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Biomedical subjects

K Rak

Publications and source records attributed to K Rak.

49 records · Page 3Linked to original sources
PubMed1984

Detection of platelet-associated IgG in chronic immune thrombocytopenic purpura using antibody-coated polyacrylamide beads.

Platelet-associated IgG (PAIgG) was detected by means of anti-human IgG coated polyacrylamide beads ("Immunobeads") technique in 32 patients with chronic ITP. Both a direct test (with in vivo sensitized platelets) and an indirect test (with in vitro loaded platelets) were carried out. The percent of rosette forming beads was both in the direct test (41.2%) and in the indirect test (32.6%) significantly higher in the cases of chronic ITP patients than in the controls (2.5% and 3.2%, respectively). These results confirm the diagnostic value of this new, relatively simple and rapid method in routine clinical practice.

Acrylic Resins↗
PubMed1984

Study of cell-surface markers in chronic lymphocytic leukaemia (CLL).

There are new possibilities to characterize lymphocytes of the so-called immunoproliferative disorders, e.g. chronic lymphocytic leukaemia. Cell marker studies help to phenotype and differentiate several entities within the CLL-syndrome. Test of rosette formation with mouse erythrocytes together with the surface membrane immunoglobulin detection by immunofluorescence technique proved to be very useful in diagnosing B-CLL and in distinguishing it from other B-lymphoproliferative disorders. In three patients with atypical B-CLL the number of dual-marked (D)-lymphocytes were increased significantly. The prognostic value of this phenomenon is still uncertain.

Animals↗
PubMed1983

Plasma levels of beta-thromboglobulin and factor VIII-related antigen in diabetic children and adults.

In order to investigate the relationship between the in vivo platelet activation in diabetes mellitus and the endothelial damage connected with the diabetic micro- and/or macroangiopathy, plasma levels of beta-thromboglobulin (B-TG) and of factor VIII-related antigen (VIII R:Ag) were studied (1) in juvenile-onset (Type I) diabetics without clinical signs of angiopathy (age under 12 years) and (2) in mostly maturity-onset (Type II) diabetics with and without overt angiopathy (age between 14 and 60 years). Normal controls and nondiabetics with atherosclerosis were also studied. Plasma levels of both proteins were found to be elevated in all the groups of diabetic and atherosclerotic patients in comparison with the controls. Highest levels were found in adult diabetics with angiopathy and in atherosclerotics even without diabetes, but values of the diabetic children were also elevated. The data suggest a causal relationship between the vascular damage and the enhanced platelet reactivity in which the former may play the primary role.

Adolescent↗
PubMed1983

Differential diagnostic value of acid phosphatase and beta-glucuronidase in acute leukaemia.

Differential diagnostic importance of acid phosphatase and beta-glucuronidase reactions was studied in bone marrow smears of 52 patients with acute leukaemias. Both reactions showed either diffuse or simultaneously diffuse and granular positivity in the medullary blast cells of 34 patients suffering from ANLL. A strong diffuse positivity of acid phosphatase suggested the possibility of AMOL. Beta-glucuronidase and acid phosphatase reactions were exclusively granular in every positive case of ALL. Increased acid phosphatase activity was found in T-ALL while beta-glucuronidase showed increased activity also in (non-T, non-B)-ALL on several occasions.

Acid Phosphatase↗
PubMed1983

Lymphocytes bearing both T and B markers in lymphoproliferative disorders.

The appearance of dual-marked (D) lymphocytes, i.e. lymphocytes having both T and B markers, was investigated in patients with acute (ALL) and chronic lymphocytic leukaemia (CLL) as well as with non-Hodgkin's lymphoma (NHL). E-rosette and immunobead-rosette techniques were combined and simultaneously administered, and the combination was found to be simple, fast and reproducible. D cells could be detected also in normal peripheral blood, however, only in a low percentage. In the blood of 4 patients suffering from ALL, in 2 with CLL and in 1 with NHL the number of D cells were increased significantly. In ALL the relative number of these cells decreased during remission and increased in relapses; thus the determination of the relative frequency of D cells might have prognostic value and could be useful in the choice of an optimal therapeutic protocol.

Adult↗
PubMed1981

Congenital deficiency of cyclo-oxygenase in a woman with generalized atherosclerosis.

The case of a 52-year-old woman with congenital cyclo-oxygenase deficiency, signs of generalized atherosclerosis and a moderate bleeding tendency is reported. Secondary platelet aggregation was absent. Platelet aggregation induced by arachidonic acid failed totally while that induced by calcium ionophore was normal. No malondialdehyde formation could be detected in her platelet-rich-plasma. The life-long deficiency of cyclo-oxygenase had not protected her from progressive vascular disease. This case suggests that the chronic intake of large doses of aspirin cannot prevent arterial disorders.

Arteriosclerosis↗