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Biomedical subjects

K Stoltze

Publications and source records attributed to K Stoltze.

At least 37 records · Page 2Linked to original sources
PubMed1993

Variables related to masseter muscle function: a maximum R2 improvement analysis.

A multiple linear regression analysis, with stepwise maximum R2 improvement technique by forward selection and pair switching, was used to select the occlusal, morphologic, and histologic variables which explained most of the variation in bite force and electric masseter muscle activity. The variables comprised tooth contact and facial morphology together with thickness and fiber characteristics of the masseter muscle. The study included 13 healthy women, 21-28 yr of age, with a minimum of 24 teeth and no serious malocclusion. Significant exploratory models (R2: 0.55-0.85) were shown concerning bite force, and electromyographic amplitude during resting posture, maximal voluntary contraction (ICP), and unilateral chewing, as well as contraction time (chewing side). Muscle thickness and molar contact had a significant, positive effect on the level of forceful muscle contraction. The explorative model both demonstrated explicable relations, and offered better insight into interrelations than did univariate analysis.

Adenosine Triphosphatases↗
PubMed1992

Systemic absorption of metronidazole after application of a metronidazole 25% dental gel.

Systemic absorption of metronidazole was studied after one application of a metronidazole 25% dental gel into inflamed periodontal pockets. 14 patients with periodontal disease participated. Metronidazole gel was applied into the pockets of all teeth with probing depth > or = 5 mm, minimum 10 teeth/patient. Excess gel was carefully collected in order to calculate the amount of metronidazole applied into the pockets. Blood samples for analysis of metronidazole in plasma were taken before and up to 72 h after application. To determine the bioavailability of metronidazole administered as gel, the patients were later given an i.v. dose of 100 mg metronidazole. Metronidazole was quantified by HPLC. The mean actual dose of metronidazole in gel was 55 mg (SD: 24 mg, range: 29-103 mg). Allowing for the fact that excess gel corresponding to about 60% of the applied amount of metronidazole under normal clinical conditions may be swallowed, the systemic load after one application of metronidazole 25% dental gel will still be less than after one metronidazole 250 mg tablet. Peak plasma concentrations varied between 223 and 1303 ng/ml (mean: 581 ng/mk, SD: 320 ng/ml) and were reached within 2 to 8 h (mean: 4.4 h). The mean bioavailability of metronidazole dental gel was 71%.

Absorption↗
PubMed1992

Concentration of metronidazole in periodontal pockets after application of a metronidazole 25% dental gel.

The metronidazole concentration was monitored in gingival crevicular fluid (GCF) of inflamed periodontal pockets after 1 application of a 25% gel. 12 patients with periodontal disease were included in the study. Metronidazole gel was applied with a syringe into the pockets of 10 teeth with probing depths > or = 5 mm. Samples of GCF were taken with Periopaper before application and 4, 8, 12, 24 and 36 h after application. At each time, samples from 2 teeth were pooled in order to obtain a sufficient amount of fluid for analysis. By means of a calibrated Periotron, the collected volume of GCF was measured. High-performance liquid chromatography was used to determine the amount of metronidazole. MIC50 for anaerobic periopathogens susceptible to metronidazole is below 1 microgram/ml. In this study, the concentration obtained was higher than 1 microgram/ml in all samples after 4 and 8 h, in 92% after 12 h, in 50% after 24 h and in 8% after 36 h. Thus, metronidazole concentrations in the pockets were generally above MIC50 for susceptible periopathogens 24 h after 1 application of a 25% metronidazole gel.

Adult↗
PubMed1992

3 regimens of topical metronidazole compared with subgingival scaling on periodontal pathology in adults.

This report presents the findings from an open randomized multicentre clinical trial designed to compare the clinical efficacy of scaling with application of 3 different preparations/dose frequencies of topical metronidazole in the treatment of adult periodontitis. The 4 treatments were: (A) metronidazole 25% dental gel administered 1 x a week for 2 weeks; (B) metronidazole 15% dental gel applied 1 x a week for 2 weeks; (C) metronidazole 15% dental gel applied 2 x a week for 2 weeks; (D) subgingival scaling, performed 1 x only. A split mouth design was used. Patients were included in the study if they had at least 1 tooth in each quadrant with a pocket depth > or = 5 mm in at least 1 of 4 sites. A total of 61 patients from 4 centres were entered into the study. The efficacy parameters were probing pocket depth and bleeding on probing. Follow-up visits for recording of clinical efficacy were made at 2, 4, 6 and 12 weeks after the end of metronidazole treatment. All 3 antibiotic treatments (A, B, C) reduced the symptoms of periodontal pathology and yielded results comparable to those seen after subgingival scaling (D). When using a topical drug therapy, it seems important to use a preparation that requires as few applications as possible. The best candidate for drug therapy would therefore be treatment (A) metronidazole 25% applied 1 x a week for 2 weeks.

Adult↗
PubMed1992

Clinical responses to subgingival application of a metronidazole 25% gel compared to the effect of subgingival scaling in adult periodontitis.

A newly developed metronidazole 25% dental gel was compared with subgingival scaling in the treatment of adult periodontitis. 206 patients in 9 centres participated in the study. Probing pocket depth (PPD) and bleeding on probing (BOP) were recorded before treatment and 2, 6, 12, 18, and 24 weeks after the treatment. All patients had at least 1 tooth in each quadrant with a PPD of 5 mm or more. The treatments consisted of 2 applications of dental gel (days 0 and 7) in 2 randomly selected quadrants (split mouth design) and 2 sessions of subgingival scaling (1 quadrant on day 0, and 1 quadrant on day 7). Instruction in oral hygiene was given 2 weeks after completed treatment. The average PPD and the average frequency of BOP were calculated over all sites with initial PPD of 5 mm or more. PPD and BOP were thus, at each examination, calculated from the same sites. The mean PPD was 5.9 mm before gel application and 5.8 mm before scaling (p = 0.31). BOP was 88% in both treatment groups. 24 weeks after the treatment, PPD and BOP were significantly reduced in both groups and for both parameters (p < 0.01). PPD was reduced by 1.3 mm after gel application and 1.5 mm after scaling; BOP was reduced by 32% and 39%, respectively. The difference between the treatments was statistically significantly, but considered as clinically unimportant.

Adult↗
PubMed1989

Immunohistochemical distribution of keratin proteins in clinically healthy human gingival epithelia.

In clinically healthy/subclinically inflamed biopsies of marginal gingiva, the immunohistochemical distribution of keratin proteins was studied in junctional (JE), sulcular (SE), oral gingival (OGE) and in a few samples of alveolar mucosal epithelium (AE) by means of various mouse monoclonal anti-keratin antibodies in an indirect fluorescence technique. All regions stained in a nearly similar way with AE3 (keratins 1-8, all cells) and BE14 (keratin 5, basal and supra/parabasal cells). AE8-staining (keratin 13, supra/parabasal and spinous cells) was primarily confined to the stratified, nonkeratinized epithelia SE and AE, but also a variable part of JE and less frequently OGE were positive. The parakeratinized OGE was distinct in showing a homogeneous staining with AE2 (keratins 1/2, 10) and AE5 (keratin 3) throughout spinous cell layers. These antibodies did not stain JE and AE whereas SE stained in a scattered way with AE5 and sometimes also with AE2. The latter finding might indicate initial keratinization at molecular level. The JE was distinct in retaining basal characteristics throughout the epithelium with PKK2 (keratin 7, 16, 17, 19) and BE14 (keratin 5) although some initial suprabasal maturation, as observed with AE8, cannot be excluded. Differences in keratin staining of gingival epithelia and the AE was found with respect to AE1-reactivity (keratins 10, 14-16, 19) which was suprabasal in JE, SE and OGE but basal in AE.

Adolescent↗
PubMed1989

Behavior of in vitro grown normal human mucosal epithelial cells and tumorigenic rat cells inoculated into nude mice.

The present study describes the behavior of in vitro grown normal human oral mucosal epithelial cells and that of a tumorigenic epithelial cell line following subcutaneous inoculation into nude mice. A successful recovery of viable human epithelial cell inocula was seen in 25-90% of mice and there was no improvement in recovery rates after addition of fibroblasts. These inocula resulted in cyst formation lined by a 2-6 cell layer unkeratinized squamous epithelium without rete ridges. There was no increase in recovery rate or size of cysts when coinoculated with fibroblasts. The tumorigenic cell inocula were successfully recovered in all cases. Tumors established from these inocula had a low grade of differentiation and were without signs of metastasis. Inocula of tumorigenic cells showed an increased size after addition of fibroblasts to the inocula. The model may be useful in studies of interactions between inoculations of heterologous normal and pathologic cells as well as in studies of differentiation of carcinogen-treated epithelial cells.

Animals↗
PubMed1989

Human pulp reactions to resin restorations performed with different acid-etch restorative procedures.

Fifty-eight experimental resin restorations were performed in intact, human premolars, using different leakage-reducing restorative procedures. These were conventional acid-etching and acid-etching followed by cavity treatment with an intermediary layer of low-viscous resin or the dentin adhesive NPG-GMA/ethanol. The teeth were extracted after 4 months and examined for pulpal inflammation/necrosis (I), reduction of odontoblasts (OR), and formation of tertiary dentin (TD). By the general linear model procedure, 91%, 34%, and 56% of the variations in I, OR, and TD, respectively, could be explained by variations in the experimental conditions. The significant independent variables were jaw, stage of root formation, width of pulp, width of cavity, marginal leakage, bacteria in the cavity, bacteria in the exposed dentinal tubules, and the restorative procedure. With regard to the restorative procedure the analyses showed that application of low-viscous resin increased the pulpal reactions OR and I, whereas cavity treatment with NPG-GMA/ethanol had no adverse biologic effect.

Acid Etching, Dental↗
PubMed1988

Blackening of unprotected dental X-ray films due to scattered radiation.

Unexposed films awaiting exposure as well as exposed films awaiting processing are subjected to scattered radiation if kept unprotected in the dental X-ray clinic. The purpose of the present study was to analyze the influence of various storing principles, film speed, and distance from X-ray source upon the degree of film blackening. Test films were subjected to scattered radiation from 150 exposures. Maximum additional blackening (0.27 D) was recorded for type E films not protected by lead foil at the shortest distance studied (55 cm). At a distance of 200 cm blackening was reduced to 0.02 D and could be further reduced by utilizing the inherent protective effect of the lead foil. It is concluded that if dental X-ray films not in use are kept at a distance of 200 cm from the X-ray source and protected by lead foil additional blackening due to scattered radiation is negligible and further protective precautions are unnecessary.

Lead↗
PubMed1988

Distribution of Langerhans cells in clinically healthy human gingival epithelium with special emphasis on junctional epithelium.

Twenty-one biopsies of clinically healthy marginal gingiva from children, who performed conventional oral hygiene but received no additional professional prophylaxis, were studied in order to obtain information on distribution and density of Langerhans cells (LC) in the oral gingival epithelium (OGE), the sulcular epithelium (SE) and the junctional epithelium (JE). A simple freeze-separation technique was found to create acceptable histomorphology of JE in specimens obtained adherent to teeth, while partially and non-adherent ones were rejected. The majority of LC in OGE were highly dendritic and stained intensively with OKT6 monoclonal antibodies. The distribution was network-like with a density of 21.0 +/- 3.2 LC/0.1 mm2 cross-sectional epithelial area. A similar although less dense distribution was found in SE (8.6 +/- 3.0 LC/0.1 mm2). These observations confirm previous findings. In JE 2 groups of LC were identified: 1) Weakly stained LC with very few and short dendrites distributed in a scattered way (2.8 +/- 1.4 LC/0.1 mm2) in the apical three-fourths of JE in most specimens. Present evidence suggests that these cells might be immature cells of Langerhans lineage. 2) Clusters of LC (9.4 +/- 2.9 LC/0.1 mm2) with dendrites of moderate lengths and numbers and a varied fluorescence intensity; they were found in a few specimens in the coronal one-fourth of JE and at the border zone to SE. Such clusters might represent genuine variation in the distribution of LC or reactions to initial/early plaque formation.

Adolescent↗
PubMed1987

Influence of projection angles upon position of cementoenamel junction on radiographs.

The CEJ on radiographs is often used as a reference point in the assessment of alveolar bone loss. The aim of the present study was to test the stability of the radiographic CEJ ("RCEJ") in relation to the alveolar crest under varying angulations of the central X-ray beam. In an experimental model four types of extracted teeth were radiographed at 0 degree and 20 degrees vertical angulation and at 15 horizontal angulations varying from a 17.5 degrees mesial view to a 17.5 degrees distal view. The buccal alveolar bone margin was simulated by a steel needle. RCEJ-"crest" distances were read at a X 10 magnification. Changing the vertical angulation from 0 degree to 20 degrees reduced the RCEJ-"crest" distances for all tooth types (range of average reduction 2.2-4.1 mm). Horizontal angulations without concomitant vertical angulation had a significant effect but were without clinical significance. Horizontal angulations in combination with a 20 degrees vertical angulation had a considerable influence; in a mesial view, mesial RCEJ-"crest" distances decreased and distal ones increased, and vice versa in a distal view. The deviations from the true CEJ-crest distance demonstrated may be of clinical significance, and a critical attitude to alveolar bone loss measures on radiographs using the RCEJ as reference point is recommended.

Alveolar Process↗
PubMed1981

Human buccal mucosa transplants in nude mice.

The development of a model to study tissue interactions in human oral mucosa in vivo utilizing transplantation of human tissue to nude mice is described. Ninety-six samples of normal human buccal mucosa were transplanted to subcutaneous sites in the flank region of nude mice and were protected from overlying cutaneous and subcutaneous tissue by polyethylene capsules or by Millipore filters. Transplant recovery rates were 47% an 69%, respectively. The histologic features of the epithelium of transplanted specimens were compared to 20 biopsies of normal buccal mucosa. The epithelium of transplants maintained for 3 weeks or longer with either type of protection, maintained features essentially similar to the normal controls but was somewhat thinner.

Animals↗
PubMed1981

Epithelial outgrowths from human buccal mucosa transplants in nude mice.

The histologic features of epithelial outgrowths from transplants of human buccal mucosa in nude mice are described. Nine-six samples of normal buccal mucosa were transplanted to subcutaneous sites in the flank region of nude mice. The tissue specimens were maintained in the mice for periods ranging from 1 to 67 d and were protected from overlying murine cutaneous and subcutaneous tissue by polyethylene capsules or by Millipore filters. The epithelial outgrowth from the transplants were formed over murine connective tissue, with and without substantial infiltrate of neutrophils, and over Millipore filter with and without interjacent murine connective tissue. The stratified squamous epithelium was essentially unkeratinized and thinner than that overlying originally transplanted connective tissue. The histologic features of the outgrowths were similar irrespective of the technique used or the type of underlying tissue.

Animals↗
PubMed1980

Effect of toothbrush stimulation on temperature of gingival and alveolar mucosae.

Thermometry was used to study the effect of applying a toothbrush to three types of oral mucosae, in forty dental students. Brushing with an electric tooth brush resulted in an immediate fall in temperature, but 3 min later the temperature was significantly higher than before stimulation. The temperature changes were greater in gingival crevice and attached gingiva compared with alveolar mucosa. Moreover, it was found that a static application of the toothbrush produced temperature changes which were equal to those following a dynamic application.

Adult↗
PubMed1980

Host response to two different designs of minor connector.

Temperatures were recorded in thirty-two gingival crevices adjacent to proximal surfaces of abutment teeth fitted with minor connectors of an open space design, and in thirty gingival crevices adjacent to proximal surfaces fitted with minor connectors of a closed design. During the 2 week experimental period, the twenty-seven removable partial denture subjects who were involved in the study kept the accumulation of plaque to a minimum by applying specialized tooth-brushing. Although an equal but small amount of plaque formed in both groups, the presumed minimal rise in temperature stimulated by this plaque did not prevent the two connector designs from showing disparate effects on the crevicular temperature. It was concluded that the open space design of minor connector is less conducive to irritation of the adjacent marginal gingiva than is the closed design.

Dental Plaque↗