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Biomedical subjects

L Cloarec

Publications and source records attributed to L Cloarec.

At least 37 records · Page 2Linked to original sources
PubMed1988

Level of plasma fibronectin in patients with breast cancer.

Plasma fibronectin was determined by laser nephelometric immunoassay in two populations: healthy individuals and patients with metastatic or non-metastatic breast cancer. The results showed that the fibronectin concentration was higher in the patient group than in the healthy controls of similar age, with a significant difference (p less than 0.05). The patients who had metastatic breast cancer tended to show higher levels than those with no detectable metastasis, but such a difference was not statistically significant. Since fibronectin is sensitive to clinical events unrelated to the malignancy status, it does not seem suitable as a tumor marker.

Adolescent↗
PubMed1987

[Use of monoclonal antibodies for the immunoenzymological determination of alpha-fetoprotein in amniotic fluid].

A two site immunoenzymatic assay using monoclonal antibodies for alpha-fetoprotein in amniotic fluid has been evaluated. Results obtained with monoclonal antibodies correlated well with those obtained by electroimmunodiffusion assay. The evaluation of this method has shown rapidity, accuracy and a better sensitivity than electroimmunodiffusion assay. Further more the measurements of both maternal serum and amniotic fluid alpha-fetoprotein can be associated.

Amniotic Fluid↗
PubMed1986

[Use of monoclonal antibodies for the immunoenzyme determination of creatine kinase-isoenzyme MB].

An enzyme immunoassay using monoclonal antibodies for creatine kinase-MB in human serum is described. The specificity and the linearity have been studied. The coefficient of within assay ranges from 6% to 22.5% according to the concentrations. The coefficient of between-assay ranges from 8% to 16.6%. Compared to electrophoresis, the results obtained with the enzyme immunoassay are a better sign of the physical state of the patients.

Antibodies, Monoclonal↗
PubMed1985

[Prenatal diagnosis of abnormalities of the neuroectodermal tube. Limitations of the assay of amniotic alpha fetoprotein and acetylcholinesterase and value of the qualitative study of cholinesterases. Apropos of 6 cases of anencephaly].

Measurement of amniotic fluid alpha-foetoprotein and acetylcholinesterase for the detection of neural tube defects has been used in 285 normal or neural tube defects affected pregnancies: 18 false positive results (with non neural tube defects fetal malformations) have been reported. A qualitative polyacrylamide gel electrophoresis technique has been used to identify the acetylcholinesterase isoenzyme derived from foetal cerebrospinal fluid. Results illustrate the value of amniotic fluid acetylcholinesterase electrophoresis. Amniotic cholinesterase presence may serve as a valuable marker for the detection of neural tube defects.

Acetylcholinesterase↗
PubMed1985

[Amniotic fibronectin in normal pregnancy and pregnancy with fetal anencephaly].

Fibronectin, which is an important protein in plasma and in surface cells was calculated in amniotic fluid using a laser ray nephelemetric technique. In normal pregnancies (in 72 samples studied) this protein which rises towards the 18th week of amenorrhoea (mean +/- S.D.: 60.8 +/- 16.0 mg/l), decreases steadily to term (mean 14.8 +/- 8.9 mg/l). In fetal anencephaly (10 samples tested) high levels were also found but the difference as compared with normal levels was not significant. This measurement does not therefore constitute an extra way of screening for closure of the neural tube.

Amniotic Fluid↗
PubMed1984

[Abnormalities of the central nervous system. Results of 3 years of genetic counseling at the University Hospital of Rennes: 192 cases].

The authors present their view of screening for central nervous system malformations in Brittany, having studied 192 case histories of subjects seen in the three years of genetic counselling in Rennes. Ultrasound usually manages to demonstrate anencephaly but all too often it fails to demonstrate spina bifida. Furthermore serum or amniotic fluid alphafetoprotein levels are often poorly interpreted. Microcephaly and encephaloceles occur rarely. The ultrasound diagnosis of the latter is easy whereas it is more difficult to diagnose microcephaly. The authors point out that there are familial forms of hydrocephaly and of holoprosencephaly which are not all that rare and fairly easy to diagnose so long as one remembers this very serious abnormality.

Academic Medical Centers↗
PubMed1984

[Critical review of 243 monoclonal immunoglobulinopathies].

243 cases of monoclonal immunoglobulinopathy were seen at the CHR in Rennes over a period of three years. The authors studied successively the relationship between the monoclonal immunoglobulin and the clinical diagnosis the distribution of the monoclonal compounds according to the heavy chain class and the identification of the light chains, the distribution of the immunoglobulinopathies in terms of the age and the sex of the patients and the correlation between the clinical diagnosis and various laboratory findings (proteins concentration, concentration of normal immunoglobulins, sedimentation rate...). The results obtained are in line with the principal data in the literature concerning monoclonal immunoglobulinopathies. However, the authors demonstrated, in the studied population, a higher percentage of monoclonal IgM than is usually reported. Furthermore, these IgM immunoglobulins, present in Waldenstrom's disease and frequently seen in various other immunoglobulinopathies, were essentially found in men.

France↗
PubMed1983

[Changes in blood levels of haptoglobin and ceruloplasmin in vitamin A deficiency in Sprague-Dawley rats].

The aim of the present study was to find out whether the elevation of the serum ceruloplasmin level, previously described in vitamin-A-deficient rats, is a specific phenomenon. Quantitative variations of serum ceruloplasmin, albumin and haptoglobin (whose concentration increased during inflammation) were determined in normal and vitamin-A-deficient rats. Concentrations of ceruloplasmin, haptoglobin, and the value of the haptoglobin to albumin ratio are increased in the serum of vitamin-A-deficient rats compared to normal rats. The results suggested that the increased serum level of ceruloplasmin in vitamin-A-deficient rats was due to the presence of inflammation.

Animals↗
PubMed1982

[Ceruloplasmin determination in the inflammatory reaction and during the last three months of pregnancy : choice of a technique (author's transl)].

Two methods of ceruloplasmin determination have been tested when this protein reaches its highest levels : inflammatory reaction and the last three months of pregnancy. A very good agreement between a spectrophotometric method based on oxydasis properties of ceruloplasmin and an immunoprecipitation was found in the first case. However during the last months of pregnancy, the data obtained by immunoprecipitation yield low values when compared to spectrophotometry. This results confirmed by experiments with rats indicate that the spectrophotometric method should be preferred in all circumstances.

Animals↗
PubMed1982

[The levels of apolipoprotein A in liquor in normal and pathological pregnancies (author's transl)].

As liquor contains only HDL (High Density Lipoprotein) as a lipoprotein we have studied the changes in the levels of apolipoprotein A, which is the major component of HDL according to the duration of the pregnancy. The study has been carried out in normal and pathological pregnancies. It has been found that the level of apolipoprotein A rises from the 16th week of amenorrhoea of pregnancy to the 26th week and then gradually drops to term. The maximum level is approximately ten times greater than the level of apolipoprotein A at term (a level approximately of 1 mg per litre). This change parallels that of total proteins throughout pregnancy. We have limited our study in pathological pregnancies to the examination of the liquor at the end of pregnancy. The three pathological maternal conditions that have been most frequently found are:--diabetes,--Rhesus-immunisation,--vasculo-renal syndromes. There has been no significant change shown up in the period that we have studied, which was from the 30th to the 38th week of amenorrhoea.

Amniotic Fluid↗
PubMed1980

[Amniotic alpha 1-foetoprotein of the 17th week of pregnancies with amenorrhea (author's transl)].

The amniotic alpha 1-foetoprotein of the 17th week of pregnancies with amenorrhea was tested by electroimmunodiffusion and radioimmunology. The results show a very good correlation between both methods. We can see from this study the possibility for any laboratory to test alpha 1-foetoprotein in amniotic fluid of patients with high risk pregnancies, as the beginning of pregnancy when amniotic punction is necessary without using radioimmunology.

Amniotic Fluid↗
PubMed1980

[Isolation and physico-chemical properties of rat ceruloplasmin].

Ceruloplasmin was isolated from inflammatory rat serum after two chromatographies, employing column chromatography with D.E.A.E. cellulose and Sephadex G 200, and after electrophoresis. The determination of phys'co chemical constants allows to compare the human ceruloplasmin.

Animals↗
PubMed1979

[Relation between ceruloplasmin and vitamin A in Sprague-Dawley rats].

The Moore's hypothesis concerning a relationship between the metabolism of copper and that of vitamin A led us to consider a possible relationship between this vitamin and ceruloplasmin, the carrier protein for copper. Experiments were carried out on Sprague-Dawley rats. The ceruloplasmin level of control animals and vitamin A - deficient rats was determined An average increase between 22 and 33% was observed in the animals with vitamin A deficiency, the highest levels being observed in the females. These results are in agreement with Peterson's previous work. A second type of experiment was carried out to confirm these results. The rats were raised in a copper-deficient diet and the vitamin A level was regularly determined. In these conditions serum and liver levels of vitamin A are normal and do not vary, ceruloplasmin level is zero; however apoceruloplasmin is still being synthetized : Nevertheless at the ultime state of the deficiency vitamin A level decreases while apoceruloplasmin increases. These results raise the following question. Could the relationship between ceruloplasmin and vitamin A be possibly due to an inflammatory state in vitamin A deficient rats. In this respect a third series of experiments were undertaken and are in progress; the vitamin A and ceruloplasmin levels are regularly determined.

Animals↗
PubMed1977

[Inherited and familial bisalbuminemia. Acquired transient bisalbuminemia (author's transl)].

The present study is a survey of the different types of bisalbuminemia. In the inherited and familial form, the anomaly is fortuitously discovered and not associated with disease. The abnormal albumin fraction only differs from the normal one in a slight alteration in the aminoacid sequence, responsible for increased ("fast type") or decreased ("slow type") electrophoretic mobility. The anomaly is genetically determined and is transmitted as an autosomal codominant character. The condition is relatively rare, but has been observed in most parts of the world, with some higher incidence in many American Indian tribes. In the acquired transient biaslbuminemia, the abnormal component of the albumin is always of the fast type and occurs in patients either receiving large amounts of beta-lactamine type antibiotics, or suffering from pancreas diseases.

Anti-Bacterial Agents↗