Bacteriophage degradation of Klebsiella K30 capsular polysaccharide. An NMR investigation of the 3,4-pyruvated galactose-containing repeating oligosaccharide.
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Bacteriophages↗
Biomedical subjects
L A Parolis
Publications and source records attributed to L A Parolis.
Structural elucidation of the capsular polysaccharide expressed by Escherichia coli O20:K83:H26 by high resolution NMR spectroscopy.
The structure of the capsular polysaccharide produced by Escherichia coli O20:K83:H26 was investigated by one- and two-dimensional 1H and 13C NMR spectroscopy, and by glycose and methylation analysis. The capsular polysaccharide was shown to be comprised of linear pentasaccharide repeating units with the structure: [formula: see text]
Carbohydrate Conformation↗
Escherichia coli serotype K45 capsular antigen: a glycan containing 3-acetamido-3,6-dideoxygalactopyranose.
The primary structure of the acidic capsular antigen of Escherichia coli O8:K45:H9 was shown by glycose analysis, methylation analysis, and one- and two-dimensional 1H and 13C NMR spectroscopy to be composed of repeating branched tetrasaccharide units having the structure: [formula: see text] The polysaccharide contains the uncommon amino sugar 3-acetamido-3,6-dideoxy-D- galactopyranose, which has not been previously identified in a capsular antigen.
Acetylgalactosamine↗
Klebsiella K43 capsular polysaccharide: primary structure and depolymerisation by a viral-borne endoglycanase.
The capsular polysaccharide of Klebsiella K43 has been studied by glycose analysis, methylation analysis, and NMR spectroscopy, and by bacteriophage depolymerisation of the native polysaccharide. Additional evidence for the structure of the repeating unit came from base-catalysed degradation of the methylated polysaccharide, and from NMR spectroscopic analysis of the lithium-degraded polysaccharide and of the oligosaccharide-alditol derived from the repeating unit oligosaccharide obtained from a bacteriophage degradation. The polysaccharide was shown to have the repeating unit: [formula: see text]
Bacteriophages↗
Structural elucidation of the capsular polysaccharide of Escherichia coli serotype K101 by high resolution NMR spectroscopy.
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Acetylgalactosamine↗
The capsular antigen of Escherichia coli serotype O8:K102:H-.
The structure of the capsular antigen of E. coli O8:K102:H- was investigated by methylation analysis, beta-elimination of the methylated polysaccharide, lithium-ethylenediamine mediated degradation, and by 1D and 2D 1H and 13C NMR spectroscopy of the lithium-degraded and native polysaccharides. The capsular antigen was shown to have the following branched pentasaccharide repeating unit: [formula: see text]
Antigens, Bacterial↗
Structural elucidation of the capsular polysaccharide of E. coli serotype K47.
The capsular polysaccharide from Escherichia coli K47 was investigated using mainly methylation analysis and 1H and 13C NMR spectroscopy and shown to have the following repeating unit: [Formula: see text]
Antigens, Bacterial↗
Re-investigation of the structure of the capsular polysaccharide of Klebsiella K15 using bacteriophage degradation and inverse-detected NMR experiments.
The structure of the capsular polysaccharide from Klebsiella K15 has been re-investigated, principally by 1D and 2D 1H- and 13C-NMR spectroscopy of the oligosaccharide-alditol obtained by depolymerisation of the polysaccharide with a viral-borne endoglycanase followed by borohydride reduction of the isolated repeating oligosaccharide. The capsular polysaccharide was shown to have the repeating unit: [Formula: see text]
Bacteriophages↗
Escherichia coli O9:K38 capsular antigen: another ribofuranose-containing glycan.
The primary structure of the O-deacetylated acidic capsular antigen of Escherichia coli O9:K38 was shown by glycose analysis, methylation analysis, and one- and two-dimensional 1H- and 13C-n.m.r. spectroscopy to be composed of repeating linear pentasaccharide units having the structure.
Bacterial Capsules↗
Structure of the glycocalyx polysaccharide of Pseudomonas fragi ATCC 4973.
The structure of the exocellular glycocalyx polysaccharide of Pseudomonas fragi ATCC 4973, a bacterium implicated in the spoilage of meat, has been determined using hydrolysis, methylation analysis and 1D- and 2D-n.m.r. spectroscopy. The polysaccharide, which aids in the adhesion of the cells to each other and to the meat tissue, has the regular repeating unit ----4)-3-O-[(R)-1-carboxyethyl]-alpha-D-Glcp-(1----3)-beta-D-ManpNAc+ ++- (1----4)-beta-D-Glcp-(1----. Random partial O-acetylation occurred in some preparations of the polysaccharide.
Carbohydrate Conformation↗
The structure of the capsular antigen from Escherichia coli O8:K87:H19.
The structure of the capsular polysaccharide from Escherichia coli O8:K87:H19 was investigated by methylation analysis and by one- and two-dimensional 1H- and 13C-n.m.r. spectroscopy. The repeating unit was shown to be a branched pentasaccharide with the structure (formula; see text)
Antigens, Bacterial↗
A structural investigation of the capsular polysaccharide of Escherichia coli O9:K57:H32.
The primary structure of the acidic capsular polysaccharide of Escherichia coli K57 was elucidated by methylation analysis and 1D- and 2D-n.m.r. spectroscopy. The repeating unit was identified as a linear tetrasaccharide having the structure shown. ----2)-beta-D-Ribf-(1----4)-beta-D-Galp-(1----3)-alpha-D-GlcpNAc-( 1----4)-alpha - D-GalpA-(1----.
Carbohydrate Conformation↗
A re-investigation of the structure of the capsular polysaccharide of Klebsiella K10.
The capsular polysaccharide of Klebsiella K10 was investigated by methylation analysis and 1H-n.m.r. spectroscopy, and the deacetylated bacteriophage-degraded polysaccharide by 1D- and 2D-n.m.r. spectroscopy. The repeating unit was shown to be a branched hexasaccharide (see text). OAc substituents were located on the terminal and 2-linked galactopyranosyl residues by Prehm methylation of a low-molecular-weight fraction obtained by bacteriophage degradation.
Bacteriophages↗
Structure of the capsular antigen of Escherichia coli O8:K8:H4.
The structure of the capsular polysaccharide from Escherichia coli O8:K8:H4 has been elucidated, using mainly methylation analysis, Smith degradation, and 1D- and 2D-n.m.r. spectroscopy. The polysaccharide, after removal of bound lipid, was found to be composed of repeating units of the linear tetrasaccharide. (sequence; see text)
Carbohydrate Sequence↗
Escherichia coli serotype-39 capsular polysaccharide: primary structure and depolymerisation by a bacteriophage-associated glycanase.
The acidic capsular polysaccharide isolated from Escherichia coli O9:K39:H9 was investigated, using n.m.r. spectroscopy, methylation analysis, uronic acid degradation of the native and methylated polysaccharides, and bacteriophage-associated enzyme degradation. The structure of the repeating unit, which is shown below, is identical to that reported for Klebsiella serotype-61 capsular polysaccharide. (formula; see text)
Antigens, Bacterial↗
Preparation of branched hexasaccharides by the action of a viral lyase on Klebsiella K14 polysaccharide.
Klebsiella K14 capsular polysaccharide was degraded by a bacteriophage-borne enzyme to afford oligosaccharides A-C which were studied by one- and two-dimensional n.m.r. spectroscopy. A and B were the repeating-unit hexasaccharide and pyruvylated hexasaccharide, respectively, while C was a dodecasaccharide. Each oligomer was terminated by a reducing mannose and a non-reducing 4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid residue, indicating that the phage enzyme had cleaved the beta-D-Manp-(1----4)-beta-D-GlcpA linkages in the polysaccharide by a lyase, rather than the more common glycosidase, activity found with other Klebsiella bacteriophages. In this respect, the depolymerisation resembles those reported for the capsular polysaccharides of Klebsiella K5 and K64
Klebsiella↗
Primary structure of Klebsiella serotype K22 capsular polysaccharide: another glycan containing 4-O-[(S)-1-carboxyethyl]-D-glucuronic acid.
The primary structure of the acidic capsular polysaccharide isolated from Klebsiella serotype K22 has been investigated using methylation analysis, hydrolysis, bacteriophage-borne enzyme degradation, and n.m.r. spectroscopy. The repeating unit comprises the chain disaccharide----3)-beta-D-Galp-(1----4)-beta-D-Glcp-(1---- substituted by 4-O-[(S)-1-carboxyethyl]-beta-D-GlcpA-(1----6)-alpha-D-Glcp-(1---- at O-4 of the galactose. The galactose carries an O-acetyl group on position 6.
Antigens, Bacterial↗
The use of bacteriophage-mediated depolymerisation in the structural investigation of the capsular polysaccharide from Escherichia coli serotype K36.
The structure of the repeating unit of the capsular polysaccharide from Escherichia coli serotype K36 has been established from the results of spectroscopic and chemical analyses of (a) P1, the tetrasaccharide obtained on depolymerisation of the polysaccharide with a bacteriophage-borne endo-galactosidase, (b) P1-alditol, and (c) the original polysaccharide. The repeating unit, which is identical to that reported for Klebsiella K57, has the following structure. (Formula: see text).
Carbohydrate Conformation↗