Biomedical subjects

L B Deppe

Publications and source records attributed to L B Deppe.

3 recordsLinked to original sources

PubMed1982

Preparation and characterization of the anti-idiotypic properties of rabbit anti-timothy antigen B helper factor and anti-mouse timothy IgE antisera.

with AgB-primed B or T cells and injected into syngeneic X-irradiated recipients. Anti-THF and anti-Eid purified by an AgB-specific T suppressor factor (TSF) affi-gel adsorbent retain their ability to specifically initiate [3H]-thymidine incorporation of AgB-primed T cells. The data indicate that both anti-THF and anti-Eid recognize unique determinants present on AgB-specific T-helper, T-suppressor and B cells, and suggest that the receptors on AgB-specific T and B cells share cross-reactive idiotypic determinants.

Animals↗

PubMed1981

Induction and characterization of suppressor T cells and soluble factors with modified timothy grass pollen AgB.

Previous studies demonstrated that antigen B (AgB), a major antigen of timothy grass pollen, modified by photooxidation (Ox-AgB) does not react with rabbit, human, or mouse antibodies directed against AgB and does not induce antibodies reactive with either native or modified AgB. However, immunization of mice with Ox-AgB in alum induces significant T helper cell activity. In this review, we describe the conditions and kinetics for Ox-AgB induction of T suppressor cells, the secretion of AgB-specific T suppressor factor (TSF), and the partial purification of AgB-specific TSF.

Adsorption↗

PubMed1981

Antigen B-specific helper factor from timothy grass pollen: isolation and partial characterization.

Spleen cells from LAF1 mice primed with photo-oxidized antigen B exhibit helper T-cell activity when assayed in an adoptive transfer system with spleen cells from mice primed with timothy pollen extract and treated with anti-thy 1.2 serum and complement. These helper cells, when placed in culture with antigen B, secrete a soluble antigen B-specific helper factor (THF) that requires the presence of normal spleen cells or cells that do not adhere to nylon wool in order to exhibit T cell-replacing activity. The THF has been partially purified over an immobilized antigen adsorbent. The eluted THF exhibits a mol. wt of 65,000 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and contains 5% carbohydrate by weight. The THF is I-Ak-positive and Ig-negative.

Animals↗