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Biomedical subjects

L A Devriese

Publications and source records attributed to L A Devriese.

At least 19 recordsLinked to original sources

Characteristics of bovine Staphylococcus aureus with special regard to clumping factor activity.

The occurrence of the following groups of bovine Staphylococcus aureus with characteristic patterns of biochemical reactions and sensitivities to phages was confirmed by the present investigation of 662 isolates from the udders of cows in 53 dairy herds: (1) host-specific variety (v) bovis, (2) not alloted strains with the basic marker, crystal violet type A (group na CV A), (3) group na CV C. The latter was split into three subgroups clearly differing in phage susceptibility patterns: I/119, I without 119 and III, respectively. Lack of clumping factor activity occurred in 323 out of 346 (93%) phage pattern I/119 strains examined while most or all isolates of the other groups of bovine strains proved to be clumping-positive.

Animals

Enterotoxin production in different Staphylococcus aureus biotypes isolated from food and meat plants.

Strains of Staphylococcus aureus isolated in Belgium and Zaïre from food and from various sources in the meat industry were biotyped, phage typed and tested for staphylococcal enterotoxin (SE) production. Thirty of the 185 strains examined produced one or more SE, and 23 of these belonged to the human biotype. Most SE-positive strains belonged to phage groups III and Mixed, or were not typable. None of the poultry-like biotype strains, which were frequent in nasal carriers among workers in meat plants as well as in minced meat, produced enterotoxins. Avian biotype strains similarly were negative.

Abattoirs

Enterococcal and streptococcal species isolated from faeces of calves, young cattle and dairy cows.

Faeces from non-ruminating calves were found to contain several species of enterococci: Enterococcus avium, Ent. cecorum, Ent. durans, Ent. faecalis, Ent. faecium and Ent. hirae. Enterococcus faecalis was the most frequent. Few of these animals carried streptococci. Streptococcus bovis largely predominated in ruminating calves, young cattle and dairy cows. Other streptococci as well as enterococci were infrequent in dairy cows, but a variety of other streptococci and enterococci were found in the faeces of young ruminating animals.

Age Factors

Identification and composition of the tonsillar and anal enterococcal and streptococcal flora of dogs and cats.

Enterococcus faecalis was the most frequently isolated enterococcal species from anal swabs and tonsils of dogs and cats, although in the anal samples from dogs Ent. hirae was found almost as often as Ent. faecalis. Most Ent. faecium strains from dog tonsils differed from those associated with humans and other animals in that they fermented sorbitol. Typical Ent. avium as well as atypical Ent. avium-like strains were seen in dogs, while the related species Ent. raffinosus was associated with cat tonsils. Enterococcus cecorum also occurred mainly in cats. Certain atypical strains, presumptively identified as Ent. cecorum, shared characteristics with Ent. columbae. The most frequent streptococcal species in tonsils of cats and dogs were Streptococcus suis and Strep. canis. Streptococcus canis and Strep. bovis predominated in anal swabs. The canine Strep. suis differed from the common porcine strains in fermenting mannitol. Forty-seven of the 288 isolates examined could not be identified or related to known species. The characteristics of two groups of these bacteria, provisionally called 'Ton 31 group' and 'O7 group' are described.

Anal Canal

Biochemical and antigenic properties of Streptococcus bovis isolated from pigeons.

Biochemical and serological properties of 60 strains of Streptococcus bovis isolated from healthy pigeons and from pigeons that died from S. bovis septicemia were determined. On the basis of the hemolysis of bovine erythrocytes, the production of polysaccharides on saccharose-containing media, and the fermentation of mannitol, inulin, trehalose, and L-arabinose, the isolates were classified in five biotypes and two subbiotypes. Slide agglutination and microagglutination tests using monospecific rabbit antisera allowed the classification of all isolates in five serotypes.

Animals

Efficacy of tilmicosin in treatment of pulmonary infections in calves.

The efficacy of tilmicosin in the treatment of respiratory infections in calves was evaluated. According to a randomised block design, 58 calves with naturally occurring respiratory infections were treated with one of the following products: a single subcutaneous injection of tilmicosin (10 mg/kg liveweight) or daily intramuscular injections of 5 mg lincomycin and 10 mg spectinomycin/kg liveweight, for a minimum of three days. Both treatment groups initially showed similar clinical signs and their initial responses to the treatments were good. However, the tilmicosin treated calves improved more rapidly. Significantly greater improvements (P less than or equal to 0.05) were observed in their demeanour and appetite during the first 10 days after treatment began, and in their respiratory condition between five and 10 days after treatment began.

Animals

Genetic transformation of various species of Enterococcus by electroporation.

A transformation system for Enterococcus faecalis was developed which uses untreated (i.e., non-protoplasted) cells and the electroporation technique. The optimized protocol resulted in transformation efficiencies of up to 4 x 10(6) transformants per microgram of plasmid DNA. All strains of E. faecalis tested could be transformed by this method, albeit with differing transformation efficiencies. Using the protocol optimized for E. faecalis we successfully transformed Enterococcus faecium, E. hirae, E. malodoratus and E. mundtii.

Cell Division

Protective effects of vaccines against experimental salmonellosis in racing pigeons.

Five inactivated and one attenuated vaccine produced for the prevention of salmonellosis in pigeons were compared in an experimental challenge model. The birds were vaccinated according to the recommendations of the manufacturers and they were infected by gavage with a Salmonella typhimurium (var copenhagen) pigeon strain. The challenged control animals showed severe weight loss, excessive water intake over a prolonged period, and excreted large numbers of salmonellae. None of the vaccines fully protected the pigeons, and only an inactivated oil adjuvant vaccine was able to reduce the severity of the clinical signs significantly. Mortality was low and tended to increase with the severity of the clinical signs. These results do not justify the preventive use of salmonella vaccination in pigeons. Nevertheless, the oil adjuvant vaccine may help in the effective cleaning of lofts after an outbreak of salmonellosis.

Animals

Cutaneous infection associated with Staphylococcus hyicus in cattle.

Forty-seven Staphylococcus hyicus strains, one each from 47 of 81 cattle examined, were isolated from cutaneous lesions on different parts of the body. Nineteen of the strains were isolated in pure culture and the rest were isolated in association with either other staphylococci or microfilariae or mange mites. Three out of 10 representative strains produced cutaneous lesions when inoculated intradermally into rabbits.

Animals

Characterization of Staphylococcus aureus and Salmonella spp. strains isolated from bovine meat in Zaïre.

A large majority (87.4%) of 190 Staphylococcus aureus isolates from fresh beef in Lubumbashi (Zaïre) belonged to the human St. aureus ecovar; 81.2% of the phage-typed human strains were partially or solely lysed by phages of group III. Thirteen of the 52 tested strains (25.0%) were enterotoxin producers; nine of these (69.2%) were positive for staphylococcal enterotoxin A. Sixteen serotypes were identified among the 122 Salmonella isolates and nearly all these strains were susceptible to the 8 different antibiotics tested.

Animals

Incidence and significance of isolation of Mycoplasma felis from conjunctival swabs of cats.

Conjunctival swabs taken from 40 cats with conjunctivitis and 65 cats without conjunctivitis were examined for the presence of Mycoplasma felis. The incidence of M. felis in cats with conjunctivitis was 25%. M. felis was not isolated from clinically normal cats. Inoculation of two isolates into the conjunctival sacs of healthy cats induced conjunctival hyperemia, starting 2-3 days after inoculation and disappearing without treatment within 7 days. It is concluded that M. felis plays a role in feline conjunctivitis.

Animals

Characteristics of different Streptococcus suis ecovars and description of a simplified identification method.

Streptococcus suis strains associated with different animal hosts or located in different body regions constitute special ecovars of S. suis. A group of CO2-dependent strains which showed several unusual traits was found in the genital tract of swine, and mannitol- and sorbitol-fermenting strains were isolated from sheep, cattle and horses. S. suis was found to be biochemically more heterogenous than originally described. A detailed description is given of additional characteristics of the species as well as of characters which should be modified in the species description. A simple scheme which uses the amylase and the Voges-Proskauer (acetoin) reactions for the identification of S. suis is proposed. All S. suis strains tested produced a characteristic amylase reaction on Mueller-Hinton plates or on Columbia Agar Base enriched with starch.

Animals

Enumeration by a miniaturized method of Escherichia coli, Streptococcus bovis and enterococci as indicators of the origin of faecal pollution of waters.

Counts of Escherichia coli, faecal streptococci and enterococci were made on faecal specimens from human and animal origin and urban raw sewage waters, with microtiter plates containing selective substances. Escherichia coli was more numerous than faecal streptococci and enterococci in 80% of the samples regardless of the origin. Consequently the use of the ratio E. coli/faecal streptococci to distinguish human from animal origin of faecal pollution is questionable. Enterococcus faecalis was predominant in human and poultry faeces, Streptococcus bovis was typical of the bovine faeces and to a lesser extent also of pig faeces whereas Enterococcus durans, Ent. hirae and Ent. faecium did not characterize any faecal source. Streptococcus bovis could be distinguished in the microtiter plate by its inability to reduce triphenyl tetrazolium chloride (TTC) in the medium.

Animals