Antigen B-specific helper factor from timothy grass pollen: isolation and partial characterization.

C J Brandt; L B Deppe; A Malley

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PubMed · 6795110

Antigen B-specific helper factor from timothy grass pollen: isolation and partial characterization.

C J Brandt·L B Deppe·A Malley

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Abstract

Spleen cells from LAF1 mice primed with photo-oxidized antigen B exhibit helper T-cell activity when assayed in an adoptive transfer system with spleen cells from mice primed with timothy pollen extract and treated with anti-thy 1.2 serum and complement. These helper cells, when placed in culture with antigen B, secrete a soluble antigen B-specific helper factor (THF) that requires the presence of normal spleen cells or cells that do not adhere to nylon wool in order to exhibit T cell-replacing activity. The THF has been partially purified over an immobilized antigen adsorbent. The eluted THF exhibits a mol. wt of 65,000 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and contains 5% carbohydrate by weight. The THF is I-Ak-positive and Ig-negative.

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Animals Antibody Formation Cells, Cultured Chromatography, Affinity Immunoglobulin E Interleukin-1 Mice Pollen Proteins Spleen T-Lymphocytes Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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C J Brandt, L B Deppe, A Malley. 1981. Antigen B-specific helper factor from timothy grass pollen: isolation and partial characterization.. https://pubmed.ncbi.nlm.nih.gov/6795110/

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