphomas in the leukaemic phase by the mouse red blood cell rosette assay.

A distinction between B-CLL and other malignant B-cell lymphomas in the leukaemic phase may be difficult. Mouse red blood cell rosette formation of lymphocytes from 97 patients with B-CLL and 19 patients suffering from other B-cell lymphoproliferative disorders was examined together with lymphocyte rosette formation of healthy controls. The majority of circulating lymphocytes of B-CLL patients formed rosettes with mouse red cells, whereas there was no relationship between the number of peripheral neoplastic B-cells and that of rosette forming cells in other lymphoproliferative diseases. The relatively simple mouse red blood cell rosette assay proved to be of value in the differentiation of otherwise nearly related conditions.

Adult↗
PubMed1986

Platelet function studies in myeloproliferative disorders.

Platelet functions were studied in 64 patients with various myeloproliferative diseases. The characteristic alterations were prolonged bleeding time, decreased platelet aggregation (but normal results induced by ristomycin), elevated level of BTG, high production of MDA, increased level of TXB2 with almost normal level of 6-keto-PGF1. However, considering the bleeding time and the amount of BTG in relation to the whole blood platelet count, no differences could be detected.

6-Ketoprostaglandin F1 alpha↗
PubMed1986

History of patients with chronic granulocytic leukaemia observed in 1974-1984.

138 CGL-patients were treated in 1974-1984. The female-male ratio was 1.26. In the majority (82.3%) of the 68 patients having died at the blastic phase, all three phases of the disease could be followed. The chronic phase lasted for 31 months. The accelerated disease was detectable in 56 patients, with a mean duration of 5.3 months. The blastic phase, lasting for 4.1 months, was morphologically heterogeneous. Using adequate methods, several groups could be distinguished, i.e. myeloid in 38.3, promyelocytic in 2.9, myelomonocytic in 19.1, megakaryoblastic in 14.7, lymphoblastic in 13.2 and mixed blastic in 11.8%. 23 patients died prior to the development of the blastic phase (9 in the chronic phase, 12 in the accelerated phase and two with chronic neutrophilic leukaemia). The mean survival was in accordance with that in the literature, i.e. 40.6 months. The shortest survival was shown by the lymphoblastic group with the difference manifesting already in the chronic phase. Females survived 13-14 months longer than did males.

Adolescent↗
PubMed1985

Platelet insulin receptor determination in non-insulin dependent diabetes mellitus.

The platelet membrane insulin receptors of healthy and non-insulin dependent (type 2) diabetic patients were studied. Receptor number and affinity proved to be decreased in type 2 diabetes mellitus. The changes in platelet insulin receptor characteristics are in good correlation with the alterations reported in other tissues or cells. The possible role of these phenomena in the pathogenesis of disturbed platelet function in diabetics needs further investigation.

Blood Platelets↗
PubMed1985

Aggristin (ristomycin) precipitation test: a new tool for the detection of fibrin monomer and fibrin degradation products.

The specific detection of fibrin monomer and fibrin degradation products is of high importance in the laboratory diagnosis of intravascular clotting (disseminated intravascular coagulation, deep vein thrombosis). The methods proposed until now are partly time-consuming, needing special laboratories or insensitive and poorly specific. Applying ristomycin instead of ristocetin (another member of the vancomycin antibiotics) a new simple, specific and sensitive method has been elaborated and recommended for the laboratory diagnosis of intravascular coagulation and its differentiation from primary fibrinogenolysis. The results obtained from in vitro and animal experiments and from human studies are presented.

Animals↗
PubMed1985

Cytochemical examination of acid phosphatase and beta-glucuronidase enzymes in low-grade B cell non-Hodgkin's lymphomas.

The differential diagnostic significance of acid phosphatase and beta-glucuronidase were studied in 77 cases of low-grade B cell non-Hodgkin's lymphomas. In most cases the results of cytochemical enzyme studies performed on malignant cells of the bone marrow were evaluated. B cell chronic lymphocytic leukaemia, centrocytic and centroblastic/centrocytic lymphomas were characterized by a weak or a negative acid phosphatase and beta-glucuronidase activity. Stronger positivity was observed in immunocytoma and in Waldenström's macroglobulinaemia, while the highest activity was found in multiple myeloma. Hairy cell leukaemia of B cell origin showed intensive tartrate-resistant acid phosphatase activity. The cytochemical examination of these lysosomal enzymes may be useful in the diagnosis of low-grade malignant lymphomas of B cell origin by completing other methods.

Acid Phosphatase↗
PubMed1985

Study of platelet-associated-immunoglobulins in lymphoproliferative disorders.

In the development of thrombocytopenia in patients with lymphoproliferative disorders immunological factors may play the decisive role. Platelet-associated immunoglobulins were investigated by anti-human IgG and anti-human IgM-coated polyacrylamide beads ("Immunobeads") with light microscopy. Among the 65 patients studied 19 had thrombocytopenia and four of them had an increased number of platelet-immunobead rosettes. In four cases from the group of patients with normal platelet count this phenomenon was also demonstrated. The platelet-associated immunoglobulin was IgG except of one case in which it was IgM. Studying of platelet-associated immunoglobulins might have significance first of all in the choice of the most adequate therapy.

Blood Platelets↗